Vaccine composition, preparation method and application thereof

A vaccine composition and the technology of the composition are applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, bacterial antigen components, etc., which can solve problems such as mixed infections and economic losses, and achieve avoidance of adverse reactions, reduction of vaccination times, immunity Convenient and fast effect

Active Publication Date: 2014-07-09
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this disease is an old disease, its importance has been highlighted in recent years due to frequent mixed infections with other pathogens such as PRRSV, circovirus, and swine fever virus, causing major economic losses.

Method used

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  • Vaccine composition, preparation method and application thereof
  • Vaccine composition, preparation method and application thereof
  • Vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 porcine circovirus type 2 antigen and mycoplasma hyopneumoniae antigen

[0031] 1 strain source

[0032] The type 2 strain of porcine circovirus used in the manufacture and inspection of this product is the PCV-2 strain SH strain, which was preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms. The preservation date: March 4, 2008, and the preservation number It is CGMCC No.2389.

[0033] The strain of Mycoplasma hyopneumoniae used in the manufacture and inspection of this product is HN0613, which was preserved in the China Center for Type Culture Collection (abbreviation: CCTCC; address: Wuhan University, No. 16, Luojiashan Road, Wuchang District, Wuhan City, Hubei Province), preservation date: 2012 June 13, 2012, deposit number: CCTCC NO.M2012230.

[0034] The classical swine fever virus strain used in the manufacture and inspection of this product is the attenuated strain of classic...

Embodiment 2

[0055] The preparation of embodiment 2 swine fever, porcine circular ring and mycoplasma hyopneumoniae triple mixed vaccine composition

[0056] 1 Preparation of porcine circular and mycoplasma hyopneumoniae dual inactivated vaccine

[0057] 1.1 Preparation of preservatives

[0058] 1% (w / v) thimerosal aqueous solution: 1g of thimerosal is dissolved in 100ml of purified water, and autoclaved at 121°C for 30 minutes for later use.

[0059] 1.2 Preparation of diluent

[0060] Sterile PBS buffer solution: Dissolve 8g sodium chloride, 0.25g potassium chloride, 3.63g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate in 900ml purified water, then dilute to 1L, autoclave at 121°C for 30min spare.

[0061] 1.3 Vaccine adjuvant treatment

[0062] Sterilization of Gel01 (SEPPIC, France) adjuvant: transfer Gel01 (SEPPIC, France) adjuvant into a sterilizable container, and autoclave at 121°C for 30 minutes for later use.

[0063] 1.4 Matching seedlings

[0064] Thr...

Embodiment 3

[0072] The swine fever of embodiment 3 different antigen contents, porcine circular ring and mycoplasma hyopneumoniae triple combination vaccine effectiveness test

[0073] 1 test material

[0074] Vaccine 1 (mycoplasma hyopneumoniae 10) prepared in embodiment 2 7 MHDCE / Toufen, porcine circovirus type 2 10 4.0 TCID 50 / head), vaccine 2 (Mycoplasma hyopneumoniae 10 8 MHDCE / Toufen, porcine circovirus type 2 10 5.0 TCID 50 / head), vaccine 3 (Mycoplasma hyopneumoniae 10 9 MHDCE / Toufen, porcine circovirus type 2 10 6.0 TCID 50 / head), vaccine 4 (Mycoplasma hyopneumoniae 10 10 MHDCE / Toufen, porcine circovirus type 2 2×10 6.0 TCID 50 per serving) and vaccine 5 (Mycoplasma hyopneumoniae 2×10 10 MHDCE / Toufen, porcine circovirus type 2 2×10 6.0 TCID 50 / toufen).

[0075] 3 to 4 weeks old, weaned piglets without antibodies to CSF, Circular swine and Mycoplasma hyopneumoniae.

[0076] 2 test method

[0077] 2.1 Safety test

[0078] Select 30 weaned piglets aged 3 to ...

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Abstract

The invention relates to a vaccine composition, which contains classical swine fever virus antigen, porcine circovirus type 2 antigen, mycoplasma hyopneumoniae antigen and a vaccine adjuvant. The invention also provides a method for preparing the vaccine composition, a water-soluble vaccine adjuvant is used for preparing a mixture of the porcine circovirus type 2 antigen and mycoplasma hyopneumoniae antigen, and the mixture is taken as a diluent for diluting classical swine fever virus antigen. The vaccine composition has the advantages of simple preparation method, high titer content of the vaccine, and convenient and fast immunization, one time immunization can simultaneously preventing or treating swine fever, porcine circovirus type 2 antigen and mycoplasma hyopneumoniae, immunization cost is reduced, the immunization program is saved, and the application is more economic and reliable.

Description

technical field [0001] The invention relates to a combined vaccine composition, in particular to a triple mixed vaccine composition of swine fever, porcine circular ring and mycoplasma hyopneumoniae and its preparation method and application. Background technique [0002] Classical swine fever is an acute, febrile, highly contagious infectious disease of pigs, characterized by high temperature and microvascular degeneration, which can cause systemic hemorrhage, necrosis, and infarction. In the early years, it was also known as hog cholera, which was extremely harmful to pigs and caused great losses to the pig industry. However, due to the mixed infection and the influence of some immunosuppressive pathogens, the control effect of swine fever vaccine alone was reduced, and there were always sporadic outbreaks of swine fever. . [0003] Porcine circovirus type 2 (Porcine circovirus, abbreviation: PCV) is the smallest animal virus found so far. PCV is known to have two seroty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/14A61P31/20A61K39/187A61K39/12A61K39/02
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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