Dual-channel MDOF (multidegree of freedom) adjustment mechanism of optical surface plasma resonance biosensor

Abstract

The invention relates to a dual-channel MDOF (multidegree of freedom) adjustment mechanism of an optical surface plasma resonance biosensor. The mechanism comprises a biochip assembly regulating unit, an optical path regulating unit, an angle regulating unit and a supporting and guiding unit, wherein the optical path regulating unit comprises a laser swing frame and a charge coupled device (CCD) swing frame; the angle regulating unit comprises an elevating assembly, a rotating support arm, a transmission shaft and a driving device; driven by the driving device, the transmission shaft drives the elevating assembly to complete the elevating travel, and at the same time, drives the laser swing frame and the CCD swing frame to form an included angle within a certain range via the corresponding rotating support arm, and form two corresponding optical path detection passages among laser, a CCD image sensor and the biochip assembly. The dual-channel MDOF adjustment mechanism provided by the invention is convenient for operation and accurate in positioning and has high testing precision, and solves the problems, such as debugging difficulty and hard refractivity matching caused when only the biochip with a glass substrate is replaced, thereby increasing the analytic precision of the biosensor and also substantially reducing the using and maintaining cost of the optical surface plasma resonance (SPR) sensor.

Description

technical field [0001] The invention relates to the field of optical surface plasmon resonance (Surface plasmon resonance, SPR) biosensors, in particular to an optical SPR biosensor multi-degree-of-freedom adjustment mechanism. Background technique [0002] In the past 10 years, optical SPR biosensing technology has achieved rapid development in terms of implementation methods, instrument development and application field expansion. The detection mechanism of the optical SPR biosensor is as follows: the recognition film of biomolecules is fixed on the surface of the Au film of the optical SPR sensor, and the molecular recognition film captures the measured analyte, causing the refractive index of the surface of the sensor Au film to change, so it can be detected without labeling molecules. Test directly to realize non-destructive real-time online detection. Optical SPR biosensors include optical prism type, optical waveguide type, grating type, etc. according to the body of...

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Problems solved by technology

For example, the Chinese patent document CN101539570A discloses a coupling device for an optical SPR biochip, a sensor, and a microfluidic cell, but the device can only be coupled with an integrated biosensor imported from abroad, resulting in a high overall cost of the instrument, which will inevitably increase the use of users , maintenance cost, difficult to be popularized and applied; CN102095684A discloses a multi-degree-of-freedom adjustment mechanism for an optical surface plasmon resonance biosensor, but its laser light source is fixed, and there is only one detection channel, and the adjustable angle range of incident light is limited , it is difficult to meet the detection needs of different material components

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