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Vaccine composition containing porcine circovirus-2 antigen and swine flu antigen

A vaccine composition and a technology for porcine circovirus, which are applied in the field of swine vaccines, can solve problems such as the influence of swine immune function, the lack of prevention and treatment of swine influenza virus disease, and the reduction of swine growth performance.

Active Publication Date: 2014-03-26
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mutation rate of SIV is fast, the development of traditional vaccines is obviously lagging behind, the cross-protection ability is limited, and the ability to induce cellular immunity is poor, and the antibody production is slow (Cui Ke, Jiang Tian, ​​Research Progress of Swine Influenza Vaccine, Veterinary Guide, 2012 (02 ):53-54.)
[0004] The symptoms caused by swine flu and circovirus are basically the same, and swine flu is an conditionally pathogenic virus. When the two are mixed infection, it is easy for those skilled in the art to regard the mixed infection of the two as the treatment of swine circovirus and Prevention, resulting in the lack of control of swine influenza virus disease, resulting in reduced growth performance of pigs, mixed infection of swine influenza and circovirus also affects the immune function of pigs, and is very susceptible to severe infectious diseases such as swine fever and porcine blue ear disease

Method used

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  • Vaccine composition containing porcine circovirus-2 antigen and swine flu antigen
  • Vaccine composition containing porcine circovirus-2 antigen and swine flu antigen
  • Vaccine composition containing porcine circovirus-2 antigen and swine flu antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1. Isolation and Identification of Swine Influenza Strains

[0104] The isolation and identification of SIV H1N1 strain of the present invention and SIV H3N2 strain:

[0105] SIV H1N1 subtype virus (named ZJS strain) and SIV H3N2 subtype virus ( Named as WX strain), it was identified by RT-PCR identification, hemagglutination test and animal inoculation test.

[0106] SIV RT-PCR Identification Primers

[0107]

[0108] Such as figure 1 As shown, use the above SIV RT-PCR identification primers to identify SIV H1N1 and SIV H3N2: Lane M is Marker, A in lane 1 is the amplified band of the HA gene of the SIV H1N1 subtype ZJS strain, and B in lane 2 is The amplified band of the NA gene of the SIV H1N1 subtype ZJS strain, A in lane 3 is the amplified band of the HA gene of the SIV H3N2 subtype WX strain, and B in lane 4 is the amplified band of the NA gene of the SIV H3N2 subtype WX strain The amplified bands showed that the HA gene and NA gene of the two viruse...

Embodiment 2

[0116] Example 2: Preparation of porcine circovirus type 2 antigen and swine influenza virus antigen

[0117] Preparation of porcine circovirus type 2 (SH strain) virus solution: the PCV2 SH strain seed was mixed with 0.1 TCID 50 The inoculum amount per cell was inoculated on a monolayer of PK15 (purchased from ATCC, model ATCC CCL-33 TM ) cells, adsorb for 30 minutes at 37°C, add MEM liquid medium containing 4% (volume ratio) calf serum and 2mM D-glucosamine hydrochloride (prepared with MEM dry powder medium purchased from Life Technologies, USA, according to its instructions ), placed at 37°C to continue culturing. Observe twice a day, the cells grow well, harvest the cell culture after 4 days of culture at 37°C, freeze and thaw 3 times, harvest the virus, and use PBS to dilute the harvested virus liquid to a virus titer of 10 6.0 TCID 50 / mL, the batch number is 20110428.

[0118] Preparation of porcine circovirus type 2 (SH strain) subunit antigen: clone porcine circov...

Embodiment 3

[0121] Embodiment 3: treatment and inactivation of virus liquid or bacterial liquid.

[0122] Treatment of porcine circovirus type 2 (SH strain) virus liquid: filter the virus liquid prepared in Example 2 with batch number 20110428 through a hollow fiber (0.5 μm ~ 2 μm) filter column (purchased from GE Healthcare Life Sciences) to remove cell debris , and then add 0.2% to 0.3% formaldehyde solution of virus liquid volume to inactivate at 37°C for 18h. Porcine circovirus type 2 whole virus antigen was obtained, and the antigen content was that the virus titer before inactivation was 10 6.0 TCID 50 / ml, the batch number is 20110506.

[0123]Treatment of swine influenza virus liquid: filter the H1N1 subtype SIV antigen liquid with batch number 20110502 in Example 2 with a hollow fiber (0.5 μm ~ 2 μm) filter column (purchased from GE Healthcare Life Sciences) to remove cell debris, and then add 0.2% to 0.3% formaldehyde solution was inactivated at 37°C for 18 hours, and the bat...

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Abstract

The invention provides a vaccine composition containing porcine circovirus-2 antigen (PCV2) and swine flu antigen (SIV). The vaccine composition comprises at least one porcine circovirus-2 antigen of effective dose and at least one swine flue antigen of effective does as well as an adjuvant. The vaccine composition can be used for preventing and treating diseases associated to the porcine circovirus-2 and the swine flu virus diseases.

Description

technical field [0001] The invention relates to a vaccine for pigs, in particular to a vaccine against porcine circovirus type 2 (Porcine circovirus type 2, PCV2) and swine influenza virus (Swine influenza virus, SIV) infection simultaneously. Background technique [0002] Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus that is the main cause of multisystemic wasting syndrome (PMWS) in weaned piglets. The clinical features of PMWS include weight loss, pale skin, dyspnea, diarrhea, and jaundice. Pathological changes can be seen systemic lymph node enlargement, especially inguinal lymph node enlargement up to 5 to 10 times; the lungs mainly show diffuse and interstitial pneumonia changes, the texture is as hard as rubber, and the surface generally has a taupe mottled appearance; the kidney There are many changes, white necrotic lesions of dif...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N15/44C07K14/11A61K39/145A61K39/295A61K39/12A61P31/16A61P31/20C12R1/93
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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