Culture solution for promoting in-vitro maturation of porcine oocytes

Abstract

The invention discloses a culture solution for promoting in-vitro maturation of porcine oocyte, the culture solution takes an M199 culture solution without HEPES as a basic culture solution, and porcine follicular fluid, L-cysteine, sodium pyruvate, epidermal growth factor, insulin, gonadotropin, chorionic gonadotropin and WNT5A cytokine are also added. When the culture solution is used for carrying out in-vitro culture on the porcine oocytes, the in-vitro maturation efficiency and quality of the oocytes can be effectively improved, the obtained mature oocytes are used for carrying out porcinein-vitro fertilization and somatic cell nuclear transfer embryo production, and the blastocyst development rate and quality of the mature oocytes are also remarkably improved.

Description

technical field [0001] The invention belongs to the field of livestock embryo engineering, in particular to a culture solution for promoting in vitro maturation of pig oocytes. Background technique [0002] In vitro fertilization and somatic cell nuclear transfer are the two most common technologies in the field of livestock embryo engineering, which can be applied to multiplication of fine-bred livestock, construction of animal models, and production of transgenic animals. However, the embryos obtained by the above techniques have many developmental problems, including low blastocyst development rate, low number of inner cell mass cells, and low birth efficiency of animals. One of the main reasons for these problems is that the culture system of in vitro maturation of oocytes cannot accurately restore the microenvironment provided by the mother, resulting in significantly weaker developmental potential of oocytes matured in vitro than in vivo matured oocytes. [0003] At p...

AI Technical Summary

Problems solved by technology

However, embryos obtained by the above techniques have many developmental problems, including low rate of blastocyst development, low number of cells in the inner cell mass, low birth efficiency of animals, etc.

One of the main reasons for these problems is that the culture system for in vitro maturation of oocytes cannot accurately restore the microenvironment provided by the mother, resulting in significantly weaker developmental potential of in vitro matured oocytes than in vivo matured oocytes.

Images