Method for detecting early pregnancy of cows
A technology of early pregnancy and detection line, applied in the field of detection, can solve problems such as unfavorable on-site detection, false pregnancy, cumbersome operation process, etc., achieve the effect of shortening the number of non-breeding days, promoting intensive management, and improving breeding efficiency
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Embodiment 1
[0041] The bovine chorionic gonadotropin HCG test paper is prepared by the following method:
[0042] 1) Prepare the components as follows:
[0043] 11) Gold solution: Add 2ml of chloroauric acid solution with a mass concentration of 1%, and sodium citrate with a mass concentration of 1% to 100ml of purified water, heat until the solution turns purple, and cool to room temperature to obtain a gold solution;
[0044] 12) Blocking solution: Dissolve 0.005g bovine serum albumin in 300ul purified water, add 30ul potassium carbonate solution, and mix well to obtain 330ul blocking solution;
[0045] 13) Sample pad treatment solution: completely dissolve 0.605g of tricarboxymethylaminomethane and 1.5g of sodium chloride in 100ml of purified water to obtain a sample pad treatment solution;
[0046] 14) Colloidal gold conjugate diluent: mix 0.605g tricarboxymethylaminomethane, 0.2g polyvinylpyrrolidone, 5g sucrose, 0.9g sodium chloride, 0.5g bovine serum albumin, 3ml sheep serum, 0.15...
Embodiment 2
[0064] The method for detecting early pregnancy of cattle comprises the following steps:
[0065] 1) On the 8th day after the cow is mated, collect 5ml of the cow's morning urine;
[0066] 2) Insert a chorionic gonadotropin HCG detection test paper of Example 1 into the urine of step 1) for 6 seconds, take it out, and observe whether the quality control line C and the detection line T in the test paper are significantly different after 16 minutes. color:
[0067] 3) The color development results are as follows:
[0068] The quality control line C of the test paper has no color, and the test line T has color, indicating that the test result is invalid;
[0069] Repeat steps 1) and 2) the next day, and observe whether the quality control line C and the detection line T in the test paper develop color. The color development results are as follows:
[0070] The quality control line C of the test paper develops color, but the test line T does not develop color, which indicates t...
Embodiment 3
[0072] The method for detecting early pregnancy of cattle comprises the following steps:
[0073] 1) On the 7th day after the ewes are mated, collect 10ml of urine that the ewe urinates 2 hours after the last urination;
[0074] 2) Insert a chorionic gonadotropin HCG detection test paper of Example 1 into the urine of step 1) for 9 seconds, take it out, and observe whether the quality control line C and the detection line T in the test paper are significantly different after 17 minutes. color:
[0075] 3) The color development results are as follows:
[0076] The quality control line C on the test paper develops color, but the test line T does not develop color, indicating that the level of chorionic gonadotropin HCG in the cow is low. On the third day, repeat steps 1) and 2) for detection, and the result shows: the test paper quality control line C and the detection line T are all colored, use this color and HCG color card as figure 2 As shown, the following comparisons a...
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