Culture solution for promoting in-vitro maturation of porcine oocytes

An oocyte, in vitro mature technology, applied in the direction of cell culture active agent, germ cell, culture process, etc., can solve the problems of weak developmental potential, small number of cells in the inner cell mass, and inability to accurately restore the maternal microenvironment. Effects of improving embryo development rate and quality, and promoting maturation rate and quality

Active Publication Date: 2020-04-03
HUAZHONG AGRI UNIV
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  • Description
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Problems solved by technology

However, embryos obtained by the above techniques have many developmental problems, including low rate of blastocyst development, low number of cells in the inner cell mass, low birth efficiency of animals, etc.
One of the main reasons for these proble

Method used

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  • Culture solution for promoting in-vitro maturation of porcine oocytes
  • Culture solution for promoting in-vitro maturation of porcine oocytes
  • Culture solution for promoting in-vitro maturation of porcine oocytes

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Embodiment Construction

[0017] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0018] Through the analysis of transcriptome data, we found that porcine ovary and oviduct tissues have high levels of WNT5A expression, while oocytes also express the receptor protein FZD5 of WNT5A, thus speculating that WNT5A may be involved in porcine oocyte maturation play an important role. Therefore, in order to make the components of the porcine oocyte maturation medium in vitro tend towards the composition of the maternal microenvironment, the present invention achieves the purpose of promoting the in vitro maturation rate and quality of porcine oocytes by artificially adding factors such as WNT5A.

[0019] (1) Reagent source and preparation

[0020] M199 basal culture medium without HEPES is the product of Thermo Fisher Scientific, WNT5A (mouse source) is the product of Merck Millipore, and other unspecified products are the products...

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Abstract

The invention discloses a culture solution for promoting in-vitro maturation of porcine oocyte, the culture solution takes an M199 culture solution without HEPES as a basic culture solution, and porcine follicular fluid, L-cysteine, sodium pyruvate, epidermal growth factor, insulin, gonadotropin, chorionic gonadotropin and WNT5A cytokine are also added. When the culture solution is used for carrying out in-vitro culture on the porcine oocytes, the in-vitro maturation efficiency and quality of the oocytes can be effectively improved, the obtained mature oocytes are used for carrying out porcinein-vitro fertilization and somatic cell nuclear transfer embryo production, and the blastocyst development rate and quality of the mature oocytes are also remarkably improved.

Description

technical field [0001] The invention belongs to the field of livestock embryo engineering, in particular to a culture solution for promoting in vitro maturation of pig oocytes. Background technique [0002] In vitro fertilization and somatic cell nuclear transfer are the two most common technologies in the field of livestock embryo engineering, which can be applied to multiplication of fine-bred livestock, construction of animal models, and production of transgenic animals. However, the embryos obtained by the above techniques have many developmental problems, including low blastocyst development rate, low number of inner cell mass cells, and low birth efficiency of animals. One of the main reasons for these problems is that the culture system of in vitro maturation of oocytes cannot accurately restore the microenvironment provided by the mother, resulting in significantly weaker developmental potential of oocytes matured in vitro than in vivo matured oocytes. [0003] At p...

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Application Information

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IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/30C12N2500/32C12N2500/84C12N2501/11C12N2501/31C12N2501/33C12N2501/415
Inventor 苗义良刘鑫张霞周吉隆李哲坤郝宇晨
Owner HUAZHONG AGRI UNIV
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