Highly-sensitive infrared band SPR biosensor

A biosensor and infrared band technology, applied in the field of biosensing, achieves the effect of improving sensitivity, simple manufacturing process, high-precision real-time and fast detection

Inactive Publication Date: 2017-06-16
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the limitation of sensitivity and resolution, SPR sensors have certain limitations in the fields of protein small molecule binding and cell characteristic detection. Therefore, realizing high-sensitivity detection is the development trend of SPR detection technology at prese

Method used

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  • Highly-sensitive infrared band SPR biosensor
  • Highly-sensitive infrared band SPR biosensor
  • Highly-sensitive infrared band SPR biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] 1. Preparation process

[0018] 1) Deposit a 50nm metal thin film on one side of the prism 1 by magnetron sputtering technology, and fix it on the support;

[0019] 2) Install the flow cell 9 on the surface of the prism 1 for the transmission of the sample 3 to be tested;

[0020] 3) SMA905 is used as the connector, and the multimode optical fiber 14 guides the light of the broadband light source 10 into the collimating lens 11, and projects the light beam onto the surface of the prism 1, and the incident angle of the light source is less than 90 degrees;

[0021] 4) The light emitted from the prism 1 passes through the collimator lens 11 again, and is introduced into the spectrometer 12 through the multimode optical fiber 14;

[0022] 5) Transfer the data in the spectrometer 12 to the computer 13 by using the USB data cable 15 for data processing and analysis.

[0023] 2. Operation

[0024] 1) The test device such as figure 2 shown. Before the experiment, when th...

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PUM

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Abstract

The invention provides a highly-sensitive infrared band SPR biosensor, and belongs to the technical field of biologic sensing. A halogen tungsten lamp provides a broadband light source, a multi-mode optical fiber is adopted to transmit lights, the lights are transmitted to a prism coated with a metal film by using a collimating lens in order to generate an SPR effect, an output optical signal is input to a computer through a spectrometer, and a compiled software program is used to process, analyze and store experiment data. a method disclosed in the invention greatly increases the sensitivity of a sensing system, and the sensitivity to an SPR signal with the wavelength in an infrared band is one order of magnitude higher than the sensitivity to an SPR signal with the wavelength in a visible band. A prism SPR sensing element adopted in the invention has good real-time responsiveness and stability, so the biosensor realizes high-sensitivity, high-resolution and high-precision detection, and can be used in real-time rapid detection of the characteristics of micro-concentration proteins, biological micro-molecules and cells.

Description

technical field [0001] The invention belongs to the technical field of biosensing, uses a surface plasmon resonance (SPR) sensor element based on a prism structure to tune a detection signal to an infrared band, and relates to the principle of the surface plasmon resonance (SPR). The whole system is simple in structure and easy to operate, can realize high-sensitivity and high-resolution measurement of biological samples, and has broad application prospects in the field of biological small molecule sensing. Background technique [0002] Surface Plasmon Resonance (SPR) sensing technology is a new type of photoelectric detection technology. When light is incident on the surface of a prism coated with a certain thickness of metal film, total reflection will occur. When certain conditions are met, the evanescent wave formed will resonate with the plasma wave in the medium, so the incident light intensity will be greatly attenuated, that is For the SPR effect. Because it has go...

Claims

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Application Information

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IPC IPC(8): G01N21/552
CPCG01N21/553
Inventor 彭伟陈诗蒙刘云
Owner DALIAN UNIV OF TECH
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