CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof

A sensing method and biosensor technology, applied in the field of gold nanometer SPR sensor and its preparation, can solve problems such as false positives, and achieve the effects of convenient operation, low detection cost, and increased SPR angle
CN110646382AActive Publication Date: 2020-01-03SHANGHAI UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI UNIV
Publication Date
2020-01-03

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Abstract

The invention discloses an SPR biosensing system for detecting a CTC. The SPR biosensing system includes an SPR biosensor, a cell membrane functionalized nanoparticle and a folate modified nanoparticle; the SPR biosensor includes an immunochip having a surface modified by a JUP antibody; the cell membrane functionalized nanoparticle contains a JUP protein and a folate receptor; the cell membrane functionalized nanoparticle is combined with the immunochip via an interaction of an antigen-antibody; and the folate modified nanoparticle is combined with the cell membrane functionalized nanoparticle via an interaction of a folate-folate receptor. According to the SPR biosensing system provided by the invention, the CTC is combined at dual selectivity by using the cell membrane functionalized nanoparticle and the folate modified nanoparticle, a false positive problem caused by single antigen-antibody specificity identification is improved, the detection cost is low, and the operation is convenient.
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Description

technical field

[0001] The invention relates to the field of detection of circulating tumor cells, in particular to an SPR sensor based on cell membrane functionalized gold nanometers and a preparation method thereof. Background technique

[0002] Circulating tumor cells (CTCs), a class of cells released from neoplastic lesions into the blood system, play a key role in the spread of metastatic cancer. CTCs can reflect the risk of tumor metastatic progression and can facilitate precision therapy by detecting CTCs in blood. Therefore, the detection of CTCs is very important for cancer. Currently, CTCs are mainly enriched and detected based on their biological or physical properties, such as specific proteins on the cell surface, cell size, and charged properties of the cells. Commonly used CTC detection methods include immunomagnetic bead method and microfluidic technology. In addition, CellSearch, invented by Veridex, is the only commercial product approved by FDA and CFDA...

Claims

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