Artificial breeding technology for Oriental loaches
An artificial breeding and plateau technology, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of artificial breeding technology reports of oriental plateau loach
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Embodiment 1
[0028] experimental method
[0029] The parents of Oriental Plateau Loach were captured from the Yarlung Zangbo River and transported to the aquaculture base of Tibet Agriculture and Animal Husbandry College in Linzhi, where they were disinfected with 10 mg / L potassium permanganate for 10 minutes.
[0030] Select 20 individuals with large females, soft and elastic abdomen, red and swollen genital pores, body length of 12cm, and weight of 13g or more; select males that are larger, with obvious secondary sexual characteristics in the muzzle and pectoral fins, thick pads and spines , And 10 individuals with a body length of 10 cm and a weight of 11 g or more were kept temporarily in a cage of 0.6 × 0.5 × 0.6 m.
[0031] Choose chorionic gonadotropin (HCG), luteinizing hormone releasing hormone analog A 2 (LHRH-A 2 ) And dioscillate (DOM), the female fish's inducing dose is HCG2000IU / kg, LHRH-A 2 25μg / kg, DOM6mg / kg, male fish dose halved.
[0032] Begin artificial induction of labor. Befo...
Embodiment 2
[0042] experimental method
[0043] The parents of Oriental Plateau Loach were captured from the Yarlung Zangbo River and transported to the aquaculture base of Tibet Agriculture and Animal Husbandry College in Linzhi, where they were disinfected with 10 mg / L potassium permanganate for 10 minutes.
[0044] Select 20 individuals with large females, soft and elastic abdomen, red and swollen genital pores, body length of 12cm, and weight of 13g or more; select males that are larger, with obvious secondary sexual characteristics in the muzzle and pectoral fins, thick pads and spines , 10 individuals with a body length of 10 cm and a weight of 11 g or more were kept temporarily in a cage of 0.6×0.5×0.6m.
[0045] The oxytocin drug chooses chorionic gonadotropin (HCG), luteinizing hormone releasing hormone analog A 2 (LHRH-A 2 ) And domenone maleate (DOM), the female fish inducing dose is HCG2500IU / kg, LHRH-A 2 30μg / kg, DOM8mg / kg, male fish dose halved.
[0046] Begin artificial induction o...
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