Artificial-inducing mixing agent and artificial breeding method for largemouth bass
A technology of artificial induced labor and artificial breeding, applied in fish farming, drug combination, medical formula, etc., can solve the problems of low egg fertilization and hatching rate, insufficient nutrient supply, poor survival rate of seedlings, etc., so as to improve the quality of eggs , Reduce egg atresia and overripe phenomenon, reduce the effect of occurrence probability
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Embodiment 1
[0047] A set of improved techniques for the cultivation and artificial breeding of largemouth bass broodstock comprises the following steps:
[0048] Step 1. One month before broodstock breeding, select male and female broodstock with normal body shape and color, strong physique, and no disease or disability. Put the selected broodstock into running water with a water temperature of 24°C and a water flow rate of 15L / min. During the cultivation period, the protein level was 44%, and the feed oil level was 10% (fish oil: soybean oil: coconut oil according to the ratio of 60%: 20%: 20%) was fed twice a day. Every 2 days, spray 100mg of vitamin E+vitamin A mixture (1:2) per kilogram of feed to feed broodstock once to accelerate gonad development and maturation. At the same time, conventionally cultured largemouth bass broodstock were selected as the control group, and fed with California perch special feed produced by Zhejiang Xinxin Tianen Aquatic Feed Co., Ltd., with a crude pr...
Embodiment 2
[0061] A set of improved techniques for the cultivation and artificial breeding of largemouth bass broodstock comprises the following steps:
[0062] Step 1. One month before broodstock breeding, select male and female broodstock with normal body shape and color, strong physique, and no disease or disability. Put the selected broodstock into running water with a water temperature of 22°C and a water flow rate of 10 L / min. During the cultivation period, the protein level was 45%, and the feed oil level was 9% (fish oil: soybean oil: coconut oil according to the ratio of 50%: 20%: 30%) twice a day. Every 2 days, spray 100mg of vitamin E+vitamin A mixture (1:2) per kilogram of feed to feed broodstock once to accelerate gonad development and maturation. At the same time, conventionally cultured largemouth bass broodstock were selected as the control group. At the same time, conventionally cultured largemouth bass broodstock were selected as the control group, and fed with Califo...
Embodiment 3
[0078] In order to explore the effect of the oxytocic mixture that utilizes the compounding of oxytocic drugs to obtain in the present invention, the following single factor experiment has been carried out:
[0079] Carry out broodstock cultivation and selection according to the broodstock cultivation method in the experimental group of Example 1 of the present invention, select 32 female fishes and 16 male fishes with the same specification and the same cultivation time, and be randomly divided into four groups, which are respectively experimental group 1, experimental group Group 2, experimental group 3 and control group, 8 female fish and 4 male fish in each group, artificially induce labor for experimental group 1-3: the oxytocin drug for experimental group 1 is chorionic gonadotropin 1500IU / mL + luteinizing hormone Release hormone analogue No. 2 200μg / mL + dioconone maleate 10mg / mL mixture; the oxytocin drug selected for experimental group 2 was chorionic gonadotropin 1500...
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