The invention discloses a
protein isoelectric focusing electrophoresis method and glue preparation device, and belongs to the technical field of bioseparation detection. The method comprises the stepsof glue preparation, glue filling, pre-
electrophoresis,
electrophoresis, gel fixing, decoloration,
dyeing and the like. According to the method, the
feeding mode of a sample and
voltage and electrophoresis time of pre-electrophoresis and electrophoresis are changed, so that obtained
protein electrophoresis bands are clear, good in separation degree and do not diffuse or trail, the problem that inthe prior art, the electrophoresis time is long, and
protein diffuses and trails is effectively solved, and the glue preparation success rate is greatly increased. The glue preparation device comprises a glue preparation support, a
fixed frame, glue preparation glass, a
gasket, an anti-leakage pad and other components. By improving the structure of the glue preparation device, the consumption ofa
reagent is reduced, glue filling is fast, no bubble is generated, glue gathering is even, the glue preparation success rate is greatly increased, and meanwhile the inspection cost is reduced.