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Method for extracting protein from [beta]-carotene fermentation liquid

A carotene and protein technology, applied in the field of protein extraction, can solve problems such as pollution, and achieve the effects of solving pollution, easy absorption and small molecular weight

Inactive Publication Date: 2018-02-09
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technology of the invention not only reduces the waste of resources, but also solves the problem of environmental pollution caused by fermentation liquid, and has a good application prospect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) carry out ultrafiltration treatment with β-carotene fermented liquid with ultrafiltration membrane, intercept the macromolecular substances such as mycelium and protein therein from waste liquid, collect interception component; The molecular weight cut-off of described ultrafiltration membrane 2000 Daltons, water inlet pressure 1.1Mpa, ultrafiltration temperature 24°C, fermentation broth pH 7.2;

[0020] (2) Add water to the retained components obtained by ultrafiltration in step (1) and stir evenly, then use ultra-low temperature quick-freezing technology to quickly freeze the retained component solution to obtain a suspension, and then use a high-pressure homogenizer to crush the obtained suspension The volume ratio of the added volume of water to the retained components is 1:3.5; the ultra-low temperature quick freezing temperature is -60°C, and the quick freezing time is 12 minutes; the pressure of the suspension passing through the high pressure homogenizer is 1...

Embodiment 2

[0027] (1) carry out ultrafiltration treatment with β-carotene fermented liquid with ultrafiltration membrane, intercept the macromolecular substances such as mycelium and protein therein from waste liquid, collect interception component; The molecular weight cut-off of described ultrafiltration membrane 2000 Daltons, water inlet pressure 1.2Mpa, ultrafiltration temperature 24°C, fermentation broth pH 6.5;

[0028] (2) Add water to the retained components obtained by ultrafiltration in step (1) and stir evenly, then use ultra-low temperature quick-freezing technology to quickly freeze the retained component solution to obtain a suspension, and then use a high-pressure homogenizer to crush the obtained suspension The volume ratio of the added volume of water to the retained components is 1:6; the ultra-low temperature quick freezing temperature is -50°C, and the quick freezing time is 12 minutes; the pressure of the suspension passing through the high pressure homogenizer is 170...

Embodiment 3

[0035] The quality detection of the recovered protein in embodiment 1 or 2 detects the mass distribution of the protein in the recovered product, and the results are shown in Table 1:

[0036] Table 1 Molecular weight distribution of recovered protein

[0037] Molecular Weight (Daltons)

[0038] As can be seen from the results of the above-mentioned quality inspection, in the protein obtained by using the extraction method of the present invention, the content of protein with a molecular weight of 1000-2000 Daltons is greater than 80%. After the protein is used as feed, It is easily absorbed by animals and has good effects.

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Abstract

The invention discloses low molecular weight protein which is produced from [beta]-carotene fermentation waste liquid, so that resource waste is reduced, and meanwhile, the problem of environmental pollution caused by the fermentation liquid is well solved; and a good application prospect is guaranteed. Meanwhile, a method provided by the invention is low in raw material cost, simple in equipment,convenient to operate, easy for implementation of large-scale production and capable of improving added value of the industry.

Description

technical field [0001] The invention relates to a method for extracting protein from beta-carotene fermentation liquid, in particular to the production technology of collecting and crushing mycelium and separating and extracting protein. Background technique [0002] β-carotene (C40H56) is one of the carotenoids and is also an orange-yellow fat-soluble compound. It is the most ubiquitous and stable natural pigment in nature. Many natural foods such as: green vegetables, sweet potatoes, carrots, spinach, papaya, mango, etc., are rich in β-carotene. β-carotene is an antioxidant with detoxification effect. It is an indispensable nutrient for maintaining human health. It has significant functions in anti-cancer, prevention of cardiovascular diseases, cataracts and anti-oxidation, and then prevents aging and aging-induced diseases. Various degenerative diseases. [0003] The applications of β-carotene include: 1. Mainly used in food, health care products and medicines; 2. Edibl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 韩萍王维张作进李冉张华黄鹏
Owner QINGDAO UNIV
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