Method for extracting poultry feather keratin by using water insoluble ionic liquid and relative poultry feather keratin solution

The technology of poultry feathers and ionic liquid is applied to the field of poultry feather keratin solution, which can solve the problems of difficult separation of keratin, increased cost and high cost, and achieves the effects of being suitable for large-scale popularization and application, convenient for recovery and simple for separation

Inactive Publication Date: 2011-11-02
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ionic liquids are widely studied and utilized as a green solvent, but their cost is relatively high, so ionic liquids must be recycled
Although the above-mentioned hydrophilic ionic liquids have a good dissolving effect on silk, feathers, etc., the separation of keratin and the recovery of ionic liquids require the addition of organic solvents, resulting in increased costs and environmental pollution.
[0005] Taking a broad view of the above-mentioned prior art, the defects of the traditional method for extracting keratin are: the amount of reagents such as inorganic acids and bases is large, and cannot be recycled, causing great pollution to the environment; the defect of ionic liquids for extracting keratin is: it is difficult isolated from the ionic liquid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Ionic liquid [HOEMIm]NTf 2 Determination of Solubility in Water

[0032] 1) Full-wavelength scanning of ionic liquids

[0033] Accurately weigh 50mg of ionic liquid [HOEMIm]NTf 2 (Lanzhou Institute of Physics and Chemistry, Chinese Academy of Sciences, purity>99%) was placed in a volumetric flask, and deionized water was added to constant volume to prepare a 1.0 mg / mL standard solution. Using deionized water as a blank reference to carry out full-wavelength scanning in the wavelength range of 190-300nm, the maximum absorption peak of the ionic liquid can be obtained at 217.5nm.

[0034] 2) Determination of ionic liquid standard curve

[0035] Take 7 test tubes respectively, add the prepared 1.0mg / mL ionic liquid [HOEMIm]NTf 2 Solutions 0, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6mL were added to each tube with deionized water to a total volume of 5mL to obtain the solution to be tested. Using deionized water as a reference, at the maximum absorption wavelength o...

Embodiment 2～11

[0038]Embodiment 2~11 adopts ionic liquid to extract chicken feather keratin

[0039] The basic process that embodiment 2~11 adopts is as follows:

[0040] 1) Weigh 1 g of pulverized chicken feathers and place them in a round bottom flask, and add a certain amount of water-insoluble ionic liquid [HOEMIM][NTf 2 ], sodium bisulfite and 500 μL of deionized water, mechanically stirred at 70°C to 100°C for 3 to 6 hours;

[0041] 2) After the reaction is over, first add an appropriate amount of deionized water to the reaction mixture, centrifuge to form a water-insoluble ionic liquid / undissolved chicken feather / water three-phase system, and separate the water phase; then wash the container three times with deionized water to obtain the The washing liquid and the separated aqueous phase after centrifugation are filtered together to obtain a crude keratin solution; finally, the recovery rate of the water-insoluble ionic liquid (containing a small amount of undissolved chicken feather...

Embodiment 2

[0047] Example 2: Add 20 g of water-insoluble ionic liquid, 0.3 g of sodium bisulfite, add about 1.0 g of water-insoluble ionic liquid, react at 80° C. for 4 hours, add 10 mL of deionized water to separate and extract keratin. According to calculations, the yields of keratin extracted in this example are 12.65%, 12.56%, 12.60%, 12.57%, and 12.48%, respectively, and the recovery rate of the ionic liquid can reach about 95% each time.

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PUM

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Abstract

The invention provides a method for extracting a poultry feather keratin by using water insoluble ionic liquid, comprising the follow steps: 1) washing, drying and crushing a poultry feather; 2) adding a water insoluble ionic liquid with stirring for 3-6h at the temperature of 70-100 DEG C; and 3) after the reaction, adding water, carrying out centrifugation to form a three-phase system of water insoluble ionic liquid/ undissolved poultry feather/ water, so as to obtain a crude keratin solution. In a preferred step 2), a disulfide bond reducing agent is added, and a little water is added to dissolve the disulfide bond reducing agent. The invention also provides a poultry feather keratin solution extracted by the method. The method for extracting the poultry feather keratin by using the water insoluble ionic liquid, has the advantage of simple separation of keratin, is convenient recovery of ionic liquid and friendly to environmental protection, and can be widely applied.

Description

technical field [0001] The invention relates to the technical field of poultry feather keratin extraction, in particular to a method for extracting poultry feather keratin by using a water-insoluble ionic liquid and a related poultry feather keratin solution. Background technique [0002] Keratin is a biological protein with rich sources and high application value, which widely exists in the tissue structure of organisms. The main chemical component of feather is keratin, which, as a green natural fiber, has irreplaceable advantages of other materials. The annual output of poultry feathers in the world can reach hundreds of millions of tons, and the output of feather waste in China is more than 700,000 tons, and it is still increasing year by year. Using waste feathers to prepare soluble feather protein not only makes full use of protein resources, but also greatly reduces the resulting environmental pollution. China began to pay attention to the resource utilization of fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/465C07K1/14
Inventor 曹学君王云先
Owner EAST CHINA UNIV OF SCI & TECH
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