Vaccine composition containing swine mycoplasma pneumonia antigen and porcine streptococcal disease antigen and its preparation method and application

A technology of mycoplasma pneumonia and vaccine composition, which is applied in the direction of antibacterial drugs and bacterial antigen components, which can solve the problems of drug resistance, stress and safety problems, residues, etc., to reduce the number of vaccinations, enhance the immune effect and effect better effect

Active Publication Date: 2016-03-09
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of antibacterial drugs often causes drug resistance and drug residue problems, and the treatment effect is not satisfactory
When using vaccines to prevent these two diseases, multiple injections of a single vaccine will cause stress and safety problems, and in the case of co-infection, the quality of the preventive effect will be reduced

Method used

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  • Vaccine composition containing swine mycoplasma pneumonia antigen and porcine streptococcal disease antigen and its preparation method and application
  • Vaccine composition containing swine mycoplasma pneumonia antigen and porcine streptococcal disease antigen and its preparation method and application
  • Vaccine composition containing swine mycoplasma pneumonia antigen and porcine streptococcal disease antigen and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, preparation of Mycoplasma hyopneumoniae antigen, Streptococcus suis antigen

[0039] 1. Source of bacteria (virus) strains

[0040] The selected Mycoplasma hyopneumoniae (Mhp for short) is HN0613 strain, the preservation number is CCTCCM2012230, and the preservation date is June 13, 2012.

[0041] The selected Streptococcus suis Type 2 (SS2 for short) is an SC strain, the preservation number is: CCTCCM2011351, and the preservation date is October 13, 2011.

[0042] The selected S. equisubsp. zooepidemicus (SESZ) strain is ATCC35246 strain (purchased from American type culture collection, namely American type culture collection, ATCC)

[0043] 2. Preparation and inspection of vaccine semi-finished products

[0044] 2.1 Preparation of seeds for production

[0045] 2.1.1 Streptococcus suis type 2, Streptococcus equi zooepidemic subspecies:

[0046] Propagation of primary seeds

[0047] Dilute the SS2SC strain and SESZATCC35246 freeze-dried strain with ...

Embodiment 2

[0073] Example 2. Preparation of vaccine composition

[0074] 1 Preparation of preservatives

[0075] 1% (w / v) thimerosal (Sinopharm Chemical Reagent Co., Ltd. 20120106) aqueous solution

[0076] 2 preparation of diluent

[0077] Sterile PBS (0.01M, pH=0.02) buffer solution: dissolve 8g sodium chloride, 0.25g potassium chloride, 3.63g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate in 900ml purified water, then dilute to 1L, autoclave at 121°C for 30 minutes for later use.

[0078] 3 preparation of adjuvants

[0079] Gel01 (French SEPPIC company 20120401), autoclaved at 121°C for 30 minutes for later use.

[0080] 4 with seedlings

[0081] Get the concentrated inactivated antigen of Mhp, SS2, SESZ prepared in Example 1, make mixed antigen solution or directly make antigen solution according to the final antigen content in combined vaccine and single vaccine, then antigen solution and Gel01 adjuvant by 90: 10 (v / v) mixed, supplemented to the required vol...

Embodiment 3

[0106] Example 3. This study is to evaluate the combination efficacy of Mhp-SS2-SESZ combination vaccines with different antigen contents.

[0107] 1 Material:

[0108] Embodiment 2 prepares vaccine:

[0109] Table 12

[0110]

[0111]

[0112] Animals: 4-5 weeks old, pigs with negative serum antibodies to Mhp, SS2, and SESZ

[0113] Challenge strains: SS2SC strain, SESZATCC35246 strain (purchased from American type culture collection, ATCC), MhpCVCC354 strain (purchased from China Veterinary Drug Control Institute)

[0114] 2 methods:

[0115] 50 piglets aged 4-5 weeks were selected and randomly divided into 10 groups, 5 piglets in each group. On the 0th day, each pig in groups 1, 4, and 7 was injected with vaccine L intramuscularly in the neck, and each pig in groups 2, 5, and 8 was injected with vaccine H in the neck. Intramuscular injection of sterile PBS, both 2ml / head, the 10th group was used as blank control, neither immunized nor challenged. The 1st to 6th ...

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Abstract

The invention provides a vaccine composition containing a swine mycoplasmal pneumonia antigen and a swine streptococcosis antigen, and a preparation method and an application thereof. The vaccine composition includes an immunizing dose of the swine mycoplasmal pneumonia antigen, an immunizing dose of the swine streptococcosis antigen, and an adjuvant. The vaccine composition has a simple immunization program, can effectively control the swine mycoplasmal pneumonia antigen and the swine streptococcosis antigen, has an immune effect equivalent to the immune effect realized through respective injection of single vaccines, and also has the characteristics of small side reaction, long immune period, less time consumption, and less labor consumption; and the vaccine composition also has the advantages of simple production technology, low immune cost and strong practicality.

Description

technical field [0001] The invention relates to a vaccine composition containing swine mycoplasma pneumonia antigen and suis streptococcosis antigen and a preparation method thereof, belonging to the field of veterinary medicine. Background technique [0002] Swine asthma, also known as swine mycoplasma pneumonia or swine endemic pneumonia, is a contact chronic respiratory infectious disease caused by Mycoplasma hyopneumoniae, which is prevalent all over the world. The diseased pigs mainly showed cough and wheezing, growth retardation, low feed conversion rate, and normal body temperature. During the anatomy, the main lesions were in the lungs, especially the pancreatoid changes and fleshy changes in the heart lobes, middle lobes and apical lobes of the two lungs. High morbidity and low mortality. Studies in recent years have found that the mixed infection of Mycoplasma hyopneumoniae with porcine reproductive and respiratory syndrome virus and other pathogens further incre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/09A61K39/02A61P31/04
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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