50 results about "First trimester" patented technology

The present invention provides a novel approach for identification and characterization of differentially represented fetal or maternal genomic regions in maternal circulation. Identification of overrepresented fetal genomic regions in the maternal circulation according to the present invention permit accurate analysis of fetal DNA without the need for enrichment or purification, which provides a simpler, more accurate and efficient prenatal diagnosis in early pregnancy. The present invention is particularly useful for noninvasive prenatal diagnosis during early pregnancy (e.g., during the first trimester).

A method of isolating a pluripotent cell from human umbilical cord is described herein. The method involves collecting a sample of umbilical cord from fetal tissue obtained at less than 20 weeks of gestation, for example a first trimester umbilical cord. The sample is treated to obtain isolated umbilical cord cells, after which the isolated umbilical cord cells are incubated. Stem cells obtained in this way can be differentiated for use in therapeutic applications.

The invention relates to a sow feeding method of a special wild pig. The method comprises the following steps: (1) feeding sows to reach sexual maturity when the sows are 6-7 months old and are 75-80 kg weight and reach body maturity when the sows are 7-8 months old and 80-90 kg weight and mating the sows; feeding and mating 4-6 non-pregnant sows in each pigsty; feeding each sow at the first trimester of pregnancy in a limiting pigsty or in an independent pigsty, 2-4 sows at the mid trimester of pregnancy in each pigsty and 1-2 sows at the later trimester of pregnancy in each pigsty, and feeding the sows on obstetric tables from 7 days before parturition to weaning; (2) reinforcing sow feeding management, meeting nutritional requirements in the non-pregnant to-be-mating period, the first trimester of pregnancy, the mid trimester of pregnancy, the later trimester of pregnancy and the suckling period, preparing daily rations for the different periods, feeding different feeds, and paying attention to rational matching of green and coarse fodders and concentrated feeds. According to the sow feeding method, the feed and the control for feeding are optimized, so that the sows rut quickly, the farrowing rate is high, normally 12-14 piglets are born by each sow, and the survival rate is over 90 percent.

The invention provides improved assays for detection of pregnancy. In the assays, pregnancy associated glycoproteins are analyzed in conjunction with progesterone analysis. The techniques of the invention overcome limitations in the prior art by reducing the rate of false positive results. The assays provided by the invention can be implemented to increase the efficiency of commercial animal breeding programs.

Gene expression patterns contemporaneous with early placental development in the first trimester of preeclamptic versus unaffected pregnancies have been obtained. Observation of differences in these gene expression patterns has allowed the identification of biomarkers that are useful in predicting and monitoring preeclampsia. These biomarkers are also useful in screening potential therapeutics for efficacy in the prevention or treatment of preeclampsia.

The invention discloses application of a compound traditional Chinese medicine composition to preparation of an inducing solution for directionally differentiating first trimester-derived perivascular cells as egg-like cells. The compound traditional Chinese medicine composition comprises the following bulk drugs in parts by weight: 18 to 30 parts of prepared rehmannia rhizome, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of barbary wolfberry fruit, 8 to 16 parts of south dodder seed and 8 to 16 parts of deer-horn glue. The invention also discloses the inducing solution and an inducing method for directionally differentiating the first trimester-derived perivascular cells as the egg-like cells. By virtue of the inducing solution, the first trimester-derived perivascular cells can be induced to be directionally differentiated as the egg-like cells. The inducing solution has great clinical application prospect.

The subject invention pertains to biomarkers for identifying a subject as having high risk of the development PE. The biomarkers presented herein include miRNAs, post-translational modification of histone proteins, amount, expression and / or activity of histone or DNA modifying enzymes and methylation of sites in the genomic DNA. In certain embodiments, increased miR-17, increased acetylation of H4 histone protein, decreased amount, expression and / or activity of HDAC5 mRNA or protein or increased methylation of DNA at the genomic site CYP19A1 in the blood, serum or plasma of a subject compared to that of a control subject is used to predict the development of PE in the subject. The invention also provides kits and reagents to conduct assays to quantify biomarkers described herein. The invention further provides the methods of treating and / or managing PE in a subject identified as having a high risk of the development of PE.

The invention relates to a enzyme predicted to be a serine protease, which is specifically expressed in association with embryo implantation and placentation in pregnant uterus. The enzyme of the invention is useful in the evaluation of fertility and monitoring of early pregnancy, placental development and function, fetal development, parturition, and conditions such as pre-eclampsia, intrauterine growth restriction, early abortion, abnormal uterine bleeding, endometriosis, and cancers, and may provide a potential target for contraception. It may also be important in diseases of the heart, testis or ovary, and may play a role in muscle function, including cardiac muscle, skeletal muscle, lung and the diaphragm. In addition the enzyme of the invention is useful in the screening of candidate drugs for fertility control or for treatment of fertility-related disorders.

