Method for inducing hormone soaked loaches

A hormone and loach technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of large parent damage, large fish stimulation, and impact, saving time for catching and injection, and reducing trauma Effect

Inactive Publication Date: 2011-09-14
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development potential of loach farming is great, but the number of natural seedlings of loach can no longer effectively supply the breeding needs, which restricts the development of loach farming industry
[0003] The traditional method of injecting oxytocin will encounter the problem that loach is difficult to catch
In the artificial breeding process, due to the small size of the loach, it takes a lot of time to catch and inject one by one, which affects the damage of the parents.
[0004] Chinese patent CN200710114040.9 discloses a slow-release hormone granule that induces the maturation of the gonads of the stone seabream and its preparation method, which solves the problems of troublesome operation and great stimulation to the fish body existing in the method of direct injection of the hormone
However, it is also by injecting liquid medicine into the body of the parent, so that the liquid medicine becomes solid in the body of the parent and released slowly so as to achieve long-term release of the drug effect, which will cause greater damage to the parent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The induced labor method of hormone-soaked loach comprises the following steps:

[0025] ①Choose 23 kg of mature loach parents and place them in 21°C water;

[0026] ②A mixture of dioconone maleate, luteinizing hormone-releasing hormone analog and chorionic gonadotropin is used as the oxytocin hormone, and the oxytocin hormone, toxifylline, and edible oil are mixed in physiological saline to make soaking Loach parents were soaked in liquid; wherein, the dosage of dioconone maleate was 100mg / kg, the dosage of luteinizing hormone releasing hormone analogue was 200μg / kg, the dosage of chorionic gonadotropin was 15000IU / kg, and the dosage of normal saline was 0.2kg / kg. kg, the dosage of toxifylline is 180 mg / L, and the dosage of edible oil is 3mg / L; when soaking, the temperature of the soaking solution is controlled at 21°C, and the soaking time is controlled at 8 hours. The induced labor rate was 37%.

Embodiment 2

[0028] The induced labor method of hormone-soaked loach comprises the following steps:

[0029] ①Choose 25 kg of mature loach parents and place them in 22°C water;

[0030] ②A mixture of dioconone maleate, luteinizing hormone-releasing hormone analog and chorionic gonadotropin is used as the oxytocin hormone, and the oxytocin hormone, toxifylline, and edible oil are mixed in physiological saline to make soaking Loach parents were soaked in liquid; wherein, the dosage of dioconone maleate was 120mg / kg, the dosage of luteinizing hormone releasing hormone analogue was 225μg / kg, the dosage of chorionic gonadotropin was 15500IU / kg, and the dosage of normal saline was 0.2kg / kg. kg, the dosage of toxifylline is 180 mg / L, and the dosage of edible oil is 3mg / L; when soaking, the temperature of the soaking solution is controlled at 22°C, and the soaking time is controlled at 7 hours. The induced labor rate was 54%.

Embodiment 3

[0032] The induced labor method of hormone-soaked loach comprises the following steps:

[0033] ①Choose 25 kg of mature loach parents and place them in 23°C water;

[0034] ②A mixture of dioconone maleate, luteinizing hormone-releasing hormone analog and chorionic gonadotropin is used as the oxytocin hormone, and the oxytocin hormone, toxifylline, and edible oil are mixed in physiological saline to make soaking Soak the parents of loach in liquid; wherein, the dosage of dioconone maleate is 145mg / kg, the dosage of luteinizing hormone releasing hormone analogue is 250μg / kg, the dosage of chorionic gonadotropin is 15750IU / kg, and the dosage of normal saline is 0.2kg / kg. kg, the dosage of toxifylline is 200 mg / L, and the dosage of edible oil is 4mg / L; when soaking, the temperature of the soaking solution is controlled at 23°C, and the soaking time is controlled at 6 hours. The induced labor rate was 69%.

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PUM

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Abstract

The invention relates to a method for inducing hormone soaked loaches. The method comprises the following steps: (1) selecting a mature loach parent in water at the temperature of between 21 and 29 DEG C; and (2) taking a mixture of domperidone maleate, luteotropin releasing hormone d-ala analog and chorionic gonadotropin as oxytocic hormones, and soaking the loach parent with a soaking solution which is prepared by mixing the oxytocic hormone, pentoxifylline and edible oil in normal saline, wherein the dosage of the domperidone maleate is between 100 and 300mg/kg, the dosage of the luteotropin releasing hormone d-ala analog is between 200 and 600mu g/kg, the dosage of the chorionic gonadotropin is between 15,000 and 30,000IU/kg, and the dosage of the normal saline is between 0.2 and 0.3kg/kg; and when the loach parent is soaked, the temperature of the soaking solution is controlled to be between 21 and 29 DEG C, and the soaking time is controlled to be between 0.5 and 8 hours. The aim of inducing the loach parent can be realized through soaking instead of injection.

Description

technical field [0001] The invention relates to a method for inducing labor of loach, in particular to a method for inducing labor of loach soaked in hormones. Background technique [0002] Loach (Misgurnus arguillicaudatus) belongs to Cyprinidae, Loach family, Loach genus, commonly known as "pink loach". It has a wide distribution and strong adaptability, and is an omnivorous small freshwater economic fish. Loach meat is delicious, has high nutritional value and medicinal value, and is deeply loved by consumers at home and abroad. Loach farming has great development potential, but the number of natural seedlings of loach can no longer effectively supply the breeding needs, which restricts the development of loach farming industry. [0003] The traditional method of injecting oxytocin will encounter the problem that loaches are difficult to catch. In the artificial breeding process, due to the small size of the loach, it takes a lot of time to catch and inject one by one,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 胡廷尖刘士力王雨辰周志明李倩黄小红
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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