67 results about "Gonadal hormones" patented technology

A compound is provided that has the formula

NH₂CH₂CH₂CHR¹C(O)N—R  (I)

where R¹ is p-chlorophenyl, R is a moiety capable of crossing the blood brain barrier and is as a free compound serotonin, dopamine blood brain barrier (BBB) peptide, membrane translocating protein, TAT peptides, bradykinin, beta-endorphin, bombesin, calcitonin, cholecystokinin, an enkephalin, dynorphin, insulin, gastrin, substance P, neurotensin, glucagon, secretin, somatostatin, motilin, vasopressin, oxytocin, prolactin, thyrotropin, an angiotensin, galanin, neuropeptide Y, thyrotropin-releasing hormone, gonadotropnin-releasing hormone, growth hormone-releasing hormone, luteinizing hormone, vasoactive intestinal peptide transferrin, glucosylamine, amino saccharin, lactylamine, leucine, tryptophan, glutamate and amino cholines.

The present invention relates to Gonadotropin Releasing Hormone (GnRH, also known as Luteinizing Hormone Releasing Hormone) receptor antagonists.

The invention relates to a sperm and ovum health beverage which comprises fulvic acid, gene vitality element, plant extract, fungus polysaccharide, vitamin, hydroxycitric acid (HCA), soluble dietary fiber, L-arginine, L-carnitine, zinc gluconate, calcium lactate, sweetening agent, milk, coffee and purified water. The invention has simple technology, low cost and complete product nutrition and contains rich natural polypeptide, polysaccharide, amino acid and vitamin and various trace elements. All components interact to promote blood circulation and sex hormone secretion so as to achieve the functions of promoting the growth of sperms and ovums, improving the quality and the vitality of the sperms and the ovums and improving the sexual function. The sperm and ovum health beverage has the good improving effect on ED symptoms including renal dysfunction, working strain, heavy spirit pressure, bradypsychia, palpitation, stomachache, weak waist and knee, sexual dysfunction, and the like. As a pure natural preparation, the sperm and ovum health beverage has no poison and side effect and adverse reaction.

Metastin, compounds that promote the activity of metastin or its receptors and the like are excellent gonadal function improving agents, ovulation inducers or promoters, gonadotropic hormone secretion promoters, gonadotropic hormone secretion inhibitors, sex hormone secretion promoters, sex hormone secretion inhibitors, etc., and can be used as agents for preventing/treating sterility, hormone-sensitive cancers, endometriosis, etc. Metastin and its receptors are useful for screening for these pharmaceuticals.

The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.

This invention provides a nucleic acid which encodes a soluble polypeptide which comprises the extracellular domain of a gonadotropin receptor and thioredoxin, wherein the soluble polypeptide is capable of binding to the gonadotropin. This invention also provides the polypeptides encoded by these nucleic acids and methods to produce the polypeptides. This invention also provides methods of preventing or terminating pregnancy, preventing or treating cancer, and decreasing androgen and estrogen production.

The invention discloses a pig oocyte in-vitro maturation culture solution and a preparation method and application thereof, and belongs to the technical field of oocyte in-vitro maturation culture. The problems of low in-vitro maturation rate and development rate of porcine oocytes currently are solved. The pig oocyte in-vitro maturation culture solution is prepared from a basal culture solution TCM-199, penicillin, streptomycin, NaHCO3, 4-hydroxyethylpiperazine ethane sulfonic acid, polyvinyl alcohol, sodium pyruvate, insulin, cysteine, epidermal growth factor, porcine follicular fluid, pregnant mare serum gonadotropin, human chorionic gonadotropin and tannic acid. According to the pig oocyte in vitro maturation culture solution, the oocyte in-vitro maturation quality, the cumulus cell diffusion index, the parthenogenetic embryo blastocyst rate and blastocyst total cell number, and the in-vitro fertilization embryo cleavage rate, blastocyst rate and blastocyst total cell number can beimproved.

