79 results about "Chorionic gonadotropins" patented technology

The invention provides a method for preparing follicle-stimulating hormone FSH. The method comprises the steps as follows: (A) a raw material containing the FSH is contacted with an antibody, wherein, the raw material contains luteinizing hormone LH and/or chorionic gonadotropin CG as impurities, and the antibody is an antibody which specifically combines the LH and/or the CG but not crossly react with the FSH basically, thereby making the antibody form an antigen-antibody compound with the LH and/or the CG; (B) the formed antigen-antibody compound is removed from the raw material, thereby obtaining purified FSH. The invention also provides a method for preparing a drug composition containing the FSH.

An artificial reproduction method for lateen mussel or butterfly mussel includes such steps as choosing more than 3 years old parents, temporarily culturing in adult mussel pool, injecting the chorionic gonadotropin in female parent, culturing the female parents in individual pool, taking the seminal fluid from male parent, diluting by sodium chloride solution, injecting it in female parent twice, culturing in temporary pool until glochidium is mature, parasitizing and culturing young mussel.

The invention provides a method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination, which relates to the artificial propagation of gobies. The method required tobe provided for artificially breeding the tridentiger trigonocephalus comprises the steps of ripe fish screening, maturity acceleration, insemination, incubation and larval fish culture, wherein the maturity acceleration of ripe fishes uses luteotropin to release hormone analogues, domperidone maleate and chorionic gonadotropin, and is characterized in that the three hormones are used twice for the maturity acceleration; in the maturity acceleration, the water temperature of culture is between 18 and 21 DEG C, the salinity is between 2 and 6, the dissolved oxygen is between 3.5 and 6.5mg/L, and the pH is between 7.5 and 9.0; and after the second time of the maturity acceleration, in 8 to 10 hours, mature ova and mature sperms are selected for artificial insemination which comprises the following steps that: the activated sperms are poured on the ova for sufficient contact so as to obtain fertilized ova; the fertilized ova are placed in a hatchery pond for incubation; and the hatched larval fish is placed in a culture pond for incubation, and chlorella and yolk powder of eggs are placed in water from the third day after the larval fish is hatched to feed food for the larval fish. The method is used for the artificial breeding of the tridentiger trigonocephalus.

The invention relates to a method for inducing hormone soaked loaches. The method comprises the following steps: (1) selecting a mature loach parent in water at the temperature of between 21 and 29 DEG C; and (2) taking a mixture of domperidone maleate, luteotropin releasing hormone d-ala analog and chorionic gonadotropin as oxytocic hormones, and soaking the loach parent with a soaking solution which is prepared by mixing the oxytocic hormone, pentoxifylline and edible oil in normal saline, wherein the dosage of the domperidone maleate is between 100 and 300mg/kg, the dosage of the luteotropin releasing hormone d-ala analog is between 200 and 600mu g/kg, the dosage of the chorionic gonadotropin is between 15,000 and 30,000IU/kg, and the dosage of the normal saline is between 0.2 and 0.3kg/kg; and when the loach parent is soaked, the temperature of the soaking solution is controlled to be between 21 and 29 DEG C, and the soaking time is controlled to be between 0.5 and 8 hours. The aim of inducing the loach parent can be realized through soaking instead of injection.

The invention relates to a complete artificial propagation method for acrossocheilus fasciatus. The method comprises: acquiring wild acrossocheilus fasciatus juvenile fishes for acclimation, culturing until sex matured, using exogenous parturifacient chorionic gonadotropin (HCG), lutropin releasing hormone analog (LHRH-A3) and domperidone (DOM), through a one-time injection method, to promote sexual gland of parent fishes of acrossocheilus fasciatus which is mature naturally to develop further, through dry insemination to obtain oosperms, and through a special hatching equipment, performing flowing hatching until hatching. Rate of inducing spawning of parent fishes reaches up to 85%, and fertility rate reaches above 85%, and hatchability is above 70%. The method solves problems of large-scale production of acrossocheilus fasciatus fries.

The invention discloses a high survival rate loach drug spawning technology, and relates to an artificial spawning technology. The technology concretely comprises the following steps: fixing a spawning period, fixing male and female ratio, preparing and spraying drug soup, performing prevention before spawning and injecting odinagogue; the time is 15 to 20 hours; water temperature is fixed to be 20 to 25 DEG C; loaches are wrapped through towels; 0.1 to 0.2 milliliters of chorionic gonadotropin and Ringer's solution blending agent is injected to each loach, wherein the chorionic gonadotropin occupies 75 percent, and the Ringer's solution blending agent occupies 25 percent. The technology has the characteristics that the loaches absorb the drug soup before delivery, so that the quality of seminal fluid and egg is improved, prevention mixture properly protects the loaches, the survival amount of produced loach fries is high, the yield is high, the method is unique and simple and has good effect, and breeding with the technology is easy to promote for households.

