Method for detecting early pregnancy of cows

Abstract

The invention provides a method for detecting early pregnancy of cows. The method comprises the following steps of: collecting morning urine of cows or urine urinated 1-2 hours after last urination onthe seventh day to the fourteenth day after cows are mated; inserting HCG detection test paper of the cows into urine for 5-10 seconds, and indicating that a detection result is invalid if a test paper quality control line C does not develop color and a detection line T develops color; indicating that the HCG level of cows is low if the quality control line C develops color and the detection lineT does not develop color; comparing developed colors with an HCG colorimetric card if the quality control line C and the detection line T both develop colors; indicating that the cows are not pregnant if the HCG is less than 10mIU/ml; indicating that the cows are possibly pregnant and need to be continuously tested twice if HCG is greater than or equal to 10 mIU/ml; indicating that the cows are pregnant and have living embryos if the HCG of twice detection rises and the HCG detected later is 1-2 times of the HCG detected last time; and indicating that cows are pregnant but encounter embryo damage if the HCG of twice detection is kept unchanged or decreased, and taking miscarriage prevention measures or stopping pregnancy in time.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting early pregnancy in cattle, and belongs to the technical field of animal detection methods. Background technique [0002] At present, the pregnancy detection of cattle mainly includes external observation method and test method, as well as ultrasonic detection method, immunological detection method, red blood cell agglutination test, serum acid titration method and so on. The external observation method and the estrus test method usually detect whether the cow is pregnant by observing whether the cow returns to estrus 17-21 days after mating, but the cow embryo stops developing, and the cow will not return to estrus in a short period of time, and will in a pseudopregnancy state. Ultrasonic diagnostic method is a kind of physical detection that uses the physical characteristics of ultrasound to closely combine the physical characteristics of ultrasound with the acoustic...

AI Technical Summary

Problems solved by technology

However, due to the influence of these hormones in the serum of pregnant and non-pregnant cows due to factors such as breeds and individual differences, it is still difficult to promote them in production.

The erythrocyte agglutination test is to combine the specific antigen in the early pregnancy cow with red blood cells, and use it to prepare antiserum. When mixed with the 10-15-day pregnant cow erythrocytes, the erythrocyte agglutination will occur. If it is not pregnant, it will not occur. Erythrocyte agglutination, the accuracy rate of this method is 90% for cows at 28-60 days of pregnancy, but erythrocyte agglutination will also occur after the cow embryo stops developing, which will easily lead to the long-term pseudo-pregnancy state of the cow

Serum acid titration means that during the rapid growth of embryos in cows, the body produces a unique immunoglobulin, which forms low-soluble complexes (floccules) with other proteins in an acidic environment, and the accuracy rate can reach 90.81% %, but after the ewe embryo stops developing, it will also form low-soluble complexes (flocs) with other proteins, and it is also easy to cause the cow to be in a state of false pregnancy for a long time. It can only be carried out at room temperature, which is not conducive to on-site detection

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