A method of preconditioning ovulation induction in a human female comprises of administering an androgen, for example, DHEA, for at least about four consecutive months. DHEA may be administered along with high dose gonadotrophins in ovulation induction treatments. Moreover, DHEA may be administered with follicle stimulating hormone, human menopausal gonadotrophin, norethindrone acetate, leuprolide acetate, and human chorionic gonadotrophin in ovulation induction treatments.

The invention relates to an antagonist of luteinizing hormone releasing hormone (LHRH) containing a hydantoin structure, and particularly relates to a decapeptide derivative which has LHRH receptor antagonism activity, can inhibit hypophysis from secreting gonadotropin and inhibit sexual gland from secreting steroid hormone and is shown in the formula (I): R-Xaa<3>-Ser-Saa <5>-Xaa<6>-Xaa<7>-Xaa<8>-Pro-D-Ala-B, wherein the definition of each symbol is in the specification. The invention also relates to a preparation method of compounds as shown in the formula (1), a pharmaceutical composition containing the compounds and applications of the compounds in preparing medicaments.

The invention discloses a preparation method of a nuisance-free environmentally-friendly pest sticker, and belongs to the technical field of pest stickers. The preparation method of the nuisance-free environmentally-friendly pest sticker adopting natural gum secreted by peaches as the base of the pest sticker comprises the following steps: mixing the natural gum with sodium carbonate, mixing and reacting at a high temperature to increase the viscosity, reacting the obtained product with Vaseline and polybutadiene to form an pest sticker matrix, extracting gonadal hormones and other attractive hormones in pests, and mixing the gonadal hormones and other attractive hormones with the obtained pest sticker to obtain the nuisance-free environmentally-friendly pest sticker. Instances prove that the pest sticker prepared in the invention has the advantages of nontoxic and tasteless property, maintenance of the viscosity in natural state for 1-2 years, and realization of environment pollution due to self degradation after being used, and has a prevention and control effect reaching 85% or above.

The invention discloses a porcine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof and belongs to the technical field of oocyte in-vitro maturation culture. In order to solve the problems of low porcine oocyte in-vitro maturation rate and development rate at present, the invention provides the porcine oocyte in-vitro maturation culture solution aswell as the preparation method and application thereof. The culture solution comprises a base culture solution TCM-199, penicillin, streptomycin, NaHCO3, 4-hydroxyethylpiperazine ethane sulfonic acid, polyvinyl alcohol, sodium pyruvate, insulin, cysteine, an epidermal growth factor, a porcine follicular fluid, pregnant mare serum gonadotropin, human chorionic gonadotropin and ramelteon. The culture solution can increase the oocyte in-vitro maturation quality, the cumulus cell diffusion index, the glutathione level, the parthenogenetic embryo blastocyst rate and blastocyst total cell number, the in-vitro fertilization embryo cleavage rate, the blastocyst rate and blastocyst total cell number and decrease the ROS level of oocytes.

The invention relates to a PVC (polyvinyl chloride) hose which is prepared from the invention relates to a PVC (polyvinyl chloride) hose which is prepared from 100 parts of SG-3 PVC resin, 48-56 parts of non-phthalic plasticizer, 0.9-1.3 parts of Ca/Zn composite stabilizer, 0.1 part of stearic acid, 0-0.02 part of whitener, 0-0.02 part of blue ultramarine, 0-1.5 parts of ACR resin and 0-1.5 parts of titanium white. The PVC hose can not cause environmental pollution since no phthalic plasticizer participates; and the disorder of gonadal hormones in the human body causes disorder of sexuality of the human body.

The invention relates to application of clomifene citrate to anti-mycobacterium tuberculosis medicines. Clomifene citrate has estrogenic and antiestrogenic properties that appear to prevent the release of gonadotropins, follicle-stimulating hormone and luteinizing hormone, thus leading to follicular development and maturation, ovulation and subsequent corpus luteum development and function and then resulting in pregnancy. It is found through researches that the clomifene citrate has an antitubercular effect and has good development value. The application of the clomifene citrate to anti-mycobacterium tuberculosis medicines and the obvious anti-mycobacterium tuberculosis effect of the clomifene citrate are disclosed for the first time.

