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Vaccine composition, and preparation method and application thereof

A vaccine composition and antigen technology, which is applied in the field of vaccine compositions, can solve the problems of triple combination vaccine, increased intensity, stress response of pig herds, etc., for which there is no Haemophilus parasuis for swine fever, so as to reduce costs and inoculations. The number of times, the effect of boosting the immune effect

Active Publication Date: 2015-01-21
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of the three vaccines requires secondary immunization, which increases the intensity of the work, and multiple immunizations are also likely to cause stress reactions in pigs
[0006] There is currently no triple combination vaccine for classical swine fever, porcine circovirus, and Haemophilus parasuis

Method used

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  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of swine fever antigen, porcine circovirus antigen and Haemophilus parasuis antigen

[0040] 1 Preparation of swine fever antigen

[0041] 1.1 Use ST cells (purchased from ATCC) that are highly sensitive to classical swine fever virus (swine fever lapinized attenuated strains are preserved by the China Veterinary Drug Supervision Institute, with the preservation number AV1412) containing 0.125% pancreatin and 0.03% The digestion solution of EDTA is digested and dispersed. After the cell count, the cell culture flask is inoculated, and 1.5%-5% FBS MEM cell culture solution is added. At the same time, the seed poison is added according to the MOI=0.01-0.6 ingestion dose. The more preferred dose is MOI=0.1-0.6, and it is more preferable that the poisoning dose is MOI=0.2-0.4, and it is cultured in a 34-37°C incubator, and further preferably the culture temperature is 34-35°C.

[0042] After three days of culture, the first collection of poisons is carried o...

Embodiment 2

[0059] Example 2 Preparation of a triple mixed vaccine composition of swine fever, swine ring and Haemophilus parasuis

[0060] Preparation of 1 inactivated vaccine against Haemophilus suis and porcine ring

[0061] 1.1 Preparation of preservatives

[0062] 1% (w / v) thimerosal aqueous solution: 1g thimerosal dissolved in 100ml purified water, autoclaved at 121°C for 30 minutes for later use.

[0063] 1.2 Preparation of diluent

[0064] Sterile PBS buffer solution: Dissolve 8g sodium chloride, 0.25g potassium chloride, 3.63g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate in 900ml purified water, then dilute to 1L, autoclave at 121°C for 30min spare.

[0065] 1.3 Vaccine adjuvant treatment

[0066] Gel01 adjuvant sterilization: transfer Gel01 adjuvant into a sterilizable container, autoclave at 121°C for 30 minutes for later use.

[0067] 1.4 Matching seedlings

[0068] After aseptic operation, the concentrated Haemophilus parasuis antigen, porcine circovirus type 2 antige...

Embodiment 3

[0076] Example 3 Efficacy test of the triple mixed vaccine of swine fever, pig circle and Haemophilus parasuis with different antigen content

[0077] 1 Test materials

[0078] Vaccine 1 prepared in Example 2 (Haemophilus parasuis 10 7 CFU / tou, swine fever virus 10 4.0 TCID 50 / Toufen, Porcine Circovirus 10 4.0 TCID 50 / Head), vaccine 2 (Haemophilus parasuis 10 8 CFU / tou, swine fever virus 10 5.0 TCID 50 / Toufen, Porcine Circovirus 10 5.0 TCID 50 / Head), vaccine 3 (Haemophilus parasuis 10 9 CFU / tou, swine fever virus 10 6.0 TCID 50 / Toufen, Porcine Circovirus 10 6.0 TCID 50 / Head), vaccine 4 (Haemophilus parasuis 10 10 CFU / tou, swine fever virus 10 6.0 TCID 50 / Toufen, Porcine Circovirus 10 6.0 TCID 50 / Head) and vaccine 5 (Haemophilus parasuis 2×10 10 CFU / tou, swine fever virus 10 6.0 TCID 50 / Toufen, Porcine Circovirus 10 6.0 TCID 50 / Toufen).

[0079] Weaned piglets at the age of 3-4 weeks without swine fever, pig ring and Haemophilus parasuis antibodies.

[0080] 2 Test metho...

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Abstract

The invention provides a vaccine composition. The vaccine composition contains an immune amount of classical swine fever virus antigens, an immune amount of Haemophilus parasuis antigens, an immune amount of type 2 porcine circovirus antigens and a veterinarily acceptable carrier. The vaccine composition can effectively prevent and treat swine fever, the porcine circovirus disease and the Haemophilus parasuis disease, can reach double single vaccine injection immunization effect through one time injection, and has the advantages of few side reactions, long immune period, short time and less labor consumption.

Description

Technical field [0001] The invention relates to a vaccine composition. Background technique [0002] Swine fever is an acute, febrile, and highly contact infectious disease of pigs. The main feature is high temperature and microvascular degeneration that cause systemic bleeding, necrosis, and infarction. In the early years, it was also called swine cholera, which was extremely harmful to pigs and caused heavy losses to the pig industry. [0003] Porcine circovirus (Porcine circovirus, abbreviation: PCV) is the smallest animal virus found so far. It is now known that there are two serotypes of PCV, namely PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus. It is the main pathogen of Postweaning Multisystemic Wasting Syndrome (PMWS) in weaned piglets. It was first discovered in Canada (1991) and soon occurred and spread in some countries in Europe, America and Asia, including my country. In addition to PMWS, PDNS (porcine dermatitis and nephrotic syndrome), ...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/04A61P31/14A61P31/20
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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