The invention provides serum-based, diagnostic, biological assays for predicting disorders of pregnancy resulting from poor trophoblast and / or placental ischemia, including preeclampsia. Serum samples from such subjects exhibit an ability to disrupt the architecture involving fetal trophoblasts and maternal endothelial cells in a three-dimensional, dual cell co-culture system provided herein, in contrast to normal pregnancy serum samples. Based on these distinctions, the assays are employed to predict pregnancy outcomes as early as first trimester.

The invention discloses an exosome miRNA marker for early pregnancy diagnosis of ewes and application of the exosome miRNA marker. The exosome miRNA molecular marker comprises oar-miR-22-3p and chi-miR-378-3p. The miRNA molecular marker shows high expression in serum exosomes of ewes in early pregnancy, and when the serum exosome miRNA is independently used for diagnosing and distinguishing the ewes in early pregnancy from the ewes in non-pregnancy, ROC area under the curve (AUC) is 0.891 (0.728, 1.000), 0.922 (0.791, 1.000), respectively; the AUC (95% CI) of the exosome miRNA molecular markeris 0.984 (0.936, 1.000) when early pregnant ewes and non-pregnant ewes are distinguished through combined diagnosis, it can be seen that the exosome miRNA molecular marker has high sensitivity and high specificity in early ewe pregnancy diagnosis, multiple miRNA markers are combined, and superior diagnosis performance is obtained.

The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.

Provided is a non-invasive prenatal testing method based on whole-genome tendency scoring and adapted to test whether a pregnant woman's fetus has autosomal aneuploidy. The method includes: creating a database of mk values which equal the averages of length proportions of health persons' chromosome k; obtaining the pregnant woman's and her fetus' chromosomal data yk from the pregnant woman's plasma; obtaining p values by the ratio of the yk values to the mk values; and analyzing p values to determine whether the target chromosome has chromosome aneuploidy. The comparable data is increased and thus test accuracy is enhanced by comparing the p values and the p values in a database. Due to an abundance of comparable data, the method of the present invention is accurate even when performed in the first trimester, such that pregnant women can know as soon as possible whether their fetuses' chromosomes are normal.

A method of isolating a pluripotent cell from human umbilical cord is described herein. The method involves collecting a sample of umbilical cord from fetal tissue obtained at less than 13 weeks of gestation, for example a first trimester umbilical cord. The sample is treated to obtain isolated umbilical cord cells, after which the isolated umbilical cord cells are incubated. Cells obtained in this way can be differentiated for use in treating conditions of cell damage, by supplanting the function of a damaged cell in a condition such as spinal cord injury, cardiovascular injury or heart disease.

The invention relates to a method for detecting early pregnancy of sheep. The method comprises the following steps of: collecting sheep blood or urine from the seventh day to the fourteenth day aftermating of ewes; inserting sheep chorionic gonadotropin HCG detection test paper into serum or urine for 5-10 seconds, and after 15-18 minutes, indicating that a detection result is invalid if a test paper quality control line C does not develop color and a detection line T develops color; indicating that an HCG level of ewes is low if the quality control line C develops color and the detection line T does not develop color; comparing developed colors with an HCG colorimetric card if the quality control line C and the detection line T both develop colors; indicating that the ewes are not pregnant if HCG is smaller than 10 mIU / ml; indicating that the ewes are possibly pregnant and need to be continuously detected twice if the HCG is greater than or equal to 10 mIU / ml, and indicating that theewes are pregnant and have living embryos if the HCG of twice detection rises and the HCG detected later is 1-2 times of the HCG detected last time; and indicating that ewes are pregnant but encounter embryo damage if the HCG of twice detection is kept unchanged or decreased, and taking miscarriage prevention measures or stopping pregnancy.

The invention provides a method for detecting early pregnancy of cows. The method comprises the following steps of: collecting morning urine of cows or urine urinated 1-2 hours after last urination onthe seventh day to the fourteenth day after cows are mated; inserting HCG detection test paper of the cows into urine for 5-10 seconds, and indicating that a detection result is invalid if a test paper quality control line C does not develop color and a detection line T develops color; indicating that the HCG level of cows is low if the quality control line C develops color and the detection lineT does not develop color; comparing developed colors with an HCG colorimetric card if the quality control line C and the detection line T both develop colors; indicating that the cows are not pregnant if the HCG is less than 10mIU / ml; indicating that the cows are possibly pregnant and need to be continuously tested twice if HCG is greater than or equal to 10 mIU / ml; indicating that the cows are pregnant and have living embryos if the HCG of twice detection rises and the HCG detected later is 1-2 times of the HCG detected last time; and indicating that cows are pregnant but encounter embryo damage if the HCG of twice detection is kept unchanged or decreased, and taking miscarriage prevention measures or stopping pregnancy in time.

