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Swine fever and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof

A technology of porcine pseudorabies and porcine pseudorabies virus, which is applied in antiviral agents, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problem of the immune effect of the double vaccine on porcine challenge, and the source of the virus is not described and cannot be seen. To solve problems such as swine fever and porcine pseudorabies dual vaccine, two kinds of antigen interference, etc., to achieve good immune protection, convenient and fast immunization, and reduce the number of immunizations

Inactive Publication Date: 2014-01-15
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Chinese patent CN 101690808A also tries to use the dual vaccine of classical swine fever and pseudorabies, the dual vaccine requires at least each head containing CSF virus to be greater than 500,000 rabbits, and the PRV is greater than 10 8 TCID 50 , it is necessary to prepare a large amount of high-titer virus liquid, and its commercial utilization is very small, and the immune effect of the double vaccine against pig challenge and the source of the PR virus have not been described
Although the author has also prepared a dual vaccine of classical swine fever and porcine pseudorabies, in order to overcome the interference between the two antigens, transfer factor needs to be added, which has certain side effects, which is not conducive to the application in production practice
Therefore, although people have always hoped to obtain a safe, reliable, and good immune effect dual vaccine against swine fever and porcine pseudorabies, due to the interference between the two antigens, it has not been possible to see a swine fever vaccine that meets the requirements and is suitable for industrial production. Combination Vaccine with Porcine Pseudorabies

Method used

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  • Swine fever and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof
  • Swine fever and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof
  • Swine fever and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 swine fever, porcine pseudorabies dual live vaccine

[0037] 1. Preparation of classical swine fever and porcine pseudorabies virus antigens

[0038] 1.1 Preparation of poisonous seeds for production

[0039] The preparation of the attenuated strain of classical swine fever rabbitization: the attenuated strain of classical swine fever rabbitization (purchased from the China Veterinary Drug Administration, the preservation number is AV1412) is properly diluted with the virus diluent (DMEM medium without serum), according to the multiplicity of infection ( M.O.I) was 0.1, inoculated on ST cell monolayer (purchased from CCTCC, No. GDC0060) and cultured, adsorbed at 37°C for 30 minutes, added DMEM cell maintenance solution containing 3% (v / v) calf serum, and cultured at 37°C for 3 to 5 days , freeze and thaw 2 to 3 times, harvest virus, virus titer ≥ 10 6.5 TCID 50 / ml.

[0040] Preparation of porcine pseudorabies virus gene deletion stra...

Embodiment 2

[0081] Antibody effect comparison after embodiment 2 swine fever, porcine pseudorabies dual live vaccine immunized piglets

[0082] 1. Materials

[0083] Swine fever, porcine pseudorabies dual live vaccine, select the vaccine product in embodiment 1 (lot number is trial Z001); The content is the same as that of the dual live vaccine, which is 1.0×10 4.0 TCID 50 / head part; Porcine pseudorabies live vaccine (porcine pseudorabies single vaccine), the porcine pseudorabies virus antigen in the embodiment 1 is selected, and its virus content is identical with the double live vaccine, is 1.0 * 10 6.0 TCID 50 / Touquan.

[0084] 2. Design of animal experiments

[0085] A total of 35 21-day-old piglets were selected and divided into 4 groups. Groups 1 to 3 were the experimental groups with 10 pigs / group, and group 4 was the control group (5 pigs / group). In the first group, each pig was intramuscularly injected with 1ml of swine fever and porcine pseudorabies dual live vaccine (b...

Embodiment 3

[0096] Example 3 Comparing the protective effect of swine fever and porcine pseudorabies dual live vaccine immunizing piglets with virulent strains of swine fever

[0097] 1. Materials

[0098] Swine fever, porcine pseudorabies dual live vaccine, select the vaccine product in embodiment 1 (lot number is trial Z001); Same, 1.0×10 4.0 TCID 50 / Touquan.

[0099] 2. Design of animal experiments

[0100] Fifteen 21-day-old piglets were selected and divided into 3 groups, 5 piglets per group. Groups 1 and 2 were the test group, and group 3 was the control group. Each pig in the first group was intramuscularly injected with 1ml of swine fever and porcine pseudorabies live vaccine (batch number 001) in the neck, each pig in the second group was injected with 1ml of the live vaccine against swine fever in the neck, and the third group was not vaccinated. 14 to 10 after immunization 5.0 The minimal lethal dose (MLD) of the virulent strain of CSF Shihmen virus was challenged for 16 c...

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Abstract

The invention provides a swine fever and porcine pseudorabies bivalent vaccine. The swine fever and porcine pseudorabies bivalent vaccine contains at least one swine fever virus antigen and at least one porcine pseudorabies virus antigen, wherein the two antigens coordinate well, are excellent in immune effect and can promote each other. The swine fever and porcine pseudorabies bivalent vaccine is simple in preparation method, is convenient and efficient in immunization and has the advantages that immunization cost is reduced, an immunization procedure is simplified and economy and reliability are realized compared with a vaccine which can be used for preventing and treating more than two diseases only when immunization is carried out in steps and at least two injections are taken and an immune method of the vaccine in the prior art. The immune effect of the swine fever and porcine pseudorabies bivalent vaccine is better than that of a single vaccine and better in safety and avoids adverse effects caused by multiple immunizations. Besides, the invention also provides a simple testing method for determining swine fever effect in the bivalent vaccine by adopting an indirect immunofluorescence method, so that quality of bivalent live vaccines in each batch is guaranteed, and economic benefit is obviously increased.

Description

technical field [0001] The invention relates to a dual vaccine of swine fever and porcine pseudorabies, in particular to a dual vaccine of swine fever and porcine pseudorabies formulated in a specific ratio. Background technique [0002] Classical Swine fever (CSF for short), also known as hog cholera (Hog cholera) or European swine fever (European swine fever), is a viral infectious disease with acute or hyperacute fever in pigs, clinically characterized Is rapid dissemination, fever, typical hemorrhagic lesions can be seen on autopsy. Swine fever can infect pigs of all ages and is prevalent throughout the year with high morbidity and mortality, which is extremely harmful to pigs. This disease is one of the most important infectious diseases threatening the pig industry. The World Organization for Animal Health (OIE) lists swine fever as one of the 16 notifiable infectious diseases of category A, and China defines it as a severe infectious disease. Pseudorabies (PR) is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/22A61K39/187A61K39/245
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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