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Method for establishing horseshoe kidney animal model

An animal model, horseshoe kidney technology, applied in the field of horseshoe kidney animal model creation, can solve the problems of low probability of horseshoe kidney deformity and poor repeatability, and achieve good repeatability and good experimental repeatability

Inactive Publication Date: 2016-11-30
GENERAL HOSPITAL OF PLA
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  • Abstract
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  • Claims
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Problems solved by technology

Among them, the occurrence of horseshoe kidney deformity is purely accidental. Repeat the experiment according to the method recorded in this document, the probability of occurrence of horseshoe kidney deformity is low, and the repeatability is poor.

Method used

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  • Method for establishing horseshoe kidney animal model
  • Method for establishing horseshoe kidney animal model
  • Method for establishing horseshoe kidney animal model

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Embodiment Construction

[0018] The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples. For the experimental methods that do not specify specific conditions in the following examples, select according to conventional methods and conditions, or according to the product instructions.

[0019] All experimental animals were approved by the Experimental Animal Ethics Committee before conducting animal experiments.

[0020] Example, Creation and Improvement of Retinoic Acid-Induced Horseshoe Kidney Mouse Model

[0021] 1) Select 8-week-old sexually mature ICR mice, 4 females and 1 male in each cage, and mate the males and females. Check the female rats every day. If the female rats are found to have vaginal plugs, they are considered pregnant. Mark the rats, record the pregnancy time, and transfer them to single cages.

[0022] 2) Divide the pregnant female mice into 3 large groups and 9 subgroups according to the chro...

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Abstract

The invention provides a method for establishing a horseshoe kidney animal model. The method comprises: injecting a certain amount of retinoic acid solution at one time in the early pregnancy of the pregnant animal, and routinely feeding and giving birth to obtain the horseshoe kidney animal model. The method for creating an animal model of horseshoe kidney of the present invention has good experimental repeatability, and provides an experimental tool and platform for mechanism research for abnormal kidney development such as horseshoe kidney. Surgical operation and drug evaluation provide an extremely close and convenient experimental tool platform and scientific basis.

Description

technical field [0001] The invention relates to a method for creating an animal model of horseshoe kidney, in particular, the invention relates to a method for constructing an animal model of horseshoe kidney by injecting retinoic acid into pregnant animals. Background technique [0002] Congenital anomalies of the kidney and urinary tract (CAKUT) include various structural malformations during embryonic kidney development. CAKUT accounts for 20%-30% of all congenital malformations, and foreign literature reports that the prevalence of CAKUT is 3‰-6‰; domestic retrospective analysis of the monitoring data of more than 1.4 million birth defects in Beijing in the past 7 years found that Beijing The proportion of congenital kidney malformations in the city was 1.25‰. European dialysis registry data show that CAKUT is the leading cause of end-stage renal disease in children, with approximately 41.3% of children receiving renal replacement therapy. More than 200,000 patients in...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 谢院生齐焰胡盼盼李清刚陈香美
Owner GENERAL HOSPITAL OF PLA
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