The invention provides serum-based, diagnostic, biological assays for predicting disorders of pregnancy resulting from poor trophoblast and / or placental ischemia, including preeclampsia. Serum samples from such subjects exhibit an ability to disrupt the architecture involving fetal trophoblasts and maternal endothelial cells in a three-dimensional, dual cell co-culture system provided herein, in contrast to normal pregnancy serum samples. Based on these distinctions, the assays are employed to predict pregnancy outcomes as early as first trimester.

The invention provides an early pregnancy risk prediction method and system based on multi-source data and a medium. Firstly, basic information, physiological cycle data and living habit data of a user are collected through a preset interface; preprocessing the original data to generate standardized structure data; extracting age features, periodic regularity features and living habit features to obtain a feature vector set; then, sequentially comparing the age feature with a first threshold value to generate a risk index, comparing the physiological cycle feature with a second threshold value to update the risk index, and matching the living habit feature with a third threshold value to obtain feature matching information; integrating the risk index and the matching information based on a weighted fusion algorithm to generate a pregnancy risk score; and finally, generating a visual risk interface through a graphic rendering engine in combination with historical data, and outputting the visual risk interface. Therefore, comprehensive assessment and real-time monitoring of pregnancy risks are realized, and the accuracy of health management and the effectiveness of decision support are improved.

The invention provides a method for establishing a horseshoe kidney animal model. The method comprises: injecting a certain amount of retinoic acid solution at one time in the early pregnancy of the pregnant animal, and routinely feeding and giving birth to obtain the horseshoe kidney animal model. The method for creating an animal model of horseshoe kidney of the present invention has good experimental repeatability, and provides an experimental tool and platform for mechanism research for abnormal kidney development such as horseshoe kidney. Surgical operation and drug evaluation provide an extremely close and convenient experimental tool platform and scientific basis.

The invention discloses a primer for diagnosis of early pregnancy of sows. The nucleotide sequence of the primer is shown as follows: SRY-O-F: 5 '-CAGCAAAATATTCTCGCCTTGG-3', and the nucleotide sequence of the primer is shown as follows. And SRY-O-R: 5 '-CATCCTCTCTCTACGC-3', and SRY-O-R: 5 '- SRY-I-F: 5 '-ATATTCTCGCCTTGGGG-3', SRY-I-F: 5 '- And SRY-I-R is 5 '-GCTTTCGGCTTCTGTA-3', and the formula is shown in the description. Two pairs of primers with high sensitivity and strong specificity are designed through comparative analysis of pig SRY gene sequences, fetal free DNA in peripheral blood of sows is used as a template, and the specificity and sensitivity of PCR are enhanced through amplification of the two pairs of primers. Through two rounds of PCR amplification, whether the sow is pregnant or not can be detected on the 18th day of pregnancy, and the accuracy of the detection result is extremely high and reaches 100%. Compared with the prior art that the ZFY gene is used for detecting the sows on the twentieth breeding day, the method has the advantages that the detection time can be advanced to the eighteenth breeding day, and the detection accuracy is greatly improved (100% vs 10%). According to the method, powerful technical support is provided for early and accurate identification of the non-pregnant sows, reduction of non-production days and feeding cost and smooth execution of a production plan.

The invention discloses a biomarker for predicting pregnancy outcome of an infertility patient and application of the biomarker, and belongs to the technical field of biology and reproductive medicine. The biomarker for predicting the pregnancy outcome of the infertility patient is a quantity ratio of T-bet molecules to GATA3 molecules. Experiments show that the quantity ratio of the T-bet molecules to the GATA3 molecules can be used as a biomarker for predicting pregnancy outcome of infertility patients, and the biomarker can be used as a prediction and prevention target for early pregnancy failure of the infertility patients, and has positive pharmaceutical value and wide social significance. In addition, the invention also discloses a kit of the biomarker for predicting the pregnancy outcome of the infertility patient.

A pregnancy monitoring device and method improve detection in the first trimester of the occurrence of ectopic pregnancy and the occurrence of multiple foetuses. The device includes a sample absorbing member and an analyser to analyse a plurality of sequential a biological liquid samples and computing means to determine an ovulation date and deviations in the measured quantity of hCG from predetermined values which are indicators of ectopic pregnancy or multiple pregnancy. The device and method enables early detection of ectopic pregnancy or multiple pregnancy.

