3 results about "Protein combining" patented technology

The invention relates to the technical field of biological medicine, and particularly discloses a preparation method of a collagen and anti-propionibacterium acnes gamma protein combined preparation, and the preparation method of the collagen and anti-propionibacterium acnes gamma protein combined preparation comprises the following steps: preparing anti-propionibacterium acnes gamma protein; adding water into the anti-propionibacterium acnes gamma protein and the recombinant III-type collagen, stirring and mixing, adding glycerol and a neutralizing agent, and mixing to obtain a uniform solution; the preparation method comprises the following steps: dispersing carbomer with water, adding a preservative and a humectant, mixing, finally adding the uniform solution, and mixing to obtain the required collagen and anti-propionibacterium acnes gamma protein combined preparation. The combined preparation prepared by the method can be used for effectively treating acnes, is remarkable in curative effect, does not generate drug resistance, is short in treatment cycle, can quickly eliminate skin damage, promote cell growth and adhesion and repair skin barriers, and does not generate new rash after healing.

The application discloses a method for improving the determination accuracy of the content of malondialdehyde in a biological sample, which comprises adding a buffer solution, a protease solution and a thiobarbituric acid (TBA) into a malondialdehyde standard solution, and determining the absorbance of the malondialdehyde standard solution; subsequently, a standard curve is constructed, and the actual concentration of the malondialdehyde in the biological sample is calculated through the measured absorbance of the biological sample solution. The sample processing process of the method is simple, the Buffer A buffer solution and the protease used can release the MDA combined with the protein, can not only avoid the adsorption interference of the protein precipitation on the red complex of the MDA and the TBA and the extraction limitation of the supernatant, but also can detect the protein combined state MDA, can significantly improve the detection limit of the sample, is good in selectivity, improves the accuracy of the detection result, and can be used for the trace detection of clinical precious samples.

The invention relates to the technical field of microfluidics and in-vitro diagnosis, in particular to an integrated micro-fluidic chip for joint detection of nucleic acid and protein and a detection method, the integrated micro-fluidic chip comprises a chip top layer, a functional structure layer and a substrate; the functional structure layer is provided with a biomarker enrichment and separation area, a nucleic acid detection area, a protein detection area and a visual reading area. Wherein the enrichment and separation region adopts a configuration that the flowing direction is vertical to the direction of an electric field, and dynamic electric enrichment and synchronous separation of nucleic acid and protein are realized through an ion concentration polarization effect; an amplification or immune reaction reagent is preset in the detection area; lFA test paper is arranged in the reading area, and liquid is pressed and driven to flow through an elastic film covering the observation window. The chip can realize the whole process integration of one-step sample adding, rapid enrichment, isothermal amplification and visual reading, obviously improves the detection sensitivity and multi-index joint detection efficiency of low-abundance targets, and is suitable for the fields of infectious disease diagnosis, cancer screening, on-site rapid detection and the like.