Chip for SPR bioreactor as well as preparation method and application of chip

A biosensor and chip technology, applied in the field of optical sensors, can solve the problems of complex preparation process and non-universal application range

Inactive Publication Date: 2015-04-29
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This technology uses graphene nano-gold to label ractopamine molecularly imprinted polymers to enhance the SPR sensing signal, but its pre...

Method used

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  • Chip for SPR bioreactor as well as preparation method and application of chip
  • Chip for SPR bioreactor as well as preparation method and application of chip

Examples

Experimental program
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Effect test

Embodiment 1

[0033] This embodiment includes the following steps:

[0034] 1) Preparation of graphene / polymer composite: Dissolve 10 mg of graphene with a sheet diameter of less than 200 nm in 10 mL of N, N-dimethylformamide solution for 30 min and ultrasonically disperse it with 1 g of polyethylmethacrylate Esters (Mw=100000) in 20mL N, N-dimethylformamide were mixed evenly.

[0035] 2) Take a certain amount of the prepared graphene / polymer composite and drop it on the glass substrate used to prepare the SPR sensor chip, and spin coat it through a coater at a speed of 6000r / min for 10s. Afterwards, put the glass slide in a vacuum oven and dry it at 100°C for 6 hours to completely evaporate the solvent.

[0036] 3) The chip can be obtained by evaporating a layer of gold film with a thickness of 40 nm through a magnetron sputtering evaporator.

[0037] Graphene in the above operation can also be replaced by graphene oxide.

Embodiment 2

[0039] This embodiment includes the following steps:

[0040] 1) Preparation of graphene oxide / polymer composite:

[0041]1.1) 50mg of graphene oxide with sheet diameter less than 100nm, 76mg of 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride, 125mg of N‐hydroxysuccinimide, 21mg2,3 ‐Dibromopropionamide was mixed in 20 mL of aqueous solution for 15 minutes to covalently link 2,3‐dibromopropionamide to graphene oxide.

[0042] 1.2) Take 50mL of a 1:1 mixture of water and methanol into a 250mL single-necked round bottom flask, add 5g of methyl methacrylate monomer, 50mg of CuCl 2 , 120mg 2,2-bipyridyl, 70mg ascorbic acid, mix well.

[0043] 1.3) Add the graphene oxide mixed solution to the solution system, stir at room temperature and react under nitrogen protection for 24 hours. After the gray-white viscous substance appears in the system, filter it. After cleaning the white viscous substance with deionized water several times, vacuum oven Dry at 60°C.

[0044] 2...

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Abstract

The invention provides a chip for an SPR bioreactor in the field of optical sensors as well as a preparation method and application of the chip. The chip is composed of a glass substrate, a graphene or graphene oxide/polymer compound film and a gold film generated in an evaporation manner in order from bottom to top, wherein the polymer compound is polyacrylic acid or a polyacrylate. According to the preparation method, a layer of graphene/polymer compound film material is added between the glass substrate and the gold film, and the detection sensitivity of the SPR sensor is enhanced by virtue of the surface plasma resonance effect, which is better than that of the gold film, of the graphene; the SPR sensor manufactured by use of the method is high in sensitivity, simple and convenient in preparation method, and widely applicable to the fields such as biology, medicine, chemistry and environments.

Description

technical field [0001] The present invention relates to a technology in the field of optical sensors, in particular to a SPR (Surface Plasmon Resonance, Surface Plasmon Resonance) biosensor chip and its preparation method and application. Background technique [0002] Biosensor is an important field of bioanalysis technology. It is an emerging technology that has grown up through the interpenetration of biology, chemistry, medicine, physics, electronic technology and other disciplines. It has created a strong vitality and provided powerful help for human beings to deepen their understanding of the essential phenomenon of life and the interaction between life and the environment. Among them, the surface plasmon resonance (SPR) optical sensor has a wide range of applications in life sciences, drug development, medical diagnosis, agriculture, food, public safety, environmental pollution and other fields due to its high sensitivity, label-free, and real-time detection. Prospect...

Claims

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Application Information

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IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 郭守武王艳梅吴海霞
Owner SHANGHAI JIAO TONG UNIV
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