31 results about "Classical swine fever virus antigen" patented technology

The invention discloses a swine fever vaccine heat-resistant freeze-drying protective agent, and a preparation method and an application thereof. The heat-resistant freeze-drying protective agent comprises the following components: trehalose, polyvinylpyrrolidone (PVP), gelatin, sodium glutamate, D-sorbic alcohol, polypeptone, L-arginine, vitamin C and water for injection and is prepared by dissolving and degerming. The heat-resistant freeze-drying protective agent is mixed with swine fever virus antigen liquid according to the volume ratio of 1:(08-1.2) and is frozen and dried after being packaged to obtain swine fever vaccine. The prepared heat-resistant freeze-drying protective agent is simple in formula, is easy to prepare, is suitable for large-scale production and has good protection efficacy on the vaccine. The prepared vaccine has the characteristics of safety and sterility, stable quality, heat resistance and long saving time. The swine fever vaccine heat-resistant freeze-drying protective agent solves the problems that the vaccine is required to be frozen at a low temperature, is not convenient to store and the like in the transportation process. Through the swine fever vaccine heat-resistant freeze-drying protective agent, the swine fever vaccine can be saved for above 30 months at a temperature of 2-8 DEG C.

The invention belongs to the field of animal immune detection, and in particular relates to a test paper card for quantitative detection of swine fever virus antibody with double antigen sandwiches and double detection lines. The test card includes sample pad, classical swine fever virus E2 protein gold standard pad, rabbit IgG gold standard pad, absorbent pad, double detection line coated with classical swine fever virus E2a protein, and nitrocellulose membrane of goat anti-rabbit IgG quality control line and PVC rubber sheet; colloidal gold adopts double-antigen sandwich and double detection line; the classical swine fever virus gold standard pad is coated with the protein antigen secreted by Escherichia coli BL21 / pE2 with the preservation number CCTCC NO: M2018060. E2 antigen and gold-labeled rabbit IgG; the nitrocellulose membrane is coated with the CSFV E2a antigen constructed from the protein antigen secreted by Escherichia coli BL21 / pE2a with the preservation number CCTCC NO: M2018059, constituting a double detection line area and The quality control line region is composed of goat anti-rabbit IgG.

The invention relates to a swine fever cell-adapted virus, a live swine fever virus vaccine prepared therefrom and a preparation method thereof. In the method, the adaptive virus of classical swine fever virus is used as seedling virus seed, and the original virus seed, basic virus seed and production virus seed of classical classical virus are prepared by successive cell culture, and then the virus seed is produced by the adaptive virus of classical swine fever virus in the passaged cell. After multiplication, the qualified virus liquid is continuously harvested as the antigen for the attenuated swine fever vaccine, and the swine fever cell-adapted live vaccine is obtained through seedling preparation, subpackaging, and freeze-drying. The preparation of poison seeds solves animal welfare issues such as slaughtering rabbits for the prevention of swine fever, and reduces the possible risk of exogenous factors and potential shedding of poison in the preparation of spleen poison. The method for preparing a live vaccine of swine fever virus has the advantages of short virus propagation time, many harvest times, large amount of virus liquid harvested per unit cell, high virus content and low production cost. The prepared swine fever virus live vaccine has good safety and high immune efficacy.

The invention relates to a vaccine composition, which contains classical swine fever virus antigen, porcine circovirus type 2 antigen, mycoplasma hyopneumoniae antigen and a vaccine adjuvant. The invention also provides a method for preparing the vaccine composition, a water-soluble vaccine adjuvant is used for preparing a mixture of the porcine circovirus type 2 antigen and mycoplasma hyopneumoniae antigen, and the mixture is taken as a diluent for diluting classical swine fever virus antigen. The vaccine composition has the advantages of simple preparation method, high titer content of the vaccine, and convenient and fast immunization, one time immunization can simultaneously preventing or treating swine fever, porcine circovirus type 2 antigen and mycoplasma hyopneumoniae, immunization cost is reduced, the immunization program is saved, and the application is more economic and reliable.

