Method for detecting early pregnancy of cow

Abstract

The invention discloses a method for detecting early pregnancy of cow, which comprises the following steps: collecting a sample to be detected, detecting the sample to be detected by using cattle chorionic gonadotropin HCG detection reagent strip, comparing the detection result with a colorimetric card of a kit, and judging the detection result. According to the invention, without urine sample treatment, the operation and detection method is simple, and the method is suitable for on-site, instant and rapid detection; workers can conveniently and accurately determine whether cows are pregnant or not and simply, conveniently, rapidly and accurately detect early pregnancy of cows, whether bovine embryos survive or not can be detected within one week earliest after mating; and furthermore, theeconomic loss caused by false pregnancy and empty pregnancy is further avoided, and false pregnancy and empty pregnancy are eliminated as soon as possible; besides, the cows return to normal as earlyas possible, the non-breeding days of the cows are shortened, the feeding and management mode can be changed in time, the breeding efficiency of the cows is improved, the death rate of the cows afterlactation is reduced, and the breeding benefits of the cows are improved.

Description

technical field [0001] The invention belongs to the technical field of detection, in particular to a method for detecting early pregnancy of cattle. Background technique [0002] Human chorionic gonadotropin (HCG) is a glycoprotein hormone secreted by placental trophoblast cells during pregnancy. It consists of two different subunits, α and β, which are connected by non-covalent bonds. A glycoprotein hormone with a molecular weight of 36700, the α subunit is basically similar to FSH (follicle stimulating hormone), LH (luteinizing hormone) and TSH (thyroid-stimulating hormone) secreted by the pituitary gland, so they can cross-react with each other, while the β subunit The base structure varies. In the process of hormone production, secretion, metabolism, etc., HCG molecules will find various changes such as fragmentation and dissociation, and thus exist in various molecular forms in blood and urine. HCG is the only placental hormone that does not increase with placental we...

AI Technical Summary

Problems solved by technology

However, due to the influence of these hormones in the serum of pregnant and non-pregnant cows due to factors such as breeds and individual differences, it is still difficult to promote them in production.

The erythrocyte agglutination test is to combine the specific antigen in the early pregnancy cow with red blood cells, and use it to prepare antiserum. When mixed with the 10-15 days pregnant cow erythrocytes, erythrocyte agglutination will occur. If not pregnant, erythrocyte agglutination will not occur. , the accuracy of this method is 90% for cows who are pregnant for 28 to 60 days, but the phenomenon of red blood cell agglutination will also occur after the cow embryo stops developing, which will easily cause the cow to be in a false pregnancy state for a long time

Serum acid titration means that during the rapid growth of embryos in cows, the body produces a unique immunoglobulin, which forms low-soluble complexes (floccules) with other proteins in an acidic environment, and the accuracy rate can reach 90.81% %, but after the ewe embryo stops developing, it will also form low-soluble complexes (floccules) with other proteins, and it is also easy to cause the cow to be in a state of false pregnancy for a long time. It can only be carried out in a room temperature environment, which is not conducive to on-site detection