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Porcine circovirus type II inactivated vaccine of and method for preparing same

A porcine circovirus and inactivated vaccine technology, applied in antiviral agents, virus antigen components, etc., can solve the problems of destroying the immune activity of PCV2 antigen, rough vaccine production process, and reduced immune effect, and achieve good immunogenicity and low cost. Low, infection prevention effect

Active Publication Date: 2009-10-07
兆丰华生物科技(南京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Low antigen concentration: the growth titer of PCV2 in cells is low, generally only 10 3 TCID 50 / ml, and vaccine production and antigen dose are the key to the large-scale application of PCV2 inactivated vaccines, so reducing production costs and improving vaccine quality and production have become thorny issues to be solved urgently;
[0007] (2) The vaccine production process is relatively rough: each virus has its own optimal production process, but in the preparation process of PCV2 inactivated vaccine, most people ignore the optimization and improvement of the production process, thus directly reducing or destroying the production process. Immunological activity of PCV2 antigens, resulting in reduced immune efficacy

Method used

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  • Porcine circovirus type II inactivated vaccine of and method for preparing same
  • Porcine circovirus type II inactivated vaccine of and method for preparing same

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Experimental program
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Effect test

Embodiment 1

[0032] The preparation of embodiment 1 porcine circovirus type II DBN-SX07 strain inactivated vaccine

[0033] (1) Preparation of poison for production

[0034] Get the required number of cells with a cell coverage rate of more than 80% and discard the growth medium, insert porcine circovirus type II DBN-SX07 strain virus seeds at a ratio of 5% (V / V), 37 After adsorption at ℃ for 1 hour, maintenance solution was added for incubation. After 24 hours, treat with 300mM D-glucosamine at 37°C for 20min, wash twice with sterile PBS (pH 7.4), add maintenance solution, rotate at 37°C for 48 hours, freeze and thaw three times, centrifuge or Cell debris was removed by filtration and frozen at -40°C.

[0035] (2) Inactivation of poisons used in production

[0036] Add the qualified virus solution to the formaldehyde solution at a ratio of 0.5‰ (V / V), mix well and inactivate at 37°C for 16 hours, shaking 4-5 times during the period.

[0037] (3) emulsification

[0038] The inactivate...

Embodiment 2

[0056] The preparation of embodiment 2 porcine circovirus type II inactivated vaccines

[0057] (1) Preparation of poison for production

[0058] Get the required number of cells with a cell coverage rate of more than 80% and discard the growth medium, insert porcine circovirus type II DBN-SX07 strain virus seeds at a ratio of 5% (V / V), 37 After adsorption at ℃ for 1 hour, maintenance solution was added for incubation. After 24 hours, treat with 300mM D-glucosamine at 37°C for 20min, wash twice with sterile PBS (pH 7.4), add maintenance solution, rotate at 37°C for 48 hours, freeze and thaw three times, centrifuge or Cell debris was removed by filtration and frozen at -40°C.

[0059] (2) Inactivation of poisons used in production

[0060] Add the qualified virus solution to the formaldehyde solution at a ratio of 0.75‰ (V / V), mix well and inactivate at 37°C for 20 hours, shaking 4-5 times during the period.

[0061] (3) emulsification

[0062] The inactivated vaccine is p...

Embodiment 3

[0066] The preparation of embodiment 3 porcine circovirus type II inactivated vaccines

[0067] (1) Preparation of poison for production

[0068] Get the required number of cells with a cell coverage rate of more than 80% and discard the growth medium, insert porcine circovirus type II DBN-SX07 strain virus seeds at a ratio of 5% (V / V), 37 After adsorption at ℃ for 1 hour, maintenance solution was added for incubation. After 24 hours, treat with 300mM D-glucosamine at 37°C for 20min, wash twice with sterile PBS (pH 7.4), add maintenance solution, rotate at 37°C for 48 hours, freeze and thaw three times, centrifuge or Cell debris was removed by filtration and frozen at -40°C.

[0069] (2) Inactivation of poisons used in production

[0070] Add the qualified virus solution to the formaldehyde solution at a ratio of 1‰ (V / V), mix well and inactivate at 37°C for 24 hours, shaking 4-5 times during the period.

[0071] (3) emulsification

[0072] The inactivated vaccine is prep...

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Abstract

The present invention belong to veterinary new biological medicine technology field, relates to porcine circovirus type II (PCV2) inactivated vaccine of and method for preparing same. The vaccine used seed virus is porcine circovirus type II DBN-SX07 strain, the preservation number is CGMCC No 3064, the virus strain is used as antigen preparation of inactivation by alkyl agents and emulsification by adding oil adjuvant. Using the invention provided PCV2 inactivated vaccine to immune pig can generate an uniform and effective protection force-shorting PCV2 infection windows period obviously, and prolong immune duration-reducing times of booster immunization.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biomedicine, and relates to an inactivated porcine circovirus type II vaccine and a preparation method thereof. Background technique [0002] Since 1991, various diseases such as multisystemic wasting syndrome (PMWS), porcine respiratory disease syndrome (PRDC), porcine skin and nephrotic syndrome (PDNS) caused by PCV2 infection (collectively known as porcine circovirus disease) , PCVDs) have caused huge losses to the global swine industry. The immunosuppression caused by viral infection can lead to the failure of existing vaccine immunity, resulting in mixed (or secondary) infection of multiple pathogens, and making the epidemic more serious. [0003] In the field, PMWS mainly occurs in weaned piglets. The pig herds show progressive emaciation, stunted growth, pale skin, enlarged lymph nodes and jaundice. The incidence rate is 3%-50%, and the mortality rate is 8%-35%. PRDC mainly It appe...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61P31/20
Inventor 邢海云赵建增赵亚荣
Owner 兆丰华生物科技(南京)有限公司
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