Method for preparing lysozyme composite food packaging film

A technology of compound food and lysozyme, applied in coating and other directions, can solve the problems that new functional materials cannot be used alone, high cost, and achieve the effects of being suitable for popularization, short preparation period and simple preparation method

Active Publication Date: 2018-01-12
XIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the strict requirements of human beings on food quality and fresh-keeping effect, a single plastic film can no longer meet the requirements of new packaging, and most o...

Method used

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  • Method for preparing lysozyme composite food packaging film
  • Method for preparing lysozyme composite food packaging film

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preparation example Construction

[0041] A kind of preparation method of lysozyme composite food packaging film of the present invention, specifically implement according to the following steps:

[0042] Step 1. Prepare a neutral buffer solution of lysozyme and a disulfide bond reducing agent tris (2-carboxyethyl) phosphine hydrochloride (TCEP) solution respectively, and mix the two evenly , to prepare a compound solution, specifically implement according to the following methods:

[0043] Step 1.1. Weigh tris-hydroxymethylaminomethane hydrochloride and deionized water respectively according to the mass ratio of 1:2.5-3, then add tris-hydroxymethylaminomethane hydrochloride to the deionized water, mix and stir, wait Tris hydrochloride aqueous solution is obtained after dissolving, tris hydrochloride aqueous solution is equally divided into two parts;

[0044] Step 1.2, weigh lysozyme, the mass ratio of lysozyme to tris hydrochloride weighed in step 1.1 is 0.42~0.55:1, add the weighed lysozyme to a part of tri...

Embodiment 1

[0059] Step 1. Prepare a neutral buffer solution of lysozyme and a disulfide bond reducing agent tris (2-carboxyethyl) phosphine hydrochloride (TCEP) solution respectively, and mix the two evenly , to prepare a compound solution, specifically implement according to the following methods:

[0060] Step 1.1. Weigh trishydrochloride and deionized water respectively according to the mass ratio of 1:2.8, then add tris hydrochloride to the deionized water, mix and stir, and dissolve To obtain tris hydrochloride aqueous solution, the tris hydrochloride aqueous solution is equally divided into two parts;

[0061] Step 1.2, weigh lysozyme, the mass ratio of lysozyme to tris hydrochloride weighed in step 1.1 is 0.47:1, add the weighed lysozyme to a part of tris hydrochloride In aqueous hydrochloride solution, stir to dissolve lysozyme completely, and mark it as a neutral buffer solution of lysozyme;

[0062] Weigh three (2-carboxyethyl) phosphorus hydrochloride, the mass ratio of tris...

Embodiment 2

[0069] Step 1. Prepare a neutral buffer solution of lysozyme and a disulfide bond reducing agent tris (2-carboxyethyl) phosphine hydrochloride (TCEP) solution respectively, and mix the two evenly , to prepare a compound solution, specifically implement according to the following methods:

[0070] Step 1.1. Weigh trishydrochloride and deionized water respectively according to the mass ratio of 1:2.9, then add tris hydrochloride to the deionized water, mix and stir, and dissolve To obtain tris hydrochloride aqueous solution, the tris hydrochloride aqueous solution is equally divided into two parts;

[0071] Step 1.2, weigh lysozyme, the mass ratio of lysozyme to tris hydrochloride weighed in step 1.1 is 0.55:1, add the weighed lysozyme to a part of tris hydrochloride In aqueous hydrochloride solution, stir to dissolve lysozyme completely, and mark it as a neutral buffer solution of lysozyme;

[0072] Weigh three (2-carboxyethyl) phosphorus hydrochloride, the mass ratio of tris...

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Abstract

The invention discloses a method for preparing a lysozyme composite food packaging film. The method comprises the following steps: 1, respectively preparing a neutral buffer solution of lysozyme and adisulfide bond reducing agent tris(2-carboxyethyl)phosphorus hydrochloride solution, mixing the two solutions to be uniform so as to prepare a composite solution; 2, taking out one part of the composite solution in the step 1 and adding into a packaging bag, enabling the inner wall of the packaging bag to be completely infiltrated by the composite solution, standing, and pouring the composite solution in the packaging bag; 3, drying the packaging bag treated by the step 3, and coating a protein film layer on the inner wall of the packaging bag; 4, repeating the step 2 and step 3, superposingthe protein films layer by layer, and forming the lysozyme composite food packaging film on the surface of the packaging bag. According to the preparation method disclosed by the invention, the surface medium of an ordinary packaging material is changed, the packaging material is attached to the surface of a general package by virtue of adhesion of the proteins, the dense film can be rapidly formed, and the application effect of the packaging film in food preservation is improved.

Description

technical field [0001] The invention belongs to the technical field of preparing composite films for food packaging, and in particular relates to a preparation method of a lysozyme composite food packaging film. Background technique [0002] According to different sources, lysozyme can be divided into animal source lysozyme, plant source lysozyme and microbial source lysozyme. According to the different microorganisms it acts on, it can be divided into two categories, one is bacterial cell wall lysozyme, and the other is fungal cell wall lysozyme. The former can be divided into cell wall lysozymes acting on glycoside chains and cell wall lysozymes acting on peptide and amide moieties, and the latter can be divided into yeast cell wall lysozymes and mold cell wall lysozymes. [0003] The chemical name of lysozyme is N-acetyl murein hydrolase, its molecule is composed of 129 amino acids, 2200 atoms, and its molecular weight is 14388-18000. The basic amino acid, amide residue...

Claims

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Application Information

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IPC IPC(8): C08J7/04
Inventor 方长青张自森杨鹏蒲梦圆周星王迎曦
Owner XIAN UNIV OF TECH
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