Method for detecting pig plague virus specific antibody and its ELISA reagent kit

An enzyme-linked immunoassay reagent and swine fever virus technology, which is applied in the direction of measuring devices, material analysis and instruments through observation of the impact on chemical indicators, can solve the problem of affecting the effect of attenuated live vaccination, the vaccine is invalid, and cannot produce Virus immunity and other issues
CN101144818AInactive Publication Date: 2008-03-19TSINGHUA UNIV +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2008-03-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting the specific antibody of classical swine fever virus and a special ELISA kit thereof. The kit includes a classical swine fever virus antigen and an enzyme-labeled classical swine fever virus single-epitope specific antibody; the said swine fever virus antigen is a polypeptide containing one or more than one amino acid residue sequence described in sequence 1. The detection reagent of the classical swine fever virus specific antibody of the present invention can carry out effective detection to the classical swine fever virus specific antibody by solid-phase antigen competition ELISA (blocking method); The B cell epitope ensures the differential diagnosis, and the high degree of conservation of the epitope among various strains ensures the specificity of detection.
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Description

technical field

[0001] The invention relates to a method for detecting the specific antibody of classical swine fever virus and a special ELISA reagent kit thereof. Background technique

[0002] Swine fever is a highly contagious, highly pathogenic and highly lethal severe animal disease caused by swine fever virus, which is extremely harmful to the pig industry and is one of the legally reported severe infectious diseases determined by the World Organization for Animal Health , is also one of the first class of animal diseases identified in my country. For a long time, the measures taken by our country for the swine fever epidemic are "prevention and control". Prevention means vaccination with live attenuated swine fever vaccine (rabbitized attenuated swine fever vaccine) through immunization; control means controlling the epidemic, monitoring, isolation, and even partial "culling". In the prevention and control of swine fever, it is very important to monitor and detect th...

Claims

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