Highly-sensitive infrared band SPR biosensor

Abstract

The invention provides a highly-sensitive infrared band SPR biosensor, and belongs to the technical field of biologic sensing. A halogen tungsten lamp provides a broadband light source, a multi-mode optical fiber is adopted to transmit lights, the lights are transmitted to a prism coated with a metal film by using a collimating lens in order to generate an SPR effect, an output optical signal is input to a computer through a spectrometer, and a compiled software program is used to process, analyze and store experiment data. a method disclosed in the invention greatly increases the sensitivity of a sensing system, and the sensitivity to an SPR signal with the wavelength in an infrared band is one order of magnitude higher than the sensitivity to an SPR signal with the wavelength in a visible band. A prism SPR sensing element adopted in the invention has good real-time responsiveness and stability, so the biosensor realizes high-sensitivity, high-resolution and high-precision detection, and can be used in real-time rapid detection of the characteristics of micro-concentration proteins, biological micro-molecules and cells.

Description

technical field [0001] The invention belongs to the technical field of biosensing, uses a surface plasmon resonance (SPR) sensor element based on a prism structure to tune a detection signal to an infrared band, and relates to the principle of the surface plasmon resonance (SPR). The whole system is simple in structure and easy to operate, can realize high-sensitivity and high-resolution measurement of biological samples, and has broad application prospects in the field of biological small molecule sensing. Background technique [0002] Surface Plasmon Resonance (SPR) sensing technology is a new type of photoelectric detection technology. When light is incident on the surface of a prism coated with a certain thickness of metal film, total reflection will occur. When certain conditions are met, the evanescent wave formed will resonate with the plasma wave in the medium, so the incident light intensity will be greatly attenuated, that is For the SPR effect. Because it has go...

AI Technical Summary

Problems solved by technology

At present, due to the limitation of sensitivity and resolution, SPR sensors have certain limitations in the fields of protein small molecule binding and cell characteristic detection. Therefore, realizing high-sensitivity detection is the development trend of SPR detection technology at present.

At present, most SPR sensors are mostly used in the field of macromolecular detection, and can only detect large molecular weight biological samples. Therefore, the design of high-sensitivity and high-resolution SPR sensors has become an urgent need in the field of small molecule detection.

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