8 results about "Protein combining" patented technology

The invention provides a direct purification method of an inclusion somatic cell culture. The direct purification method comprises the following steps: S1, collecting and crushing zymophyte; s2, purification of inclusion body re-suspension: re-suspending the thallus crushed substance in a first buffer solution, wherein the first buffer solution at least contains a cosolvent of the inclusion body so as to dissolve the inclusion body in the first buffer solution; combining the inclusion body protein dissolved in the first buffer solution by adopting a purification medium; washing the purification medium with a second buffer solution; and eluting and renaturating the inclusion body protein combined with the purification medium from the purification medium by adopting a third buffer solution to obtain the refolded inclusion body protein with activity. According to the method, the complex centrifugal separation step of extracting the inclusion body from the cell disruption substance is successfully omitted, the complex system of the cell disruption substance is directly incubated with the chromatography medium for purification, the operation time is greatly shortened, the operation efficiency is improved, and the degree of dependence on high-price equipment such as large and medium-sized centrifuges is greatly reduced.

The invention relates to the technical field of biological medicine, and particularly discloses a preparation method of a collagen and anti-propionibacterium acnes gamma protein combined preparation, and the preparation method of the collagen and anti-propionibacterium acnes gamma protein combined preparation comprises the following steps: preparing anti-propionibacterium acnes gamma protein; adding water into the anti-propionibacterium acnes gamma protein and the recombinant III-type collagen, stirring and mixing, adding glycerol and a neutralizing agent, and mixing to obtain a uniform solution; the preparation method comprises the following steps: dispersing carbomer with water, adding a preservative and a humectant, mixing, finally adding the uniform solution, and mixing to obtain the required collagen and anti-propionibacterium acnes gamma protein combined preparation. The combined preparation prepared by the method can be used for effectively treating acnes, is remarkable in curative effect, does not generate drug resistance, is short in treatment cycle, can quickly eliminate skin damage, promote cell growth and adhesion and repair skin barriers, and does not generate new rash after healing.

The application discloses a method for improving the determination accuracy of the content of malondialdehyde in a biological sample, which comprises adding a buffer solution, a protease solution and a thiobarbituric acid (TBA) into a malondialdehyde standard solution, and determining the absorbance of the malondialdehyde standard solution; subsequently, a standard curve is constructed, and the actual concentration of the malondialdehyde in the biological sample is calculated through the measured absorbance of the biological sample solution. The sample processing process of the method is simple, the Buffer A buffer solution and the protease used can release the MDA combined with the protein, can not only avoid the adsorption interference of the protein precipitation on the red complex of the MDA and the TBA and the extraction limitation of the supernatant, but also can detect the protein combined state MDA, can significantly improve the detection limit of the sample, is good in selectivity, improves the accuracy of the detection result, and can be used for the trace detection of clinical precious samples.

The application discloses a protein combined with NY-ESO-1 antigen, and a preparation method thereof. The preparation method comprises the following steps: performing E. coli host codon optimization on an amino acid sequence of a precursor protein combined with NY-ESO-1 antigen, constructing a nucleotide sequence of the precursor protein on a pET21b plasmid to obtain plasmid 1; transforming the plasmid 1 into E. coli; inducing culture; purifying, collecting the protein, adding thrombin, dialyzing overnight, and cutting enzymes; and further purifying to obtain the protein combined with NY-ESO-1 antigen. Experiments prove that the protein combined with NY-ESO-1 antigen can recognize NY-ESO-1 antigen K D = 6.65 μM.

The invention relates to the field of molecular biology and biological medicine, and particularly discloses a synergistic effect of combined application of Metrnl protein and a clinical first-line therapeutic drug clopidogrel for treating thrombus, and the Metrnl protein has important significance in the field of thrombotic disease treatment. According to the invention, mice are divided into four groups: a solvent control group, a Metrnl proteome, a clopidogrel group and a drug combination group. A FeCl3 induced thrombus experiment shows that thrombus formation can be inhibited by independently using Metrnl protein and clopidogrel, the thrombus formation inhibition effect is stronger by combined medication, and a synergistic effect is achieved. In addition, in the aspect of untoward effects of bleeding, compared with a clopidogrel single drug group, the risk of bleeding of the drug combination group is not obviously increased. The metrnl protein and clopidogrel are combined to enhance the antithrombotic curative effect without increasing the adverse reaction of bleeding, so that the metrnl protein and clopidogrel have good treatment potential and safety.

The invention provides a multivalent protein kinetic property prediction method and a related product. According to a specific implementation mode of the method for predicting the kinetic properties of the multivalent protein, in the process of simulating the concentration change rate of a microstate where the multivalent protein is combined with an antigen on the surface of a cell membrane, the effective concentration of the combined antigen is adopted instead of the objective concentration of the combined antigen; and thus, the accuracy of predicting the kinetic properties of the multivalent protein combined to the surface of the cell membrane can be improved.

The invention relates to a black bean protein combined modification method and application thereof, and belongs to the technical field of vegetable protein processing. The invention provides a Lactiplantibacillus plantarum ZHA-3 strain and a preparation method of the Lactiplantibacillus plantarum ZHA-3 strain. The solubility, the oil holding capacity, the foamability, the foam stability and other processing characteristics of the prepared modified black bean protein are remarkably improved, the activity loss of the plant lactobacillus after freeze drying is reduced, and the survival rate of the plant lactobacillus in the process of simulating in-vitro digestion is improved.

The invention relates to the technical field of microfluidics and in-vitro diagnosis, in particular to an integrated micro-fluidic chip for joint detection of nucleic acid and protein and a detection method, the integrated micro-fluidic chip comprises a chip top layer, a functional structure layer and a substrate; the functional structure layer is provided with a biomarker enrichment and separation area, a nucleic acid detection area, a protein detection area and a visual reading area. Wherein the enrichment and separation region adopts a configuration that the flowing direction is vertical to the direction of an electric field, and dynamic electric enrichment and synchronous separation of nucleic acid and protein are realized through an ion concentration polarization effect; an amplification or immune reaction reagent is preset in the detection area; lFA test paper is arranged in the reading area, and liquid is pressed and driven to flow through an elastic film covering the observation window. The chip can realize the whole process integration of one-step sample adding, rapid enrichment, isothermal amplification and visual reading, obviously improves the detection sensitivity and multi-index joint detection efficiency of low-abundance targets, and is suitable for the fields of infectious disease diagnosis, cancer screening, on-site rapid detection and the like.