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Mink hemorrhagic pneumonia and botulism combined inactivate vaccine and preparing method thereof

A dual inactivated vaccine, hemorrhagic pneumonia technology, applied in vaccines, multivalent vaccines, veterinary vaccines, etc., can solve problems such as labor, increased cost, and impact on immune effects

Active Publication Date: 2016-07-13
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Individual immunization of pig mink hemorrhagic pneumonia bivalent inactivated vaccine and Clostridium botulinum poisoning (type C) inactivated vaccine has problems such as cumbersome, time-consuming, laborious, and increased costs, and it is easy to cause missed species, which directly affects the immune effect

Method used

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  • Mink hemorrhagic pneumonia and botulism combined inactivate vaccine and preparing method thereof
  • Mink hemorrhagic pneumonia and botulism combined inactivate vaccine and preparing method thereof
  • Mink hemorrhagic pneumonia and botulism combined inactivate vaccine and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] - Bacteria inspection

[0067] 1. Morphological and biochemical properties

[0068] Pseudomonas aeruginosa strain G type WD005, type B DL007 strain, and type C strain ZC118 were Gram-negative, short bacilli with obtuse round ends. The biochemical characteristics conform to the characteristics of this bacteria in bacterial taxonomy.

[0069] Clostridium botulinum type C strain C62-4 is a Gram-positive large bacillus, and old cultures often become Gram-negative, producing atopic spores. The biochemical characteristics should conform to the characteristics of the bacteria in bacterial taxonomy.

[0070] 2. Cultivate traits

[0071] Pseudomonas aeruginosa G-type WD005, B-type DL007, and C-type ZC118 grow on nutrient agar plates as round, smooth, moist, flat colonies; when grown on MacConkey medium, the colonies are off-white; On the sheep blood agar plate, it can produce obvious β-hemolysis; when it grows on the medium for the determination of pyocyanin (PDP), it will p...

Embodiment 3

[0097] - vaccine preparation

[0098] 1. Preparation of Pseudomonas aeruginosa bacterial solution

[0099] (1) Primary seed propagation and identification Pseudomonas aeruginosa G type WD005 strain, B type DL007 strain, and C type ZC118 strain were cultured respectively. Inoculate strains into improved Martin broth medium, culture at 37°C for 12-18 hours, streak inoculate nutrient agar plate containing 5% newborn bovine serum, culture at 37°C for 18-36 hours, pick 5-10 typical colonies, inoculate Several branches of sheep blood agar slant were cultured at 37°C for 18-36 hours as primary seeds. Sampling shall be pure with a nutrient agar plate containing 5% newborn bovine serum for pure inspection. Store at 2-8°C, and the service life should not exceed 14 days.

[0100] (2) Secondary seed propagation and identification Pseudomonas aeruginosa G type WD005 strain, B type DL007 strain, and C type ZC118 strain were cultured respectively. The primary seeds were inoculated into t...

Embodiment 4

[0122] - Vaccine testing

[0123] 1. Properties After standing still, the upper layer is a clear liquid, and the lower layer is a gray-white precipitate, which becomes a uniform suspension after shaking.

[0124] 2. The inspection of the filling capacity shall be carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and all of them are in compliance with the regulations.

[0125] 3. The sterility test was tested according to the appendix of the current "Chinese Veterinary Drug Code", and all of them grew aseptically.

[0126] 4. Choose one of the following methods for safety inspection.

[0127] (1) Three batches of laboratory products were tested with mink and injected subcutaneously into 2-month-old healthy susceptible mink (Pseudomonas aeruginosa type G, B, and C slide agglutination test antibodies were negative; Clostridium botulinum neutralizing antibodies were negative ) of 5 minks, 2.0ml of the vaccine was subcutaneously injected int...

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Abstract

The invention relates to a mink hemorrhagic pneumonia and botulism combined inactivate vaccine and a preparing method thereof.Strain WD005, strain DL007 and strain ZC118 of pseudomonas aeruginosa for vaccine manufacturing and detecting are obtained through clinical isolation, and the strains are higher in pertinence and more comprehensive in protection for current mink hemorrhagic pneumonia epidemic serotype; by means of virulence tests and immunogenicity tests, the immunogenicity is good; a strain C-type clostridium botulinum C62-4 has the advantage of being superior in immunogenicity.The combined inactivate vaccine can prevent attack of G-type pseudomonas aeruginosa, B-type pseudomonas aeruginosa, C-type pseudomonas aeruginosa and C-type clostridium botulinum to minks at the same time, and has the advantages that the number of inoculation times is reduced, and using is convenient.The labor intensity of immunization is relieved, the immune cost is reduced, the stress reaction of animals is reduced, and the vaccine is more economical and reliable.

Description

technical field [0001] The invention relates to a mink hemorrhagic pneumonia and Clostridium botulism dual inactivated vaccine and a preparation method thereof. It belongs to the field of veterinary biological products. Background technique [0002] Mink hemorrhagic pneumonia is an acute infectious disease of mink caused by Pseudomonas aeruginosa. The disease mainly occurs from August to October every year. It is characterized by hemorrhagic pneumonia and sepsis in mink. Mink hemorrhagic pneumonia was first reported by Knox et al. in Denmark in 1953, and later it was also reported in other European countries, North America, South America, and Asia. In my country, Pan Liansheng reported the disease for the first time, and there have been reports since then (Pan Liansheng. Preliminary report on the development of lipopolysaccharide vaccine of Pseudomonas mink. Acta Economic Zoology, 1984, (1): 1-3). After 2001, the disease broke out frequently in various provinces of my cou...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61K39/104A61K39/08A61P31/04
CPCA61K39/08A61K39/104A61K2039/521A61K2039/552A61K2039/70A61K2300/00
Inventor 宋晓飞张小军王蕾陈辉田真王景伟史须斌马珊珊
Owner QILU ANIMAL HEALTH PROD
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