Hydrogel compound, preparation method and application
A composite and hydrogel technology, which is applied in the field of biomedical engineering, can solve the problems of poor matrix integration, poorer performance than hyaline cartilage, and short cell life, and achieve the effects of rapid gelation, increased in vivo stability, and less trauma
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[0067] Preparation of aldehyde modified hyaluronic acid
[0068] The aldehyde-modified hyaluronic acid of the present invention can be prepared by the following two methods:
[0069] The first method: oxidizing hyaluronic acid to open the ring to obtain an aldehyde group, thereby obtaining an aldehyde group-modified hyaluronic acid (I). Add the oxidant to the hyaluronic acid solution (HA) according to the molar ratio of HA to oxidant (1:1) to (1:10), and place the system in the dark to react for 0.5-6 hours. Ethylene glycol was added to remove excess oxidant, and after stirring for 1 hour, the product was recovered, purified and dried to obtain aldehyde-modified hyaluronic acid (I). The oxidant may be one of periodate, permanganate, chlorate and the like.
[0070] The second method:
[0071] According to the first method mentioned above, the aldehyde-modified hyaluronic acid (I) is modified on the basis of the aldehyde-modified hyaluronic acid (I) and benzaldehyde groups are intro...
Example Embodiment
[0082] Example 1
[0083] Use sodium hyaluronate with a molecular weight of 100,000 to 200,000 daltons to be dissolved in water at room temperature to form a 0.01g / mL hyaluronic acid solution. Add cation exchange resin and stir overnight, filter to obtain hyaluronic acid solution (HA). To 100mL of 0.01g / mL hyaluronic acid solution, 5mL of 0.5M sodium periodate solution was added for oxidation, and the system was placed in the dark to react for 3 hours. Add 0.5 mL of ethylene glycol to remove excess sodium periodate. After stirring for 1 hour, the product was collected, and the product was dialyzed for three days with a dialysis bag with a molecular weight cut-off of 3500 Dalton, and lyophilized to obtain aldehyde-modified hyaluronic acid (HA- A). The degree of substitution of aldehyde groups is 37% (such as figure 2 Shown).
Example Embodiment
[0084] Example 2
[0085] Use sodium hyaluronate with a molecular weight of 100,000 to 200,000 daltons to be dissolved in water at room temperature to form a 0.03g / mL hyaluronic acid solution. Add cation exchange resin and stir overnight, filter to obtain hyaluronic acid solution (HA). To 100mL of 0.03g / mL hyaluronic acid solution, 15mL of 5M sodium periodate solution was added for oxidation, and the system was placed in the dark to react for 0.5 hours. Add 10 mL of ethylene glycol to remove excess sodium periodate. After stirring for 1 hour, the product was collected. The product was dialyzed against a dialysis bag with a molecular weight cut-off of 3500 Dalton for three days, and lyophilized to obtain aldehyde-modified hyaluronic acid (HA-A ).
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