Multiple-DOF (degree of freedom) adjusting mechanism of optical surface plasma resonance biosensor

Abstract

The invention relates to a multiple-DOF (degree of freedom) adjusting mechanism of an optical SPR (surface plasma resonance) biosensor. The mechanism comprises a laser regulating unit, a biochip component, a CCD (charge coupled device) regulating unit, a support plate and a base used for mounting the support plate, wherein the laser regulating unit and the CCD regulating unit are symmetrically arranged at the two sides of the prism bracket, and certain light paths can be formed among a laser, the biochip component and a CCD by adjusting the relative position of the laser regulating unit and the CCD regulating unit. The mechanism provided by the invention has the advantages that the structural design is delicate, and the location is accurate, thus the consistency of the light paths among the laser, the biochip component and the CCD can be conveniently adjusted; the pressure between a flow cell and the biochip component can be conveniently adjusted; the flow cell can maintain to be circulated after being lifted and reset and the accurate location of the biochip component can be guaranteed; the problems that debugging is difficult and the refractive index is difficult to match as the biochip provided with a glass substrate is replaced only are solved, the analysis accuracy of the biosensor is improved, and the use cost and the maintenance cost of the optical SPR sensor are greatly reduced.

Description

technical field [0001] The invention relates to the field of optical surface plasmon resonance (Surface plasmon resonance, SPR) biosensors, in particular to an optical SPR biosensor multi-degree-of-freedom adjustment mechanism. Background technique [0002] In the past 10 years, optical SPR biosensing technology has achieved rapid development in terms of implementation methods, instrument development and application field expansion. The detection mechanism of the optical SPR biosensor is as follows: the recognition film of biomolecules is fixed on the surface of the Au film of the optical SPR sensor, and the molecular recognition film captures the measured analyte, causing the refractive index of the surface of the sensor Au film to change, so it can be detected without labeling molecules. Test directly to realize non-destructive real-time online detection. Optical SPR biosensors include optical prism type, optical waveguide type, grating type, etc. according to the body of...

AI Technical Summary

Problems solved by technology

For example, the Chinese patent document CN101539570A discloses a coupling device for an optical SPR biochip, a sensor, and a microfluidic cell, but the device can only be coupled with an integrated biosensor imported from abroad, resulting in a high overall cost of the instrument, which will inevitably increase the use of users , maintenance cost, difficult to be widely used

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