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Anti-porcine atrophic rhinitis and haemophilus parasuis vaccine composition and its preparation

A vaccine composition, the technology of Haemophilus suis, is applied in the field of animal husbandry biopharmaceuticals, and can solve the problems of inability to protect pigs, asynchronous immunity, etc.

Active Publication Date: 2016-08-31
PULIKE BIOLOGICAL ENG INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The asynchronous immunity of the two causes mixed infection and cannot effectively protect the pigs comprehensively and effectively!

Method used

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  • Anti-porcine atrophic rhinitis and haemophilus parasuis vaccine composition and its preparation
  • Anti-porcine atrophic rhinitis and haemophilus parasuis vaccine composition and its preparation
  • Anti-porcine atrophic rhinitis and haemophilus parasuis vaccine composition and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Preparation of porcine bronchiseptica Bordetella antigen, Pasteurella multocida antigen and Haemophilus parasuis antigen

[0062] 1. Source of the strain

[0063] Bordetella bronchiseptica HN8 strain (CCTCC NO: M2011223), Pasteurella multocida type A HN5 strain (CCTCC NO: M2011222) and D type HB4 strain (CCTCC NO: M2011221) used in the manufacture and inspection of this product, Haemophilus parasuis type 4 JS strain (CCTCC M 2011172) and type 5 ZJ strain (CCTCC M 2011173) were both isolated and identified by Pulaike Bioengineering Co., Ltd. Preserved by the Culture Collection Center.

[0064] The strains used in the manufacture and inspection of this product are Bordetella bronchiseptica HN8 strain, Pasteurella multocida type A HN5 strain and D type HB4 strain, Haemophilus parasuis type 4 JS strain and type 5 ZJ strain, All were stored freeze-dried.

[0065] 2. Preparation and inspection of vaccine semi-finished products

[0066] (1) Preparation of seeds ...

Embodiment 2

[0088] Embodiment 2: Preparation of anti-porcine atrophic rhinitis and Haemophilus parasuis disease vaccine composition

[0089] 1. Preparation of preservatives

[0090] 1% (w / v) thimerosal aqueous solution: 1 g of thimerosal was dissolved in 100 ml of purified water, and autoclaved at 121° C. for 30 minutes for later use.

[0091] 2. Preparation of diluent

[0092] Sterile PBS buffer solution: Dissolve 8g sodium chloride, 0.25g potassium chloride, 3.63g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate in 900ml purified water, then dilute to 1L, autoclave at 121°C for 30min spare.

[0093] 3. Vaccine Adjuvant Treatment

[0094] Sterilization of the Gel adjuvant: transfer the Gel adjuvant into a sterilizable container, and autoclave at 121°C for 30 minutes for later use.

[0095] 4. Matching seedlings

[0096] Mix the above ingredients in a certain proportion, that is, through aseptic operation, the concentrated antigen of Bordetella bronchiseptica, the co...

Embodiment 3

[0100] Example 3: Anti-porcine atrophic rhinitis and Haemophilus parasuis vaccine composition efficacy test with different antigen contents

[0101] 1. Test material

[0102] According to embodiment 2 laboratory preparation anti-porcine atrophic rhinitis and Haemophilus parasuis vaccine composition, vaccine 1 (bordetella porcine bronchiseptica antigen content 10 10 CFU / ml, Pasteurella multocida type A antigen content 10 10 CFU / ml, Pasteurella multocida type D antigen content 10 10 CFU / ml, Haemophilus parasuis type 4 antigen content 10 10 CFU / ml, Haemophilus parasuis type 5 antigen content 10 10 CFU / ml) and vaccine 2 (Bordetella porcine bronchiseptica antigen content 10 8 CFU / ml, Pasteurella multocida type A antigen content 10 8 CFU / ml, Pasteurella multocida type D antigen content 10 8 CFU / ml, Haemophilus parasuis type 4 antigen content 10 8 CFU / ml, Haemophilus parasuis type 5 antigen content 10 8 CFU / ml).

[0103] Weaned piglets aged 3 to 4 weeks without antibodies to...

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Abstract

The invention relates to an anti-atrophic rhinitis and haemophilus parasuis vaccine composition and a preparation method thereof. The vaccine composition contains components such as a pig bordetella bronchiseptica antigen, a pig toxigenic pasteurellamultocida antigen and a haemophilus parasuis antigen, wherein a bacterium solution of each antigen component is obtained by a fermentation cultivation method, and is concentrated by an ultrafiltration concentration technology. According to the vaccine composition, the antigens in the vaccine composition are high in content and comprehensive, so that animals can be comprehensively and effectively protected; a hydrogel adjuvant is adopted, so that the vaccine composition can be well absorbed, and is quick in immune response, a local immune part is free of adverse reaction, and the immune protection of pigs can be improved; the problem of effective and comprehensive protection of the pigs during mixed infection is effectively solved, immunization procedures are simplified, the effect of preventing multiple diseases by once injection is achieved, side effects caused by multiple immunization are reduced, the immunization cost is lowered, and the vaccine composition is economical and practical.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry biopharmaceuticals, and relates to a vaccine composition against porcine atrophic rhinitis and Haemophilus parasuis disease and a preparation method thereof. Background technique [0002] Porcine atrophic rhinitis (atrophic rhinitis, AR) is an important respiratory infectious disease in pigs. It can cause symptoms such as chronic rhinitis, sneezing, tearing, facial degeneration and turbinate atrophy in pigs. It can cause snout deformation and affect the bone development of the whole body, thereby causing growth retardation of pigs, making pigs susceptible to secondary complex pneumonia, and causing huge losses to the pig industry. The disease can infect pigs of any age, and the pigs under 4 weeks of age have the highest infection rate, and symptoms generally appear at 8 to 12 weeks of age. Porcine atrophic rhinitis can be divided into progressive atrophic rhinitis (PAR) and nonprogressiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/116A61K39/295A61P11/02A61P31/04A61K39/02A61K39/10A61K39/102A61K39/12A61K39/145A61K39/187A61K39/245
Inventor 张许科孙进忠白朝勇
Owner PULIKE BIOLOGICAL ENG INC
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