Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof

A sensing method and biosensor technology, applied in the field of gold nanometer SPR sensor and its preparation, can solve problems such as false positives, and achieve the effects of convenient operation, low detection cost, and increased SPR angle

Active Publication Date: 2020-01-03
SHANGHAI UNIV
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is how to improve the sensitivity and specificity of detecting CTC, and how to improve the false positive problem of specific recognition by a single antigen antibody in the process of detecting CTC

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof
  • CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof
  • CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The following describes several preferred embodiments of the present invention with reference to the accompanying drawings, so as to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned herein.

[0035] In the present invention, the gold nanoparticles wrapped in the cell membrane can be replaced with other nanoparticles, including magnetic nanoparticles and nanometer quantum dots, so as to achieve different detection purposes.

[0036] The principle of the present invention is to simulate artificial CTC samples by overexpressing JUP and adding human breast cancer cell line MCF-7. Prolinker is a calixarene derivative containing -SH. Prolinker can bind to the chip surface through gold-sulfhydryl bonds. The calixarene cavity of Prolinker can be used to anchor JUP antibodies to the chip surface. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an SPR biosensing system for detecting a CTC. The SPR biosensing system includes an SPR biosensor, a cell membrane functionalized nanoparticle and a folate modified nanoparticle; the SPR biosensor includes an immunochip having a surface modified by a JUP antibody; the cell membrane functionalized nanoparticle contains a JUP protein and a folate receptor; the cell membrane functionalized nanoparticle is combined with the immunochip via an interaction of an antigen-antibody; and the folate modified nanoparticle is combined with the cell membrane functionalized nanoparticle via an interaction of a folate-folate receptor. According to the SPR biosensing system provided by the invention, the CTC is combined at dual selectivity by using the cell membrane functionalized nanoparticle and the folate modified nanoparticle, a false positive problem caused by single antigen-antibody specificity identification is improved, the detection cost is low, and the operation is convenient.

Description

technical field [0001] The invention relates to the field of detection of circulating tumor cells, in particular to an SPR sensor based on cell membrane functionalized gold nanometers and a preparation method thereof. Background technique [0002] Circulating tumor cells (CTCs), a class of cells released from neoplastic lesions into the blood system, play a key role in the spread of metastatic cancer. CTCs can reflect the risk of tumor metastatic progression and can facilitate precision therapy by detecting CTCs in blood. Therefore, the detection of CTCs is very important for cancer. Currently, CTCs are mainly enriched and detected based on their biological or physical properties, such as specific proteins on the cell surface, cell size, and charged properties of the cells. Commonly used CTC detection methods include immunomagnetic bead method and microfluidic technology. In addition, CellSearch, invented by Veridex, is the only commercial product approved by FDA and CFDA...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 陈红霞
Owner SHANGHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com