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Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen, as well as preparation method and application thereof

A technology of Haemophilus suis and vaccine composition, applied in the field of vaccine composition for pigs, can solve the problems of immune failure, increase of epidemic prevention cost, complicated epidemic prevention tasks and the like

Active Publication Date: 2013-03-27
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bao Chunxing et al. "Analysis of the Causes and Countermeasures of Vaccine Immunization Failure" (Animal Husbandry and Veterinary Science and Technology Information 2010, (02) 8-12), reported that the interval between the two vaccinations is not enough, or some farmers have cumbersome epidemic prevention tasks. In order to save trouble, When the two vaccines are inoculated at the same time, after the vaccine enters the body, there may be competitive inhibition between the vaccine antigens, resulting in immune failure
In order to avoid immunization failure, pig farms generally adopt the strategy of immunizing PCV2 vaccine and Haemophilus parasuis vaccine separately. The time interval between the two vaccines is more than 15 days to avoid interference between the two antigens, which causes multiple Repeated injections increase animal stress and increase the cost of epidemic prevention, and the effect of epidemic prevention is not accurate

Method used

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  • Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen, as well as preparation method and application thereof
  • Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen, as well as preparation method and application thereof
  • Vaccine composition containing porcine circovirus type 2 antigen and haemophilus parasuis antigen, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Preparation and inspection of vaccine composition for porcine circovirus related diseases and Haemophilus parasuis disease

[0089] 1.1 Preparation of bacterial (toxin) species for production

[0090] Preparation of porcine circovirus type 2 SH: Dilute the virus seed PCV2SH with MEM liquid medium (prepared with MEM dry powder medium purchased from Invitrogen in the United States according to the instructions) at 1:9, and then press 5 of the volume of the cell culture medium. % Inoculated into PK15 (ATCC, deposit number CCL-33) monolayer cells, adsorbed at 37°C for 30 minutes, and added cell maintenance solution (memory liquid medium with 4% calf serum and 2mmol / L D-glucosamine hydrochloride ), incubate at 37°C for 4 days, freeze and thaw 2~3 times, harvest the virus, the virus titer is 10 6.5 TCID 50 / ml.

[0091] Preparation of Haemophilus parasuis strains: Streak inoculation of Haemophilus parasuis type 4 JS strain and Haemophilus parasuis type 5 ZJ strain into 5%...

Embodiment 2

[0149] Example 2: Preparation of porcine circovirus type 2 antigen and Haemophilus parasuis bivalent (serotype 4 + serotype 5) antigen vaccine composition

[0150] The inactivated virus liquid of porcine circovirus type 2 and Haemophilus parasuis (serotype 4 JS strain and serotype 5 ZJ strain) were prepared using Example 1 (ie, K001 batch of antigen, see Table 2), Prepare the vaccine according to the following formula:

[0151] Inactivated PCV2 virus solution (the virus content before inactivation is 10 6.0 TCID 50 / ml)45ml

[0152] Inactivated Haemophilus parasuis type 4 bacterial liquid (the content of the bacterial liquid before inactivation is 4.5×10 9.0 cfu / ml) 22.5ml

[0153] Inactivated Haemophilus parasuis type 5 bacterial liquid (the content of the bacterial liquid before inactivation is 4.5×10 9.0 cfu / ml) 22.5ml

[0154] Montanide TM Gel 10ml

[0155] Put the inactivated virus liquid and the inactivated bacterial liquid (prepared in Example 1) into the container according to ...

Embodiment 3

[0156] Example 3: Comparison of the immune effect of PCV2 inactivated whole virus antigen vaccine against PCV2 challenge and the vaccine composition of PCV2 inactivated virus antigen and Haemophilus parasuis inactivated antigen against PCV2 challenge

[0157] Example 1 was used to prepare antigens (ie K001 batch of antigens, see Table 2) respectively to prepare porcine circovirus type 2 and Haemophilus parasuis (serotype 4 + serotype 5) vaccine compositions (V1) and (V2), Porcine circovirus vaccine (V3) and (V4), the specific formula is as follows.

[0158] (1) Porcine circovirus type 2 and Haemophilus parasuis (serotype 4 + serotype 5) vaccine composition (V1)

[0159] Inactivated PCV2 virus solution (containing 10 6.0 TCID 50 / ml) 45ml

[0160] Inactivated Haemophilus parasuis type 4 strain (containing 4.5×10 9.0 cfu / ml) 22.5ml

[0161] Inactivated Haemophilus parasuis type 5 strain (containing 4.5×10 9.0 cfu / ml) 22.5ml

[0162] Montanide TM Gel 10ml

[0163] (2) Porcine circovirus ty...

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Abstract

The invention relates to a polyvalent vaccine composition for porcine, and in particular relates to a vaccine composition capable of resisting infection of porcine circovirus type 2 (PCV 2) and haemophilus parasuis (HPS) at the same time. The vaccine composition comprises at least one PCV2 antigen, at least one HPS, as well as vector, an excipient and an adjuvant available in the field of veterinary medicine. The vaccine composition can be used for preventing and treating PCV2 related diseases and HPS diseases.

Description

Technical field [0001] The invention relates to a vaccine composition for pigs, in particular to a vaccine composition for simultaneously resisting porcine circovirus type 2 (PCV2) and Haemophilus parasuis (HPS) infection, and a preparation method and application thereof. Background technique [0002] Porcine circovirus (PCV) is the smallest animal virus found so far. It is now known that there are two serotypes of PCV, namely PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus, which is the main pathogen of multiple system failure syndrome (PMWS) in weaned piglets. The clinical features of PMWS include weight loss, pale skin, dyspnea, diarrhea, and jaundice. Pathological changes can be seen systemic lymph nodes swelling, especially inguinal lymph nodes swelling up to 5 to 10 times; lungs are mainly diffuse, interstitial pneumonia changes, the texture is hard as rubber, the surface is generally gray-brown mottled appearance; kidney There are many changes...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/20A61P31/04C12N1/20C12R1/21
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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