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Biomarker for predicting pregnancy outcome of infertility patient and application of biomarker

A biomarker, infertility technology, applied in the field of biology and reproductive medicine, can solve the problems of low cytokines, inconclusive, lack of accuracy, etc., to achieve reliable and sensitive detection basis, good prediction of pregnancy outcome, broad social significance Effect

Active Publication Date: 2020-11-20
深圳市锦欣医疗科技创新中心有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual operation process, this detection method has found many problems: First, the content of cytokines in the human body is very small, especially the anti-inflammatory cytokines secreted by Th2 cells, and the current methodology cannot accurately measure them. Secondly, the current detection methods for cytokines are still inconclusive, and the results obtained by different methods are quite different and lack accuracy; finally, the current detection of cytokines is mainly for peripheral blood samples, and it is still not accurate and accurate. Effectively detect the expression of cytokines in tissue

Method used

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  • Biomarker for predicting pregnancy outcome of infertility patient and application of biomarker
  • Biomarker for predicting pregnancy outcome of infertility patient and application of biomarker
  • Biomarker for predicting pregnancy outcome of infertility patient and application of biomarker

Examples

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Effect test

Embodiment 1

[0041] This example relates to endometrial scraping of the endometrium in the mid-luteal phase of infertile patients. Infertile patients undergo a natural cycle or an alternative cycle plan, and use a disposable endometrial sampling tube for endometrial sampling 7-9 days after ovulation. The specific method is as follows:

[0042] (1) Washing and disinfection: wash the vulva with 0.1% (percentage by weight) Anduofu, and wash the vagina twice;

[0043] (2) Putting the speculum: Put the speculum into the vagina in a closed manner, then open the speculum to expose the cervix and fix it;

[0044] (3) Sampling: Put the sampling tube into the uterine cavity from the cervix, adjust the angle according to the position of the patient's uterus, and pull the needle core of the sampling tube outward to the position of the cervix after reaching the fundus of the uterus, and pull the sampling tube back and forth to suck the tissue, 6-8mm That is, take out the sampling tube; put the taken ...

Embodiment 2

[0046] This embodiment involves pretreatment of the endometrial tissue obtained in Example 1, the specific method is as follows:

[0047] (1) Tissue cleaning: the endometrial tissue obtained in Example 1 was cleaned, and obvious blood clots and mucus on the tissue were removed in physiological saline;

[0048] (2) Tissue fixation: transfer the cleaned tissue into a glass bottle filled with formalin fixative, and fix it for 4 hours;

[0049] (3) Tissue dehydration: Wrap the isolated tissue with gauze and put it into the embedding box; put the embedding box into the dehydrator, start the program to run; fix: 4% formalin for 2h40min; gradient dehydration: 80 % ethanol 1h → 95% ethanol Ⅰ 1h → 95% ethanol Ⅱ 1h → anhydrous ethanol Ⅰ 1h → anhydrous ethanol Ⅱ 1h; transparent: xylene Ⅰ 35min → xylene Ⅱ 20min → xylene Ⅲ 20min; dipping wax: paraffin Ⅰ 1h20min → paraffin Ⅱ 1h20min → paraffin Ⅲ 1h20min; remove the sample;

[0050] (4) Tissue embedding: Take out a suitable embedding mold ...

Embodiment 3

[0053] This embodiment involves immunohistochemical detection of the expression of T-bet and GATA3 in the endometrial tissue of the dried section obtained in Example 2. The specific method is as follows:

[0054] (1) The dried slices obtained in Example 2 were dewaxed with xylene, hydrated with an alcohol gradient, and then rinsed with deionized water to obtain hydrated slices;

[0055] (2) Incubate with 30% hydrogen peroxide, wash with water, and obtain endogenous peroxidase-inactivated slices;

[0056] (3) Put the slices into the antigen retrieval solution for microwave repair, after 15 minutes, place them at room temperature to cool, wash with water, and obtain slices after antigen retrieval;

[0057] (4) Shake off the excess liquid on the slice, add non-specific protein blocking agent and incubate at room temperature for 20 minutes to obtain the blocked slice;

[0058] (5) Shake off the non-specific protein blocking agent, add T-bet / GATA3 antibody respectively, incubate i...

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Abstract

The invention discloses a biomarker for predicting pregnancy outcome of an infertility patient and application of the biomarker, and belongs to the technical field of biology and reproductive medicine. The biomarker for predicting the pregnancy outcome of the infertility patient is a quantity ratio of T-bet molecules to GATA3 molecules. Experiments show that the quantity ratio of the T-bet molecules to the GATA3 molecules can be used as a biomarker for predicting pregnancy outcome of infertility patients, and the biomarker can be used as a prediction and prevention target for early pregnancy failure of the infertility patients, and has positive pharmaceutical value and wide social significance. In addition, the invention also discloses a kit of the biomarker for predicting the pregnancy outcome of the infertility patient.

Description

technical field [0001] The invention relates to a biomarker for predicting the pregnancy outcome of infertility patients and its application, belonging to the technical field of biology and reproductive medicine. Background technique [0002] With the change of the ecological environment and the increase of the age of marriage and childbearing, the prevalence of infertility in my country is on the rise, and it has become a major reproductive disease that seriously affects the health of my country's population and social development. In Vitro Fertilization-Embryo Transfer (IVF-ET) is the most effective method for treating infertility. Since its inception, it has brought hope to countless infertile families to have babies. However, there are still many women who still have transplantation failure or early miscarriage after IVF-ET. Therefore, there is an urgent need for some indicators to evaluate and predict the pregnancy outcome of infertile patients in order to prevent pregn...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/689G01N2333/47
Inventor 李玉叶余姝毅黄春宇连若纯刁梁辉杜晨阳陈聪黎心缘曾勇
Owner 深圳市锦欣医疗科技创新中心有限公司
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