The present invention is directed to methods of treating a chronic pelvic pain syndrome comprising the step of administering to a subject suffering from prostatis a chorionic gonadotropin or a pharmaceutically active fragment or derivative thereof in an amount effective to alleviate one or more symptoms of a chronic pelvic pain syndrome.

The invention provides a recombinant pig FSH-CTP fusion protein. The fusion protein means that a beta subunit of pig FSH is directly or indirectly linked to a beta subunit carboxy terminal peptide CTPof human, primate or equine mammalian chorionic gonadotropin, and an alpha subunit of pig FSH is bound to the beta subunit of pig FSH through Van der Waals force. The fusion protein can be prepared by using a eukaryotic expression system on the basis of a genetic engineering technology. Compared with pig pituitary FSH, the pig FSH-CTP fusion protein provided by the invention has a longer half-life and a better drug effect, and can replace pig pituitary FSH and pregnant horse serum gonadotropin in the current market in the field of animal propagation.

The invention discloses a breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda. The method includes the following steps: 1) selecting selectively-bred erythroculter ilishaeformis and selectively-bred ancherythropterus nigrocauda as parents; 2) conducting parents strengthening breeding including erythroculter ilishaeformis strengthening breeding and ancherythropterus nigrocauda strengthening breeding; 3) conducting artificial spawning induction: respectively injecting spawning induction medicine hypophysis cerebri or dopamine antagonist DOM, lutein releasing hormone analogue and chorionic gonadotropin which are used singly or in mixing mode on the two parents; 4) conducting artificial semen and spawn collection and insemination including dry method insemination and semi-dry method insemination; and 5) conducting artificial incubation. Hybridized culter 'vanguard 1' obtained by distant hybridization has the advantages of good body type and large size of the erythroculter ilishaeformis, also has the advantages of low breeding cost, mild temper, easiness in catching and easiness in live marketing of the ancherythropterus nigrocauda and further has excellent hybridization performances like high growing speed.

The invention relates to antibody characteristics used to design a whole blood Point of Care Thyroid Stimulating Hormone (TSH) immunoassay using an ELISA sandwich assay lacking one or more wash steps between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).

Provided is a test paper for testing chorionic gonadotropin in human saliva. The test paper comprises a primary sample pad, nitrocellulose filter membrane with testing T line and quality control C line, a sample suction pad and a bottom plate. The primary sample pad, the nitrocellulose filter membrane and the sample suction pad are respectively connected with the bottom plate through lap joints to form a test paper; among which the upper side of the primary sample pad is pasted with a secondary sample pad for filtering the impurities in the sample. The test paper has the advantages of 1, the design of the secondary sample pad in both single and double channel test papers can effectively filter the impurities in the saliva, especially the mucoprotein, miscellaneous bacteria and the like; 2, the sample in the double channel test paper passes through a first level channel to touch the test line without creating any composite substance through the combination with labeled antibody in the binding pad, the labeled antibody passes through a second level channel and touches the test line. This test paper overcomes the concentration/coagulation problem between the labeled sample and sample analyte.

A method for promoting the sex gland of parent stumpnose bream to be mature and ovulation includes such steps as culturing the parent stumpnose bream in the netted case in nutritive sea region, culturing them in a circulated filter-type reproduction pool when the cocycte is greater than 400 microns in diameter, and injecting synthetic luriberin (LHRH-A2) and chorionic gonadotropin (HCG).

The invention discloses an artificial-inducing mixing agent and artificial breeding method for largemouth bass, and belongs to the technical field of aquatic animal breeding. The provided mixing agentincludes chorionic gonadotropin, luteinizing hormone releasing hormone analogue No.2 and DOM. Through the adoption of the mixing agent to artificially breed largemouth bass, bred females can have high brood amount, high egg maturity can be achieved, fertilization rate and hatchability can be significantly increased, the breeding efficiency of the largemouth bass can be effectively enhanced, and time can be saved.

The invention discloses an artificial selecting-breeding method of antibacterial-disease good varieties of bostrichthys sinensis. The artificial selecting-breeding method includes the specific steps that survival fish fingerling entities after bacterial diseases are collected in high disease-affecting season; chorionic gonadotropin and luteinizing hormone-releasing hormone are injected into female fishes before parent fishes lay eggs, and chorionic gonadotropin is injected into male fishes; fertilized eggs are incubated at the temperature of 20 DEG C to 30 DEG C, and the antibacterial-disease good varieties of the bostrichthys sinensis are obtained. The artificial selecting-breeding method has the advantages that the survival fish fingerling entities after bacterial diseases in high disease-affecting season are selected as the parent fishes, and the antibacterial-disease capacity is high; the problem that fry of the bostrichthys sinensis are short is solved, large-scale breeding of the fry of the bostrichthys sinensis is achieved, the requirements of farmers for artificial fry of the bostrichthys sinensis are met, and the artificial selecting-breeding method is suitable for application and popularization of bostrichthys-sinensis breeding farms in national coastal areas, and has the large development market space.

