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Method for detecting early pregnancy of cows

A technology of early pregnancy and detection line, applied in the field of detection, can solve problems such as unfavorable on-site detection, false pregnancy, cumbersome operation process, etc., achieve the effect of shortening the number of non-breeding days, promoting intensive management, and improving breeding efficiency

Active Publication Date: 2020-08-28
昆明天沃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the influence of these hormones in the serum of pregnant and non-pregnant cows due to factors such as breeds and individual differences, it is still difficult to promote them in production.
The erythrocyte agglutination test is to combine the specific antigen in the early pregnancy cow with red blood cells, and use it to prepare antiserum. When mixed with the 10-15-day pregnant cow erythrocytes, the erythrocyte agglutination will occur. If it is not pregnant, it will not occur. Erythrocyte agglutination, the accuracy rate of this method is 90% for cows at 28-60 days of pregnancy, but erythrocyte agglutination will also occur after the cow embryo stops developing, which will easily lead to the long-term pseudo-pregnancy state of the cow
Serum acid titration means that during the rapid growth of embryos in cows, the body produces a unique immunoglobulin, which forms low-soluble complexes (floccules) with other proteins in an acidic environment, and the accuracy rate can reach 90.81% %, but after the ewe embryo stops developing, it will also form low-soluble complexes (flocs) with other proteins, and it is also easy to cause the cow to be in a state of false pregnancy for a long time. It can only be carried out at room temperature, which is not conducive to on-site detection

Method used

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  • Method for detecting early pregnancy of cows
  • Method for detecting early pregnancy of cows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The bovine chorionic gonadotropin HCG test paper is prepared by the following method:

[0042] 1) Prepare the components as follows:

[0043] 11) Gold solution: Add 2ml of chloroauric acid solution with a mass concentration of 1%, and sodium citrate with a mass concentration of 1% to 100ml of purified water, heat until the solution turns purple, and cool to room temperature to obtain a gold solution;

[0044] 12) Blocking solution: Dissolve 0.005g bovine serum albumin in 300ul purified water, add 30ul potassium carbonate solution, and mix well to obtain 330ul blocking solution;

[0045] 13) Sample pad treatment solution: completely dissolve 0.605g of tricarboxymethylaminomethane and 1.5g of sodium chloride in 100ml of purified water to obtain a sample pad treatment solution;

[0046] 14) Colloidal gold conjugate diluent: mix 0.605g tricarboxymethylaminomethane, 0.2g polyvinylpyrrolidone, 5g sucrose, 0.9g sodium chloride, 0.5g bovine serum albumin, 3ml sheep serum, 0.15...

Embodiment 2

[0064] The method for detecting early pregnancy of cattle comprises the following steps:

[0065] 1) On the 8th day after the cow is mated, collect 5ml of the cow's morning urine;

[0066] 2) Insert a chorionic gonadotropin HCG detection test paper of Example 1 into the urine of step 1) for 6 seconds, take it out, and observe whether the quality control line C and the detection line T in the test paper are significantly different after 16 minutes. color:

[0067] 3) The color development results are as follows:

[0068] The quality control line C of the test paper has no color, and the test line T has color, indicating that the test result is invalid;

[0069] Repeat steps 1) and 2) the next day, and observe whether the quality control line C and the detection line T in the test paper develop color. The color development results are as follows:

[0070] The quality control line C of the test paper develops color, but the test line T does not develop color, which indicates t...

Embodiment 3

[0072] The method for detecting early pregnancy of cattle comprises the following steps:

[0073] 1) On the 7th day after the ewes are mated, collect 10ml of urine that the ewe urinates 2 hours after the last urination;

[0074] 2) Insert a chorionic gonadotropin HCG detection test paper of Example 1 into the urine of step 1) for 9 seconds, take it out, and observe whether the quality control line C and the detection line T in the test paper are significantly different after 17 minutes. color:

[0075] 3) The color development results are as follows:

[0076] The quality control line C on the test paper develops color, but the test line T does not develop color, indicating that the level of chorionic gonadotropin HCG in the cow is low. On the third day, repeat steps 1) and 2) for detection, and the result shows: the test paper quality control line C and the detection line T are all colored, use this color and HCG color card as figure 2 As shown, the following comparisons a...

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Abstract

The invention provides a method for detecting early pregnancy of cows. The method comprises the following steps of: collecting morning urine of cows or urine urinated 1-2 hours after last urination onthe seventh day to the fourteenth day after cows are mated; inserting HCG detection test paper of the cows into urine for 5-10 seconds, and indicating that a detection result is invalid if a test paper quality control line C does not develop color and a detection line T develops color; indicating that the HCG level of cows is low if the quality control line C develops color and the detection lineT does not develop color; comparing developed colors with an HCG colorimetric card if the quality control line C and the detection line T both develop colors; indicating that the cows are not pregnant if the HCG is less than 10mIU / ml; indicating that the cows are possibly pregnant and need to be continuously tested twice if HCG is greater than or equal to 10 mIU / ml; indicating that the cows are pregnant and have living embryos if the HCG of twice detection rises and the HCG detected later is 1-2 times of the HCG detected last time; and indicating that cows are pregnant but encounter embryo damage if the HCG of twice detection is kept unchanged or decreased, and taking miscarriage prevention measures or stopping pregnancy in time.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting early pregnancy in cattle, and belongs to the technical field of animal detection methods. Background technique [0002] At present, the pregnancy detection of cattle mainly includes external observation method and test method, as well as ultrasonic detection method, immunological detection method, red blood cell agglutination test, serum acid titration method and so on. The external observation method and the estrus test method usually detect whether the cow is pregnant by observing whether the cow returns to estrus 17-21 days after mating, but the cow embryo stops developing, and the cow will not return to estrus in a short period of time, and will in a pseudopregnancy state. Ultrasonic diagnostic method is a kind of physical detection that uses the physical characteristics of ultrasound to closely combine the physical characteristics of ultrasound with the acoustic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/558G01N33/532G01N33/543
CPCG01N33/76G01N33/558G01N33/532G01N33/54313G01N2333/59
Inventor 金义达
Owner 昆明天沃生物科技有限公司
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