Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vaccine composition, preparation method and application thereof

A vaccine composition and composition technology, applied in the field of vaccine composition, can solve problems such as inability to vaccine immune resistance, drug-resistant drugs, and decline in the quality of immune prevention effects

Active Publication Date: 2014-12-31
PU LIKE BIO ENG
View PDF11 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is still impossible to achieve a vaccine against the mixed infection caused by these three pathogens.
[0006] For the immunization of diseases caused by multi-pathogen mixed infection, multiple injections are required when using a single vaccine for immunization. Since the use of antibacterial drugs often causes drug resistance and drug residue problems, multiple injections of a single vaccine will not only cause stress and safety problems , and in the case of co-infection, the quality of immunoprophylaxis decreases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vaccine composition, preparation method and application thereof
  • Vaccine composition, preparation method and application thereof
  • Vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The preparation of embodiment 1 mycoplasma hyopneumoniae antigen, swine influenza virus antigen and porcine blue ear virus antigen

[0052] 1. Preparation of Mycoplasma hyopneumoniae antigen

[0053] 1.1 Source of the strain:

[0054] The strain of Mycoplasma hyopneumoniae used in the manufacture and inspection of this product is HN0613, which was preserved in the China Center for Type Culture Collection (abbreviation: CCTCC; address: Wuhan University, Wuhan, China). The preservation date: June 13, 2012, and the preservation number: CCTCC NO: M2012230.

[0055] 1.2 Preparation of seeds for Mycoplasma hyopneumoniae production:

[0056] Propagation of primary seeds: freeze-dried strain (HN0613 strain, preservation number CCTCC No.M2012230), diluted with liquid medium, streaked and inoculated on a solid medium plate, cultured at 37°C for 7 days, and selected well-growing colonies , inoculated on the slant of solid medium, cultivated at 37°C for 7 days, and used as first...

Embodiment 2

[0076] The preparation of embodiment 2 mycoplasma pneumonia, swine influenza and porcine PRRS triple vaccine

[0077] 1. Preparation of parts of mycoplasma swine pneumonia and swine influenza inactivated vaccines

[0078] 1.1 Preparation of diluent

[0079] Sterile PBS buffer solution: Dissolve 8g sodium chloride, 0.25g potassium chloride, 3.63g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate in 900ml purified water, then dilute to 1L, autoclave at 121°C for 30min spare.

[0080] 1.2 Vaccine adjuvant treatment

[0081] Sterilization of Gel 01 adjuvant: transfer the Gel 01 adjuvant into a sterilizable container, and autoclave at 121°C for 30 minutes for later use.

[0082] 1.3 Matching seedlings

[0083] Through aseptic operation, the concentrated Mycoplasma hyopneumoniae antigen and swine influenza virus antigen prepared in Example 1 were mixed with adjuvants, preservatives, diluents, etc. according to different proportions, and stirred at a low speed by ...

Embodiment 3

[0090] Example 3 Efficacy Test of Triple Vaccine of Mycoplasma Swine Pneumonia, Swine Influenza and Pig PRRS with Different Antigen Contents

[0091] 1 test material

[0092] Vaccine 1 (mycoplasma hyopneumoniae 10) prepared in embodiment 2 7 MHDCE / toufen, swine influenza virus (H1N1 subtype) and swine influenza virus (H3N2 subtype) each 10 4 EID 50 / Toufen, porcine blue ear virus 10 4 TCID 50 / head), vaccine 2 (Mycoplasma hyopneumoniae 10 8 MHDCE / toufen, swine influenza virus (H1N1 subtype) and swine influenza virus (H3N2 subtype) each 10 5 EID 50 / Toufen, porcine blue ear virus 10 5 TCID 50 / head), vaccine 3 (Mycoplasma hyopneumoniae 10 9 MHDCE / toufen, swine influenza virus (H1N1 subtype) and swine influenza virus (H3N2 subtype) each 10 6 EID 50 / Toufen, porcine blue ear virus 10 6 TCID 50 / head), vaccine 4 (Mycoplasma hyopneumoniae 10 10 MHDCE / toufen, swine influenza virus (H1N1 subtype) and swine influenza virus (H3N2 subtype) each 10 7 EID 50 / Toufen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a vaccine composition, which includes an immune amount of a mycoplasma hyopneumoniae antigen, an immune amount of a swine influenza virus antigen and an immune amount of a porcine reproductive and respiratory syndrome virus antigen. All antigens of the vaccine composition not only do not generate mutual interference or influence of antigen components, but have the effect of mutually enhancing the immune effect instead. One immunization can achieve the immune effect and the antibody continuous level of single antigen twice immunization. Also, the vaccine composition has the advantages of good security, simple preparation method, convenient and fast immunization, and reduction of the immunization cost, etc.

Description

technical field [0001] The invention relates to a vaccine composition, a preparation method and application of the vaccine composition. Background technique [0002] Porcine Reproductive and Respiratory Syndrome (PRRS), also known as porcine blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus, PRRSV) and is characterized by fever and abortion in sows. , increased mortality of piglets before and after weaning, and respiratory disorders of pigs of different ages are clinically characteristic diseases. Porcine reproductive and respiratory syndrome virus, also known as porcine blue ear virus, is a single-stranded positive-sense RNA virus with an envelope. The virus has two serotypes, namely the American type and the European type. At present, the disease is one of the important diseases seriously affecting the development of the pig industry in the world. Vaccination is the most effective way t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/14A61P31/16A61K39/12A61K39/145A61K39/02
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com