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A biomarker for predicting pregnancy outcome in infertile patients and its application

A technology for infertility and patients, which is applied in the field of biology and reproductive medicine, can solve the problems of small cytokines, inaccurate detection, and inconclusiveness, and achieve sensitive detection basis, reliable detection basis, and good prediction effect

Active Publication Date: 2022-07-12
深圳市锦欣医疗科技创新中心有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual operation process, this detection method has found many problems: First, the content of cytokines in the human body is very small, especially the anti-inflammatory cytokines secreted by Th2 cells, and the current methodology cannot accurately measure them. Secondly, the current detection methods for cytokines are still inconclusive, and the results obtained by different methods are quite different and lack accuracy; finally, the current detection of cytokines is mainly for peripheral blood samples, and it is still not accurate and accurate. Effectively detect the expression of cytokines in tissue

Method used

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  • A biomarker for predicting pregnancy outcome in infertile patients and its application
  • A biomarker for predicting pregnancy outcome in infertile patients and its application
  • A biomarker for predicting pregnancy outcome in infertile patients and its application

Examples

Experimental program
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Embodiment 1

[0041] This example involves endometrial scratching of the midluteal endometrium of an infertile patient. For infertile patients, natural cycle or alternative cycle plan, 7-9 days after ovulation, use a disposable endometrial sampling tube for endometrial sampling. The specific method is as follows:

[0042] (1) Rinse and disinfect: rinse the vulva and the vagina twice with 0.1% (weight percent) Andover;

[0043] (2) Putting the speculum: put the speculum into the vagina in a closed manner, then open the speculum, expose the cervix, and fix it;

[0044] (3) Sampling: put the sampling tube into the uterine cavity from the cervix, adjust the angle according to the position of the patient's uterus, pull the core of the sampling tube outward to the position of the cervix after reaching the uterine fundus, and pull the sampling tube back and forth to suction the tissue, 6-8mm That is, take out the sampling tube; put the taken out endometrial tissue into a petri dish already fille...

Embodiment 2

[0046] The present embodiment relates to the pretreatment of the endometrial tissue obtained in Example 1, and the specific method is as follows:

[0047] (1) tissue cleaning: the endometrial tissue obtained in Example 1 is cleaned, and the obvious blood clots and mucus on the tissue are removed in physiological saline;

[0048] (2) Tissue fixation: transfer the cleaned tissue into a glass bottle containing formalin fixative, and fix for 4 hours;

[0049] (3) Tissue dehydration: wrap the isolated tissue with gauze and place it in the embedding box; put the embedding box into the dehydrator, and start the program to run; fixation: 4% formalin for 2h40min; gradient dehydration: 80 % ethanol 1h→95% ethanol Ⅰ 1h→95% ethanol Ⅱ 1h→absolute ethanol Ⅰ 1h→absolute ethanol Ⅱ 1h; transparent: xylene Ⅰ 35min→xylene Ⅱ 20min→xylene Ⅲ 20min; immersion wax: paraffin Ⅰ 1h20min→paraffin Ⅱ 1h20min→paraffin Ⅲ 1h20min; remove the sample;

[0050] (4) Tissue embedding: take out a suitable embeddi...

Embodiment 3

[0053] The present embodiment relates to immunohistochemical detection of the expression of T-bet and GATA3 in endometrial tissue on the dried sections obtained in Example 2, and the specific methods are as follows:

[0054] (1) the dried section obtained in Example 2 is dewaxed with xylene, hydrated with an alcohol gradient, and rinsed with deionized water to obtain the hydrated section;

[0055] (2) incubating with 30% hydrogen peroxide and washing with water to obtain sections after inactivation of endogenous peroxidase;

[0056] (3) Put the slices into the antigen retrieval solution for microwave repair, place them to cool at room temperature after 15 minutes, and wash with water to obtain the slices after antigen retrieval;

[0057] (4) Throwing off the excess liquid on the slice, adding a non-specific protein blocking agent and incubating at room temperature for 20 min to obtain the blocked slice;

[0058] (5) Throw off the non-specific protein blocking agent, add T-bet...

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Abstract

The invention discloses a biological marker for predicting the pregnancy outcome of an infertile patient and an application thereof, belonging to the technical field of biology and reproductive medicine. The biomarker for predicting pregnancy outcome in infertile patients is the quantitative ratio of T-bet molecule and GATA3 molecule. The applicant has found through experiments that the quantitative ratio of T-bet molecules and GATA3 molecules can be used as a biomarker for predicting the pregnancy outcome of infertile patients, and this biomarker can be used as a prediction and prevention target for early pregnancy failure in infertile patients point, has positive pharmaceutical value and broad social significance. In addition, the present invention also discloses the above-mentioned kit of biomarkers for predicting the pregnancy outcome of infertile patients.

Description

technical field [0001] The invention relates to a biomarker for predicting the pregnancy outcome of an infertile patient and its application, belonging to the technical field of biology and reproductive medicine. Background technique [0002] With the change of the ecological environment and the increasing age of marriage and childbearing year by year, the prevalence of infertility in my country has shown a rising trend, and it has become a major reproductive disease that seriously affects the health and social development of my country's population. In Vitro Fertilization-Embryo Transfer (IVF-ET), as the most effective method to treat infertility, has brought hope to countless infertile families to have babies since its inception. However, there are still many women who still have transplant failure or early miscarriage after IVF-ET. Therefore, some clinical indicators are urgently needed to evaluate and predict the pregnancy outcome of infertile patients to prevent pregnan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/689G01N2333/47
Inventor 李玉叶余姝毅黄春宇连若纯刁梁辉杜晨阳陈聪黎心缘曾勇
Owner 深圳市锦欣医疗科技创新中心有限公司
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