Vaccine composition containing porcine circovirus type 2 antigen and Haemophilus parasuis antigen and its preparation method and application

A technology of Haemophilus suis and porcine circovirus, which is applied in biochemical equipment and methods, medical preparations containing active ingredients, and microorganism-based methods, etc., can solve the problems of immunization failure, insufficient time interval, and increase the cost of epidemic prevention.

Active Publication Date: 2016-02-03
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bao Chunxing et al. "Analysis of the Causes and Countermeasures of Vaccine Immunization Failure" (Animal Husbandry and Veterinary Science and Technology Information 2010, (02) 8-12), reported that the interval between the two vaccinations is not enough, or some farmers have cumbersome epidemic prevention tasks. In order to save trouble, When the two vaccines are inoculated at the same time, after the vaccine enters the body, there may be competitive inhibition between the vaccine antigens, resulting in immune failure
In order to avoid immunization failure, pig farms generally adopt the strategy of immunizing PCV2 vaccine and Haemophilus parasuis vaccine separately. The time interval between the two vaccines is more than 15 days to avoid interference between the two antigens, which causes multiple Repeated injections increase animal stress and increase the cost of epidemic prevention, and the effect of epidemic prevention is not accurate

Method used

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  • Vaccine composition containing porcine circovirus type 2 antigen and Haemophilus parasuis antigen and its preparation method and application
  • Vaccine composition containing porcine circovirus type 2 antigen and Haemophilus parasuis antigen and its preparation method and application
  • Vaccine composition containing porcine circovirus type 2 antigen and Haemophilus parasuis antigen and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] The preparation and inspection of the vaccine composition of embodiment 1 porcine circovirus-related diseases and Haemophilus parasuis disease

[0089] 1.1 Preparation of bacteria (virus) species for production

[0090] Preparation of porcine circovirus type 2 SH: the virus seed PCV2SH strain was diluted 1:9 with MEM liquid medium (prepared with MEM dry powder medium purchased from Invitrogen Company of the United States according to the instructions), and then divided into 5% of the volume of cell culture medium. % inoculated in PK15 (ATCC, preservation number CCL-33) monolayer cells, adsorbed at 37°C for 30 minutes, added cell maintenance solution (4% calf serum and 2mmol / L D-glucosamine hydrochloride were added to MEM liquid medium) ), cultivated at 37°C for 4 days, freeze-thawed 2-3 times, harvested the virus, and the virus titer was 10 6.5 TCID 50 / ml.

[0091]Preparation of Haemophilus parasuis strains: Streak inoculation of Haemophilus parasuis type 4 JS strai...

Embodiment 2

[0149] Example 2: Preparation of porcine circovirus type 2 antigen and Haemophilus parasuis bivalent (serotype 4 + serotype 5) antigen vaccine composition

[0150] Prepared by Example 1 (i.e. K001 batch of antigens, see Table 2) porcine circovirus type 2 inactivated virus liquid and Haemophilus parasuis (serum type 4 JS strain and serum type 5 ZJ strain) inactivated bacterial liquid, Prepare the vaccine according to the following formula:

[0151] Inactivated PCV2 virus liquid (the virus content before inactivation is 10 6.0 TCID 50 / ml) 45ml

[0152] Inactivated Haemophilus parasuis type 4 bacteria liquid (the content of the bacteria liquid before inactivation is 4.5×10 9.0 cfu / ml) 22.5ml

[0153] Inactivated Haemophilus parasuis type 5 bacteria liquid (the content of the bacteria liquid before inactivation is 4.5×10 9.0 cfu / ml) 22.5ml

[0154] Montanide TM Gel10ml

[0155] Put the inactivated virus liquid and the inactivated bacterial liquid (prepared in Example 1) i...

Embodiment 3

[0156] Example 3: Comparison of PCV2 inactivated whole virus antigen vaccine against PCV2 challenge immune effect with PCV2 inactivated virus antigen and Haemophilus parasuis inactivated antigen vaccine composition

[0157] The antigens prepared in Example 1 (i.e. the K001 batch of antigens, see Table 2) were used to prepare porcine circovirus type 2, Haemophilus parasuis (serotype 4 + serotype 5) vaccine compositions (V1) and (V2), respectively. Porcine circovirus vaccine (V3) and (V4), the specific formula is as follows.

[0158] (1) Porcine circovirus type 2, Haemophilus parasuis (serotype 4 + serotype 5) vaccine composition (V1)

[0159] Inactivated PCV2 virus liquid (containing 10 6.0 TCID 50 / ml) 45ml

[0160] Inactivated Haemophilus parasuis type 4 bacteria liquid (containing 4.5×10 9.0 cfu / ml) 22.5ml

[0161] Inactivated Haemophilus parasuis type 5 bacteria liquid (containing 4.5×10 9.0 cfu / ml) 22.5ml

[0162] Montanide TM Gel10ml

[0163] (2) Porcine circovir...

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Abstract

The invention relates to a polyvalent vaccine composition for porcine, and in particular relates to a vaccine composition capable of resisting infection of porcine circovirus type 2 (PCV 2) and haemophilus parasuis (HPS) at the same time. The vaccine composition comprises at least one PCV2 antigen, at least one HPS, as well as vector, an excipient and an adjuvant available in the field of veterinary medicine. The vaccine composition can be used for preventing and treating PCV2 related diseases and HPS diseases.

Description

technical field [0001] The invention relates to a vaccine composition for pigs, in particular to a vaccine composition for simultaneously resisting porcine circovirus type 2 (PCV2) and Haemophilus parasuis (HPS) infection, a preparation method and application thereof. Background technique [0002] Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus that is the main cause of multisystemic wasting syndrome (PMWS) in weaned piglets. The clinical features of PMWS include weight loss, pale skin, dyspnea, diarrhea, and jaundice. Pathological changes can be seen systemic lymph node enlargement, especially inguinal lymph node enlargement up to 5 to 10 times; the lungs mainly show diffuse and interstitial pneumonia changes, the texture is as hard as rubber, and the surface generally has a taupe mottled appearance; the kidney There are many changes, whit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/20A61P31/04C12N1/20C12R1/21
Inventor 习向锋张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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