The invention discloses a culture solution for promoting in-vitro maturation of porcine oocyte, the culture solution takes an M199 culture solution without HEPES as a basic culture solution, and porcine follicular fluid, L-cysteine, sodium pyruvate, epidermal growth factor, insulin, gonadotropin, chorionic gonadotropin and WNT5A cytokine are also added. When the culture solution is used for carrying out in-vitro culture on the porcine oocytes, the in-vitro maturation efficiency and quality of the oocytes can be effectively improved, the obtained mature oocytes are used for carrying out porcinein-vitro fertilization and somatic cell nuclear transfer embryo production, and the blastocyst development rate and quality of the mature oocytes are also remarkably improved.

The invention relates to an injection oxytocin for loach. The oxytocin is mixed from three hormones of DOM, LRHA and chorionic gonadotropin, wherein an amount of the DOM is 45-55mg/kg; an amount of the LRHA is 90-110[mu]g/kg, and an amount of the chorionic gonadotropin is 7000-8000IU/kg. According to the invention, the three hormones are reasonably proportioned to realize an objective of low usage amount and high induction efficiency.

The invention relates to immunotherapy of a mammalian subject by exposing the immune response cells of the subject to a nucleic acid construct encoding at least one hCG immunogenic epitope or precursor thereof such that the nucleic acid construct is taken up and processed by the immune response cells. The invention further relates to compositions comprising such hCG-encoding nucleic acid constructs.

The invention discloses a rapid propagation method for mud fish. The method comprises the following steps of (1) selecting the mud fish which are two to three-winter-old, 15 to 20cm in body length and 30 to 50 grams in body weight as propagation parents, and ensuring that the female/male ratio of the parent mud fish is 1:2-1:3; (2) taking the parent mud fish out of a culture pond, putting the parent mud fish on a spawning induction bed, and injecting 800 to 1,000 international units of chorionic gonadotropin to each parent female mud fish and 400 to 500 international units of chorionic gonadotropin to each parent male mud fish by adopting an intramuscular injection method; (3) putting the parent mud fish subjected to spawning induction into an incubation jar for spawning, taking the parent mud fish in a net of the incubation jar out after 20 hours of spawning, ensuring that eggs are independently incubated in the incubation jar, continuously injecting new clear water in the incubation process to ensure that water in the incubation jar flows; (4) after incubating for 1 month, making sure that fries of the mud fish in the length of 2.5-3.6cm are formed. According to the method, the propagation time of the mud fish is greatly shortened, the hatching rate is improved, the manpower is reduced, and the aquaculture economic benefit is improved.

A method of controlling, in a warm-blooded animal, an infectious microorganism expressing a chorionic gonadotropin-like growth-regulating protein comprises modulating the activity of the protein by administering to the warm-blooded animal an effective amount of an antibody capable of inhibiting the activity of the protein, the chorionic gonadotropin-like growth-regulating protein itself such that the animal develops a neutralizing immune response, or peptides that block the receptors of the chorionic gonadotropin-like growth-regulating protein in the infectious microorganism. Candida albicans is one such microorganism that can be controlled by delivering an antibody against the growth-regulating protein, CaCGLP. Mycobacteria can also be controlled by this method. A method of inhibiting transition of Candida albicans blastospores in vitro by contacting the blastospores with an antibody against CaCGLP is also disclosed. A method of inhibiting growth of mycobacteria in vitro comprises contacting the mycobacteria with an antibody against an endogenous chorionic gonadotropin-like growth-regulating protein. In vitro growth of microorganisms can also be stimulated by contacting the microorganism with a chorionic gonadotropin-like growth-regulating protein.

The invention relates to an artificial induced spawning technology, in particular to an efficient loach artificial fertilization technology. The efficient loach artificial fertilization technology particularly comprises the following steps of determining the induced spawning date, determining the proportion of males and females, preparing and spraying medicine soup, carrying out prevention before induced spawning and injecting odinagogue, wherein the time is 15-20 hours, the water temperature is determined to range from 20 DEG C to 25 DEG C, loach is wrapped with a towel, a mixture of 0.1-0.2 milliliter is injected to each piece of loach, and the mixture includes 75% of chorionic gonadotropin and 25% of a Ringer solution. The efficient loach artificial fertilization technology has the advantages that the loach absorbs the medicine soup before induced spawning is carried out, so that the quality of seminal fluid and eggs is improved, the loach is better protected through a prevention mixture, the amount of survival young loach is large, yield is high, and the efficient loach artificial fertilization technology is unique, simple, good in effect and easy to popularize in family cultivation.