A method of isolating a pluripotent cell from human umbilical cord is described herein. The method involves collecting a sample of umbilical cord from fetal tissue obtained at less than 13 weeks of gestation, for example a first trimester umbilical cord. The sample is treated to obtain isolated umbilical cord cells, after which the isolated umbilical cord cells are incubated. Cells obtained in this way can be differentiated for use in treating conditions of cell damage, by supplanting the function of a damaged cell in a condition such as spinal cord injury, cardiovascular injury or heart disease.

The invention discloses a novel biomarker for early abortion diagnosis, and particularly relates to a method for diagnosing, predicting and / or monitoring early abortion by hsa-miR-29a-3p and application of hsa-miR-29a-3p. According to the invention, hsa-miR-29a-3p is proposed as a marker for early pregnancy embryo implantation and bad pregnancy outcome for the first time, and then the regulation effect of hsa-miR-29a-3p on decidualization is proved by using an in-vitro endometrial stromal cell decidualization model. The temporal-spatial expression specificity based on the marker has wide clinical diagnosis and prediction application prospects. According to the specific expression of the hsa-miR-29a-3p in different pregnancy periods and in decidua tissues of early abortion, the early abortion of pregnancy can be effectively predicted and diagnosed.

The invention provides an application of metabolites in sow vaginal secretion in early pregnancy diagnosis, and finds that three metabolic molecules can be used as markers for the early pregnancy diagnosis of sows for the first time on the basis of non-targeted metabonomics and machine learning conjoint analysis. According to the present invention, the metabolic markers are respectively INDOLEPROPIONIC ACID (indolepropionic acid), cis-Amonitate (cis-Amonitate) and 4-p-Coumaroylquinic acid (4-p-coumaroylquinic acid), the ROC curve AUC values of the metabolic markers are respectively 0.88, 0.92 and 0.77, and the metabolic markers have excellent diagnosis performance during the combination diagnosis. The pregnancy diagnosis is carried out by adopting the vaginal secretions of the sows 18 days after hybridization, the effective pregnancy diagnosis time is advanced by 5-8 days on the basis of a wave diagnosis method, and the method has important significance for reducing cost and increasing ultrasonic efficiency in live pig feeding.

The invention discloses a research method for traditional Chinese medicine physique distribution characteristics of nausea and vomiting in a gestation period, and belongs to the technical field of medical research. The method comprises: selecting a certain number of pregnant women at an early pregnancy stage as research objects; carrying out physique analysis on the research objects to obtain thephysique data of the research objects, wherein the physique analysis content comprises gentle physique analysis, yang deficiency physique analysis, yin deficiency physique analysis, qi depression physique analysis, qi deficiency physique analysis, damp-heat physique analysis, phlegm-damp physique analysis, allergic physique analysis and blood stasis physique analysis; and correspondingly performing data analysis on the physique data and the nausea and vomiting conditions of the research objects in a gestation period to obtain the traditional Chinese medicine physique distribution characteristic rule of nausea and vomiting in the pregnancy period is obtained. With the method, through physique detection, before pregnancy, the possible nausea and vomiting possibly occurring in an early pregnancy stage of women at an early pregnancy stage can be predicted and judged, and the corresponding suggestions are provided, so that adverse effects caused by nausea and vomiting in early pregnancy stage are reduced.

The invention discloses a biomarker for predicting or diagnosing early pregnancy of sows and application of the biomarker. The biomarker is selected from at least one of miRNA (micro Ribonucleic Acid) novel-235, miRNA novel-878 and miRNA novel-1215. The invention further discloses a preparation method of the biomarker. These biomarkers exhibit an expression difference in saliva extracellular vesicles of a sow population, and this expression difference is associated with sow pregnancy. The miRNAs are developed into molecular markers for early detection of sow pregnancy, and the pregnancy condition can be conveniently detected. The embodiment of the invention further provides a primer and a kit for rapid detection of early pregnancy of the sow, and a new way is provided for diagnosis of early pregnancy of the sow.

1. Name of the product in this design: Pregnancy test stick. 2. Purpose of this design: For early pregnancy diagnosis. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The present invention relates to a combination of valproic acid (VPA) and S- adenosylmethionine (SAMe) for use in preventing congenital malformations in a fetus of a subject in need of VPA treatment and who is pregnant or intends to become pregnant, by administering the combination to the subject before or during the first trimester of pregnancy, wherein the SAMe and the VPA are at a ratio of about 1:10-1:50 w / w SAMe:VPA in the combination; and corresponding methods of treatment.