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Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen and preparation method and application thereof

A technology of Haemophilus suis and vaccine composition, applied in the field of vaccine composition for pigs, can solve the problems of complicated epidemic prevention tasks, increased animal stress, immune failure and the like

Active Publication Date: 2016-02-17
PULIKE BIOLOGICAL ENG INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Bao Chunxing et al. "Analyzed the Causes and Countermeasures of Vaccine Immunization Failure" (Animal Husbandry and Veterinary Science and Technology Information 2010, (02) 8-12), reported that the interval between the two vaccinations was not enough, or some farmers had cumbersome epidemic prevention tasks. In order to save trouble, When the two vaccines are inoculated at the same time, after the vaccine enters the body, there may be competitive inhibition between the vaccine antigens, resulting in immune failure
In order to avoid immunization failure, pig farms generally adopt the strategy of immunizing PCV2 vaccine and Haemophilus parasuis vaccine separately. The time interval between the two vaccines is more than 15 days to avoid interference between the two antigens, which causes multiple Repeated injections increase animal stress and increase the cost of epidemic prevention, and the effect of epidemic prevention is not accurate

Method used

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  • Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen and preparation method and application thereof
  • Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen and preparation method and application thereof
  • Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] The preparation and inspection of the vaccine composition of embodiment 1 porcine circovirus-related diseases and Haemophilus parasuis disease

[0090] 1.1 Preparation of bacteria (poison) species for production

[0091] The preparation of porcine circovirus type 2 SH: the virus seed PCV2SH strain was diluted 1:9 with MEM liquid medium (prepared according to the instruction manual with the MEM dry powder medium purchased from Invitrogen Company of the United States), and then diluted by 5% of the volume of the cell culture medium. % inoculated on PK15 (ATCC, preservation number is CCL-33) monolayer cells, adsorbed at 37°C for 30 minutes, added cell maintenance solution (add 4% calf serum and 2mmol / L D-glucosamine hydrochloride in MEM liquid medium) ), cultured at 37°C for 4 days, frozen and thawed 2 to 3 times, and the virus was harvested, and the virus titer was 10 6.5 TCID 50 / ml.

[0092]Preparation of Haemophilus parasuis strains: Streak inoculation of Haemophilu...

Embodiment 2

[0151] Embodiment 2: the preparation of porcine circovirus type 2 antigen, Haemophilus parasuis bivalent (serum type 4+serum type 5) antigen vaccine composition

[0152] Adopt embodiment 1 to prepare (i.e. K001 batch of antigens, see Table 2) porcine circovirus type 2 inactivated virus liquid and the inactivated bacterial liquid of Haemophilus parasuis (serum type 4 JS strain and serum type ZJ strain), Prepare the vaccine according to the following formula:

[0153] Inactivated PCV2 virus liquid (the virus content before inactivation is 10 6.0 TCID 50 / ml) 45ml

[0154] Inactivated Haemophilus parasuis type 4 bacteria liquid (the content of the bacteria liquid before inactivation is 4.5×10 9.0 cfu / ml) 22.5ml

[0155] Inactivated Haemophilus parasuis type 5 bacteria liquid (the content of the bacteria liquid before inactivation is 4.5×10 9.0 cfu / ml) 22.5ml

[0156] Montanide TM Gel10ml

[0157] Put the inactivated virus liquid and the bacterium liquid (prepared in embod...

Embodiment 3

[0158] Example 3: Comparison of PCV2 inactivated whole virus antigen vaccine against PCV2 challenge immune effect with PCV2 inactivated virus antigen and Haemophilus parasuis inactivated antigen vaccine composition

[0159] Antigens prepared in Example 1 (i.e. K001 batch of antigens, see Table 2) were used to prepare porcine circovirus type 2, Haemophilus parasuis (serum type 4+serum type 5) vaccine compositions (V1) and (V2), respectively Porcine circovirus vaccine (V3) and (V4), the specific formula is as follows.

[0160] (1) Porcine circovirus type 2, Haemophilus parasuis (serotype 4 + serotype 5) vaccine composition (V1)

[0161]

[0162] (2) Porcine circovirus type 2, Haemophilus parasuis (serotype 4 + serotype 5) vaccine composition (V2) total volume 100ml

[0163] Inactivated PCV2 virus solution (containing 10 6.0 TCID 50 / ml) 22.5ml

[0164] Inactivated Haemophilus parasuis type 4 bacterial liquid (containing 4.5×10 9.0 cfu / ml) 11.25ml

[0165] Inactivated Ha...

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Abstract

The invention relates to a multi-vaccine composition for pigs, in particular to a vaccine composition resisting porcine circovirus type 2 (PCV2) infection and haemophilus parasuis (HPS) infection at the same time. The vaccine composition is prepared from at least one kind of a porcine circovirus type 2 antigen, at least one kind of a haemophilus parasuis antigen, carriers, excipients and adjuvants, wherein the carriers, the excipients and the adjuvants can be obtained in the veterinary medicine field. The vaccine composition can be used for preventing and treating porcine circovirus type 2 related diseases and haemophilus parasuis diseases.

Description

[0001] This application is a divisional application of patent application 201210180842.0 technical field [0002] The invention relates to a vaccine composition for pigs, in particular to a vaccine composition for simultaneously resisting porcine circovirus type 2 (PCV2) and Haemophilus parasuis (HPS) infection, a preparation method and application thereof. Background technique [0003] Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus, which is the main pathogen of multisystemic wasting syndrome (PMWS) in weaned piglets. The clinical features of PMWS include weight loss, pale skin, dyspnea, diarrhea, and jaundice. Pathological changes can be seen systemic lymph node enlargement, especially inguinal lymph node enlargement up to 5 to 10 times; the lungs mainly show diffuse and interstitial pneumonia changes, the texture is as hard as rubber, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/20A61P31/04C12N1/20C12R1/21
Inventor 张许科孙进忠白朝勇
Owner PULIKE BIOLOGICAL ENG INC
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