The invention belongs to a cell biology technology, and particularly relates to an extraction and culture method of mouse ovarian granular cells. According to the technical scheme, the method comprises the following specific steps: (1) injecting pregnant mare serum gonadotropin PMSG into the abdominal cavity of a female mouse; (2) killing the mouse by using a cervical vertebra dislocation method, separating the ovary of the mouse, cleaning with PBS (Phosphate Buffer Solution), and removing a fat envelope around the mouse under a microscope; (3) puncturing follicles in the mixed operation solution by using a syringe needle to release granular cells and oocytes, filtering by using a screen, centrifuging the filtrate, and discarding the supernatant; and (4) mixing the complete culture medium, resuspending the cells, precipitating and inoculating, wherein the operation liquid includes DME/F-12:1(1X), 3% of fetal calf serum, 2% of mycillin mixed liquid and 2% of mycoplasma scavenger. Furthermore, the mouse is an immature female mouse which is 3 weeks old and 10-13g in weight, the injection amount of the PMSG is 5-10IU, the injection method is intraperitoneal injection, the injection amount of the PMSG is preferably 8IU, and the time for killing the mouse after cervical vertebra dislocation is 46-48h after injection. The mechanical method provided by the invention is simple and convenient to operate and short in time consumption, and the obtained ovarian granular cells are high in yield.

The invention discloses a ten-medicine Xiang Lu, a preparation method thereof and a ginsenoside content analysis method, and belongs to the technical field of traditional Chinese medicine formulas. The capsule gives full play to the values of the traditional Chinese medicines, has the effects of soothing liver-qi stagnation, softening hardness to dissipate stagnation and the like, the pharmacodynamic material basis of ginseng is ginsenoside which has an adjusting effect on the neuroendocrine function of the pituitary-gonad axis and can excite the pituitary to secrete gonadotropin, promote biosynthesis of DNA and protein synthesis related zymoprotein and increase the gonad activity. Through the two-way regulation effect on the pituitary-gonad axis, the positive and negative feedback functions of neuroendocrine are balanced so as to achieve the purpose of reducing estradiol which is abnormally increased, and mammary gland cell proliferation controlled by ovarian hormone is inhibited; andinternal components of traditional Chinese medicines are selected as internal standards in a quantitative analysis of multiple components by single marker, and the content of the to-be-detected components is calculated by measuring relative correction factors (RCF) between the internal standards and other to-be-detected components, so that the use amount of reference substances is reduced, and the cost is saved.

The invention discloses a porcine oocyte in-vitro maturation system containing butoxylic acid and an application of the porcine oocyte in-vitro maturation system. A culture solution is prepared from a basic culture solution TCM-199, cysteine, penicillin, streptomycin, an insulin-like growth factor, an epidermal growth factor, fetal calf serum, pig follicle fluid, NaHCO3, pregnant mare serum gonadotropin, human chorionic gonadotropin and Bufexamac, and the apoptosis rate of in-vitro development of oocytes can be reduced; and the first polar body discharge rate, the parthenogenetic embryo cleavage rate, the blastocyst rate, the blastocyst total cell number, the in-vitro fertilization embryo cleavage rate, the blastocyst rate and the blastocyst total cell number are improved.

The invention discloses crystal forms and an amorphous form of a gonadotropin releasing hormone (GnRH) receptor antagonist drug relugolix and a preparation method of the crystal forms and the amorphous form, and relates to the field of chemical medicines. The crystal forms and the amorphous form provided by the invention have good stability, and have great significance for the purification processof active pharmaceutical ingredients and the preparation process of drugs.

The inventors have discovered that substituting amino acid residues at positions 5 and/or 7 and/or 8 of the GnRH I (gonadotropin-releasing hormone) peptide sequence, and particularly removal of the arginine residue at position 8, results in elevated antiproliferative activitey. Furthermore, the introduction of certain D-amino acid residues at position 6 of the GnRH II peptide sequence results in elevated antiproliferative activity. This surprising discovery provides potent and selective agents for use in treating antiproliferative disorders, such as cancer.