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Antigen composition for preventing and treating secondary infected respiratory system diseases of pigs, preparation method and application thereof

A technology for respiratory diseases and secondary infection, applied in the field of veterinary vaccines, can solve problems such as high cost, decreased efficacy of live vaccines, and severe pig stress

Active Publication Date: 2014-05-14
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no combination vaccine of Haemophilus parasuis and PRRS in the market, or the combined use of the two at the same time. This is because the existence of inactivated Haemophilus parasuis vaccine will affect the immune effect of PRRS live vaccine. The possible reasons are as follows: first, the oily adjuvant used in the inactivated vaccine is too viscous to be used as a diluent to dilute the live vaccine; second, the toxic components contained in the inactivated vaccine: inactivating agents such as formaldehyde; preservatives such as thimerosal, Nitrogen and sodium; toxins such as a large amount of endotoxin, metabolites and miscellaneous proteins in the culture process have a significant impact on the toxicity of live vaccines and reduce the efficacy of live vaccines
Moreover, commercially available commercial vaccines need to be immunized multiple times, the cost is high, and the stress on pigs is relatively large

Method used

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  • Antigen composition for preventing and treating secondary infected respiratory system diseases of pigs, preparation method and application thereof
  • Antigen composition for preventing and treating secondary infected respiratory system diseases of pigs, preparation method and application thereof
  • Antigen composition for preventing and treating secondary infected respiratory system diseases of pigs, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention. Example 1 Large-scale Production of Porcine Reproductive and Respiratory Syndrome Virus and Preparation of Vaccine in Tidal Microcarrier Suspension Bioreactor

[0070] The microcarrier used in the present embodiment is polyester fiber, and the virus strain that is used to prepare reproductive and respiratory syndrome virus anti...

Embodiment 2

[0085] The screening of embodiment 2 adjuvants

[0086] Using Haemophilus parasuis serum type 4 JS strain, type 5 ZJ strain antigen, respectively with white oil adjuvant, 206 adjuvant, nano aluminum hydroxide gel adjuvant, Montanide IMS1313 VG, MontanideISA15A VG, Montanide GEL 01 PR and Montanide GEL ST polymer adjuvant was formulated separately, and the antigen content of the finished vaccine was 2.0×10 for the serum type 4 JS strain and type 5 ZJ strain before inactivation. 9 CFU / head serving. The prepared vaccine was tested for compatibility with the PRRS vaccine prepared in Example 1 after the character test, the safety test and the potency test.

[0087] (1) Trait test (see Table 2)

[0088] Table 2 Appearance, sterility test and viscosity determination of vaccines with various adjuvants

[0089]

[0090] (2) Safety: The prepared vaccine was injected intramuscularly into 5 healthy susceptible pigs, 4mL each. Within 14 days, some vaccine groups had local or system...

Embodiment 3

[0104] Embodiment 3 Haemophilus parasuis inactivated vaccine (JS strain+ZJ strain) preparation

[0105] Inactivated Haemophilus parasuis disease vaccine (JS strain + ZJ strain), which has been inactivated, ultra-filtered and passed the security inspection, uses Haemophilus parasuis serum type 4 JS strain, type 5 ZJ strain antigen, and MontanideGEL 01 ST The adjuvant is mixed and prepared, wherein the ratio of the above two antigens is 1:1, accounting for 90% of the total volume of the vaccine, the content of the adjuvant is 10%, and the antigen content of the finished vaccine is the serum type 4 JS strain and 5 ZJ strain before inactivation. The number of live bacteria was 2.1×10 9 CFU / head serving.

[0106] 1. Strains

[0107] 1.1 The JS strain of Haemophilus parasuis serotype 4 was isolated and identified by Pulaike Bioengineering Co., Ltd., and has been preserved in the China Center for Type Culture Collection. The preservation date is May 18, 2011, and the preservation...

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Abstract

The invention provides an antigen composition for preventing and treating secondary infected respiratory system diseases of pigs. The antigen composition comprises at least one porcine reproductive and respiratory syndrome antigen with immune amount, and at leas one haemophilus parasuis antigen with immune amount and an adjuvant, wherein the porcine reproductive and respiratory syndrome antigen and the haemophilus parasuis antigen respectively and independently comprise attenuated live holoantigen, inactivated holoantigen, subunit antigen, recombinant live carrier antigen and DNA (deoxyribonucleic acid) carrier antigen. The antigen composition cannot cause mutual immune interference or influence of two antigen components, and enables the immune effects of porcine reproductive and respiratory syndrome antigen and the haemophilus parasuis antigen to be strengthened, and is convenient in vaccination process and good in safety.

Description

technical field [0001] The invention relates to a vaccine composition for preventing and treating Haemophilus parasuis and porcine reproductive and respiratory syndrome and a preparation method thereof, belonging to the field of veterinary vaccines. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine Reproductive and Respiratory Syndrome, PRRS), also known as porcine blue ear disease, is caused by single-stranded positive-sense RNA porcine reproductive and respiratory syndrome virus, and is characterized by reproductive disorders (abortion, stillbirth) in pregnant sows. , mummified fetuses) and pigs of various ages, especially piglets, are characterized by respiratory diseases, and have now become one of the main diseases in large-scale pig farms. The highly pathogenic porcine blue-ear disease that broke out in some areas of my country in 2006 is an acute and highly lethal disease caused by a mutant strain of the porcine reproductive and resp...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P31/04A61P11/00A61K39/12A61K39/102
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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