The present invention disclosed a dual -peak camel source anti -swine fever rabbing weak toxic vaccine plant E2 antigen heavy chain antibody VHH and use to solve the existing existing swine fever virus antigen diagnostic reagent research and development process.The production and preparation of swine fever virus antibodies.A twin -peak camel source anti -swine fever rabbit, weak poisonous vaccine, E2 antigen heavy chain antibody VHH, the antibody has nucleotide sequence: SEQ ID No.1, SEQ ID No.2 or SEQ ID No.3.The Shuangfeng Camel anti -swine fever rabbit bunocide vaccine plant E2 antigen heavy chain antibody VHH is used in the application of the application of swine fever virus antigen diagnostic reagents.The invention of the present invention is characterized by the first time obtaining the use of Shuangfeng camel screening to obtain the VHH antibody coding sequence of the VHH antibody coding of the antigen E2 of the anti -poison vaccine plant of the swine fever virus.Dopoline antibody has the same antigen -binding capacity, which can replace swine fever virus Dopolon antibodies in the study of swine fever virus antigen diagnostic reagents.

The invention provides a vaccine composition, which contains an immunological amount of classical swine fever virus antigen, an immune amount of Haemophilus parasuis antigen, an immune amount of porcine circovirus type 2 antigen and veterinary acceptable carrier. The vaccine composition can effectively prevent and treat swine fever, porcine circovirus disease and Haemophilus parasuis disease at the same time, and its single injection can achieve the immune effect of single vaccine injection twice, with small side effects, long immunization period, and time-consuming Less, less effort.

A recombinant vector for transforming a plant, a plant transformed with the recombinant vector, a plant-made classical swine fever virus antigen pmE2 protein expressed in the plant and uses thereof is provided. By using a recombinant vector having a polynucleotide encoding a GP55 protein of CSFV according to the present invention; and a polynucleotide encoding a cellulose-binding domain protein; and a plant transformed with the recombinant vector, a plant-made classical swine fever virus antigen pmE2 protein may be produced with high efficiency, and has higher safety and stability than those achieved by other production methods. Also, since the plant-derived classical swine fever virus antigen protein pmE2 has a cellulose-binding domain (CBD) protein, it may be usefully used as an effective marker to determine a virus exposure pathway and an antibody producing pathway.

The invention relates to a bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof. According to the method, a porcine circovirus 2 subunit antigen and a swine fever virus antigen are mixed and lyophilized to prepare the bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection; or a porcine circovirus 2 totivirus antigen inactivated vaccine is used as a diluent to dilute a swine fever virus vaccine, so as to prepare a bivalent vaccine composition kit for resisting swine fever virus and porcine circovirus 2 infection. The bivalent vaccine composition does not produce immunity interference; after immunization, the swine fever virus antigen speeds up in immunoreaction; the protective antibodies provided by the vaccine composition reach an immunization protective dose one week earlier than a single vaccine antibody; and the antibodies produced by the porcine circovirus vaccine in the bivalent vaccine have similar effects as that produced by single vaccine immune. The method has the advantages of simple vaccine production process and high production efficiency. The animal experiments prove that the effect of the vaccine composition is better that that of single vaccine immune, therefore the invention has great application value.

The invention provides a vaccine composition, which comprises: immunizing amount of classical swine fever virus antigen, immunizing amount of porcine pseudorabies virus antigen and immunizing amount of Haemophilus parasuis antigen. The vaccine composition is safe to use, has controllable quality, good immune effect, and the protection rate of the virus challenge reaches 80-100%. Satisfactory immune effect can be achieved by intranasally immunizing 0-3 day-old piglets and intramuscularly immunizing 50-day-old piglets.

The present invention provides an African swine fever virus chimeric protein, the chimeric protein comprises: (1) African swine fever virus p72 domain I; (2) African swine fever virus p72 domain II; (3) African swine fever virus viral p72 domain III; and (4) African swine fever virus antigenic protein. The chimeric protein provided by the present invention uses the African swine fever virus p72 protein as the backbone for the first time, and better displays the antigenic epitopes of the African swine fever virus antigenic proteins p54, p30, CD2v and p12, has good immune effect, and can produce significant Humoral and cellular immunity.

The invention provides a vaccine composition. The vaccine composition comprises hog cholera virus antigen and porcine reproductive and respiratory syndrome (PRRS) antigen, wherein the hog cholera virus antigen is a hog cholera rabbit attenuated strain, the PRRS antigen is a high-pathogenicity PRRS virus, and the content ratio of the PRRS antigen and the hog cholera virus antigen is 100:1-100:10. The vaccine composition can eliminate the interference between the two types of antigen, achieves the purpose of preventing two infectious diseases by one injection, and greatly reduces immunization times and animal stress response.