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Test paper for testing chorionic gonadotropin in human saliva and preparation method thereof

A chorionic gonadotropic and human detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as interference sensitivity, achieve high sensitivity of false positive rate, improve good labeling, and enhance the effect of detection signals

Inactive Publication Date: 2017-02-01
武汉百美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, urine HCG test paper mostly uses colloidal gold immune double-antibody sandwich method. This kind of HCG colloidal gold immunochromatographic test paper cannot reach this level, especially in terms of saliva non-detection substance interference and sensitivity.

Method used

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  • Test paper for testing chorionic gonadotropin in human saliva and preparation method thereof
  • Test paper for testing chorionic gonadotropin in human saliva and preparation method thereof
  • Test paper for testing chorionic gonadotropin in human saliva and preparation method thereof

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preparation example Construction

[0051] Another aspect of the present invention provides the preparation method of the first aspect test paper, comprising the steps of:

[0052] S1: Preparation of sample pads

[0053] Preparation of the first sample pad 1: place the cut glass fiber membrane, or non-woven fabric, or filter test paper in the treatment liquid of the first sample pad 1, soak at room temperature, dry, and set aside;

[0054] Further, the preparation method of the first sample pad 1 treatment solution is as follows: weigh 0.5g±0.05g BSA into a beaker, add 70ml of 10mM pH8.4 borate buffer solution to dissolve, add Tween-20, mix well, Constant volume, filter membrane and set aside.

[0055] The preparation of the second sample pad 5: choose filter test paper with a pore size of 500nm-1000um, or glass fiber membrane, or non-woven fabric, or water-absorbing filter paper, soak and dry in a hydroxyapatite liquid with a concentration of 0.1-100mg / ml at room temperature dry, spare;

[0056] Further, the...

Embodiment 1

[0069] Preparation of human salivary chorionic gonadotropin (HCG) rapid immunodiagnostic test paper

[0070] 1. The required raw materials are as follows:

[0071] 1.1 Reagents

[0072] Anti-α-HCG and β-HCG antibodies, cellulose nanoparticles (cellulose nanoparticles produced by Asahi Kasei, diameter 340±20mm), casein, hydroxyapatite, trehalose, NaCl, Na2HPO4·12H2O, NaH2PO4· 2H2O, boric acid, borax, Tween-20, Triton X-100, sucrose, ultrapure water, etc.;

[0073] 1.2 Consumables

[0074] Nitrocellulose membrane (NC membrane), 8975 glass fiber, GF-06 glass fiber, absorbent pad, bottom plate 4, volumetric flask, Erlenmeyer flask, EP tube, pipette tip;

[0075] 1.3 Solution preparation

[0076] Preparation of PBS buffer solution: Weigh 1.615-2g of Na2HPO4·12H2O, 0.225-5g of NaH2PO4·2H2O, and 4.0-5.0g of NaCl into a beaker, add 400ml of ultrapure water to dissolve to 500ml, filter through a 0.22μm filter membrane and set aside;

[0077] Preparation of 0.2M boric acid solution...

Embodiment 2

[0101] Sensitivity test of human salivary chorionic gonadotropin (HCG) rapid immunodiagnostic test strip

[0102] 1. Saliva collection: collect by saliva swabbing method, collect saliva and put it into saliva diluent, and rub both sides of the collection stick on the tube wall filled with saliva diluent for 5-6 times.

[0103] 2. Spiked saliva: Take 98 μL of saliva in the microwell, add 2.5 μL m IU / ml HCG standard to get the saliva spiked with 25 mIU / ml; take 98 μL and 95 μL of saliva in the microwell, add 2 μL and 5 μL 100mIU / ml HCG standard can be spiked with 2mIU / ml, 5mIU / ml saliva;

[0104] 3. Test: Put the dual-channel test strip on the table, take 50 μL of saliva and drop it on the second sample pad 5, and let it stand at room temperature for 1-5 minutes. After the color of the suction pad 3 changes, take 50 μL of LPBS buffer solution Put it on the binding pad 6 drop by drop, let it stand at room temperature for 5 minutes, observe the results, the sensitivity of saliva ...

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Abstract

Provided is a test paper for testing chorionic gonadotropin in human saliva. The test paper comprises a primary sample pad, nitrocellulose filter membrane with testing T line and quality control C line, a sample suction pad and a bottom plate. The primary sample pad, the nitrocellulose filter membrane and the sample suction pad are respectively connected with the bottom plate through lap joints to form a test paper; among which the upper side of the primary sample pad is pasted with a secondary sample pad for filtering the impurities in the sample. The test paper has the advantages of 1, the design of the secondary sample pad in both single and double channel test papers can effectively filter the impurities in the saliva, especially the mucoprotein, miscellaneous bacteria and the like; 2, the sample in the double channel test paper passes through a first level channel to touch the test line without creating any composite substance through the combination with labeled antibody in the binding pad, the labeled antibody passes through a second level channel and touches the test line. This test paper overcomes the concentration / coagulation problem between the labeled sample and sample analyte.

Description

technical field [0001] The invention relates to the technical field of in vitro immunodiagnostic reagents, in particular to a test paper for detecting chorionic gonadotropin in human saliva and a preparation method thereof. Background technique [0002] When women of childbearing age are pregnant, placental trophoblast cells secrete a hormone called human chorionic gonadotropin (HCG). It is a glycoprotein hormone composed of α and β subunits, in which the α subunit is basically similar to follicle stimulating hormone (FSH), luteinizing hormone (LH) and thyroid stimulating hormone (TSH) secreted by the pituitary gland, so They can cross-react with each other, and the structures of the β subunits are different. The main function of HCG is to stimulate the corpus luteum, which is conducive to the continuous secretion of estrogen and progesterone, so as to promote the formation of decidua and make the placenta grow and mature. In mature women, after the fertilized egg moves to...

Claims

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Application Information

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IPC IPC(8): G01N33/76G01N33/558
CPCG01N33/76G01N33/558G01N2800/36
Inventor 马北峰马北阳徐晓峰
Owner 武汉百美生物科技有限公司
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