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Biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection

A cytomegalovirus and biochip technology is applied in the field of biochips for rapid detection of pre-pregnancy intrauterine infection of Toxoplasma gondii and cytomegalovirus, which can solve the problem of high false positive rate

Active Publication Date: 2012-01-11
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, according to statistical data, ELISA and gold standard methods are commonly used in clinical diagnosis in my country to detect serum levels of TOX-IgG and CMV-IgG in pregnant women, and the false positive rate is high, which cannot provide accurate diagnostic basis for clinical use.

Method used

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  • Biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection
  • Biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection
  • Biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection

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Embodiment

[0157] The SPR biosensor is provided by Beijing Jinbojia Medical Technology Co., Ltd. The specific instrument model is UMPHO A600 / B, and the biochip is provided by Beijing Jinbojia Medical Technology Co., Ltd. The specific chip model is similar to the UMPHO A600 / B SPR biosensor match, such as figure 1 As shown, it includes a glass substrate 1, a gold film 2, a connection layer 3, and a surface substrate 4. A gold film 2, a connection layer 3 and a surface substrate 4 are attached to the glass substrate 1 in sequence, and there are 4 or 8 on the gold film 2. A diversion pool, the flow pool is coated with the aptamer mixed with SEQ ID No.1-SEQ ID No.3 and SEQ ID No.4-SEQ ID No.6. In the process of making the chip, the gold film 2 was pretreated first, that is, soaked and cleaned in 1.0mol / L NaOH for 20 minutes, washed three times with deionized water after taking it out, then put it in Piranha solution for 15 minutes, and immediately put it into a Soak in water and ethanol for...

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Abstract

The invention discloses a biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection. Compared with an antibody in traditional detection technique, an oligonucleotides adapter has the advantages that the binding force between the oligonucleotides adapter and target molecule is higher, stability is good, accuracy is high, repeatability is good, screening period is shorter, can be repeatedly used, can be stored for long time, can be precisely modified at sites, and the like; and by placing the obtained biological chip in a SPR (surface plasmon resonance) biosensor for detection, (1) the real-time detection is realized, (2) the sample does not need to be labeled, (3) the consumption of the sample is extremely low, (4) the detection processis convenient and efficient, and has high sensitivity, (5) the application range is very broad, (6) the flux is high, (7) in most cases, the sample does not need to be pretreated, and (8) the turbid or even nontransparent samples can be detected.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a biochip for rapid detection of toxoplasma gondii and cytomegalovirus in the uterus before pregnancy. Background technique [0002] Birth defects bring great mental and economic burdens to families, countries and society. At present, there is no effective treatment for fetal congenital defects. Among the methods of preventing birth defects, the primary prevention in the first trimester is the most effective. Toxoplasma gondii and cytomegalovirus are a group of pathogenic microorganisms infected during pregnancy, which can cause intrauterine infection through the placenta or birth canal, leading to miscarriage, stillbirth or fetal growth retardation, congenital malformations, neonatal infection and growth and development disorders of infants and young children. About 26,000 children with mixed infection of Toxoplasma gondii and cytomegalovirus are born in my country every...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12Q1/04
Inventor 刘星府伟灵
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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