Protein combined nanometer selenium, and preparation method and applications thereof

A combination type, protein technology, applied in the field of biomedicine, can solve the problems of limiting the nutritional and medical promotion value of nano-selenium, easy aggregation, affecting the long-term storage of nano-selenium, temperature limitation and transportation, etc., to achieve good oral bioavailability, disperse Good performance and wide storage temperature range

Active Publication Date: 2018-09-04
彭咏波
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The preparation methods involved in the above-mentioned invention patents are divided into two categories, one is to use protein, polypeptide, dextran, melatonin, chitin and mixed amino acids as templates to obtain composite nano-selenium; the other is to have antioxidant activity Phycocyanin directly reduces selenium compounds to obtain composite nano-selenium; in terms of these preparation processes, they are obtained by reacting template protein molecules and reducing agents with selenium compounds at the same time, and the resulting selenium nanocomposites are used as template molecules for surface adsorption. Mainly, it is relatively easy to aggregate, which affects the long-term storage, temperature limitation and transportation of nano-selenium in solution, thus limiting the nutritional and medical promotion value of nano-selenium

Method used

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  • Protein combined nanometer selenium, and preparation method and applications thereof
  • Protein combined nanometer selenium, and preparation method and applications thereof
  • Protein combined nanometer selenium, and preparation method and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0037] The preparation of embodiment 1 protein binding type nano elemental selenium (PSe), such as figure 1 Shown:

[0038] A. Dissolve human serum albumin (HSA) with a concentration in the range of 0.01-100 mg / mL in 10 mM GSH fresh solution (GSH phosphate buffer, pH 5.0-9.0), and react at 30 ° C Stir for 100 min to obtain a protein homogeneous solution with a final protein concentration of 50 mg / mL in which the spatial structure is developed;

[0039]B. Add 15mM sodium selenite solution to the protein homogeneous solution developed by the spatial structure to obtain a final concentration of protein in the albumin solution of 30mg / mL, stir at 4°C for 12h, and obtain light red protein-binding nanometer elemental selenium crude solution;

[0040] C. Put the crude protein-bound nanometer elemental selenium solution into a dialysis bag, the molecular cut-off of the dialysis is not less than 1000, and dialyze in a low-temperature PBS solution at 0-20°C to remove excess disulfide ...

Embodiment 2

[0042] The preparation of embodiment 2 protein binding type nano elemental selenium (PSe), such as figure 1 Shown:

[0043] A. Dissolve human serum albumin with a concentration in the range of 0.01-100 mg / mL in 20 mM GSH fresh solution (GSH phosphate buffer, pH 5.0-9.0), react at 20°C for 20 min, and stir , to obtain a protein homogeneous solution with a final protein concentration of 50 mg / mL in which the spatial structure is expanded;

[0044] B. Add 20mM sodium selenite solution to the protein homogeneous solution developed by the spatial structure to obtain a final concentration of protein in the albumin solution of 20mg / mL, stir at 20°C for 1h, and obtain light red protein-binding nanometer elemental selenium crude solution;

[0045] C. Put the crude protein-bound nanometer elemental selenium solution into a dialysis bag, the molecular cut-off of the dialysis is not less than 1000, and dialyze in a low-temperature PBS solution at 0-20°C to remove excess disulfide bond r...

Embodiment 3

[0047] The preparation of embodiment 3 protein binding type nano elemental selenium (PSe), such as figure 1 Shown:

[0048] A. Dissolve human serum albumin (HSA) with a concentration ranging from 0.01 to 100 mg / mL in fresh 10 mM TCEP solution (TCEP phosphate buffer, pH 5.0-9.0), and react at 30°C Stir for 30 minutes to obtain a protein homogeneous solution with a final protein concentration of 70 mg / mL in which the spatial structure unfolds;

[0049] B. Add 25mM sodium selenite solution to the protein homogeneous solution developed by the spatial structure to obtain a final concentration of protein in the albumin solution of 50mg / mL, stir at 4°C for 6h, and obtain light red protein-binding nanometer elemental selenium crude solution;

[0050] C. Put the crude protein-bound nanometer elemental selenium solution into a dialysis bag, the molecular cut-off of the dialysis is not less than 1000, and dialyze in a low-temperature PBS solution at 0-20°C to remove excess disulfide bo...

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Abstract

The invention belongs to the technical field of biological medicine, and discloses protein combined nanometer selenium, and a preparation method and applications thereof. According to the preparationmethod, under the reducing action of a disulfide bond reducing agent, the internal space structure of a disulfide bond containing protein or a disulfide bond containing polypeptide is opened so as toobtain mercapto groups with reducing activity, and a part of the mercapto groups are used for converting selenium compounds into elemental selenium through reducing, the rest mercapto groups are usedfor forming intramolecular and intermolecular disulfide bonds, so that self assembling of protein combined nanometer selenium is obtained; the preparation method is capable of improving the dispersion performance and the stability of nanometer elemental selenium in solution greatly. It is confirmed by animal experiments that the active red elemental selenium is high in bioavailability and low intoxicity. The protein combined nanometer elemental selenium possesses the functions of protein and nanometer selenium, is small in nanometer particle size, is narrow in distribution range, can be stored in the state of liquid phase, is convenient for oral administration, injection, or spraying.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a protein-bound nano-selenium and its preparation method and application. Background technique [0002] Selenium is an essential trace element for human body. Selenium has important biological functions such as anti-oxidation, regulating immunity, and antagonizing harmful heavy metals. Selenium deficiency is related to more than 40 kinds of diseases, particularly serious ones such as AIDS, hepatitis B, liver cancer, Keshan disease, Kashin-Beck disease, and cardiovascular and cerebrovascular diseases. Appropriate selenium supplementation can effectively prevent and control related diseases. Regarding the selenium intake standard for normal people, the World Health Organization (WHO) stipulates that it is 40-100 μg / d, the Chinese Nutrition Society recommends that it is 60-200 μg / d, and the Canadian regulation is 98-224 μg / d. More than 40 countries and regions in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/107A61K33/04A61K47/42A61P3/02
CPCA61K47/42A61P3/02A61K33/04C07K1/1077
Inventor 彭咏波彭麓灵刘腾
Owner 彭咏波
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