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Swine fever virus antibody detection kit

A swine fever virus and antibody detection technology, which is used in biological testing, measuring devices, material inspection products, etc. Reliable test results

Inactive Publication Date: 2019-12-10
QINGDAO HARRENS INSPECTION & TESTING INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection sensitivity of the HA method is low, the sample needs to be inactivated, and the error of naked eye interpretation is large; the detection sensitivity and specificity of RT-PCR and ELISA are high, but expensive equipment is required, the detection time is long, and professional and technical personnel are required to operate; colloidal gold immunochromatography The operation is simple, the detection time is short, but the accuracy is not high, the detection range is narrow, and it cannot be accurately quantified

Method used

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  • Swine fever virus antibody detection kit
  • Swine fever virus antibody detection kit
  • Swine fever virus antibody detection kit

Examples

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Embodiment 1

[0044]A swine fever virus antibody detection kit, comprising reagent R1 and reagent R2, said reagent R1 is a buffer solution of pH 6.0-8.0, which includes stabilizer, polymer accelerator, surfactant and preservative; said reagent R2 is a latex reagent of classical swine fever virus antigen, which includes a stabilizer, latex particles of classical swine fever virus E2 antigen, a preservative and a buffer.

[0045] 1. Preparation of Latex Microspheres

[0046] Using nano-gold as latex microspheres, the preparation method is as follows: add 100mL of ultrapure water to a cleaned 1000mL round bottom flask, put 1 magnetic stirrer, add 1mL of 1% chloroauric acid solution, stir and heat at 600rpm When the solution boils, quickly add 1.5 mL of 1% trisodium citrate solution, continue heating and stirring for 10 minutes, the color of the solution turns black first and then red, stop heating and continue stirring for 10 minutes, then stop stirring and return to room temperature and use u...

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PUM

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Abstract

The invention discloses a swine fever virus antibody detection kit, and belongs to the technical field of biological detection. The swine fever virus antibody detection kit comprises a reagent R1 anda reagent R2, wherein the reagent R1 is a buffer solution with the pH value of 6.0-8.0 and comprises a stabilizer, a polymer accelerator, a surfactant and a preservative; the reagent R2 is a swine fever virus antigen latex reagent and comprises a stabilizer, swine fever virus E2 antigen latex particles, a preservative and a buffer solution. The kit disclosed by the invention has the advantages ofhigher detection sensitivity and specificity, good stability, simplicity in operation and short reaction time, is suitable for a full-automatic biochemical analyzer, a special protein instrument and aspectrophotometer, does not generate precipitate after reaction, and facilitates cleaning of a reaction vessel.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a swine fever virus antibody detection kit. Background technique [0002] Classical swine fever (CSF) is an acute, febrile, contagious infectious disease caused by classical swine fever virus (CSFV). The organization listed it as a class A infectious disease. The disease spreads fast, is popular, has a high morbidity rate and a high lethality rate, and is extremely harmful to my country's pig industry. The clinical manifestations of swine fever are mostly subacute, chronic or atypical, and occasionally acute, which can occur all year round, but occurs frequently in spring, autumn, and winter, and may occur in pigs of different ages, sexes, and breeds. Only by mastering clinical diagnosis and scientific detection methods, and accurately monitoring the immune antibody level and the law of antibody growth and decline in pig herds, can the epidemic of swine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/544G01N33/543G01N33/531
CPCG01N33/531G01N33/54346G01N33/544G01N33/56983G01N33/6854G01N2333/183
Inventor 刘刚梁德勇刘欢付建瑞张鹏飞宋彩玲
Owner QINGDAO HARRENS INSPECTION & TESTING INC
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