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Chog cholera and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof

A technology of swine pseudorabies and dual vaccine, which is applied in the field of swine vaccine, can solve the problems of swine fever infection and the difficulty of swine fever vaccine to play an epidemic prevention role, and achieve the effect of improving protection efficiency and speed.

Pending Publication Date: 2022-04-05
成都博宠生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been studies on swine fever and porcine pseudorabies dual vaccine, but they have ignored the interference phenomenon of pseudorabies virus on swine fever antibodies in the process of immunization practice. This interference phenomenon makes simultaneous immunization of swine fever vaccine The overall level of the animal's swine fever antibody is very low, which makes it difficult for the swine fever vaccine to prevent the epidemic for a period of time, resulting in the infection of swine fever

Method used

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  • Chog cholera and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof
  • Chog cholera and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof
  • Chog cholera and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof

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Experimental program
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Embodiment 1

[0075] vaccine preparation

[0076] (1) Preparation of target gene

[0077] Design primer, amplify classical swine fever virus and porcine pseudorabies virus antigenic protein gene by the method for PCR; The amplification primer of the antigenic protein gene designed in this process one is:

[0078] CFSV E2 upstream primer: 5'-TAGGCTTCTATTCTAGTCTGCGCAATA-3' (contains XbaI restriction site)

[0079] CFSV E2 downstream primer: 5'-GAATGAAGATTATGCTATGGTACGTAG-3' (contains KpnI restriction site)

[0080] PRV gB upstream primer is: 5'-CCAGTCGCAGGCCACCGTGAGTG-3' (contains KpnI restriction site)

[0081] The downstream primer of PRV gB is: 5'-ATCGCGTGCTCTTCATGGAGATC-3' (XbaI restriction site)

[0082] Among them, the amplified length of CSFV E2 gene is 294bp, and the amplified length of PRV gB gene is 632bp.

[0083] (2) Construction of recombinant replication-deficient chimpanzee adenovirus shuttle vector

[0084] Insert the antigenic protein antigenic protein gene of the classi...

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Abstract

The invention discloses a swine fever and swine pseudorabies bivalent vaccine, which is prepared by inserting a swine pseudorabies virus gB antigen and a swine fever virus E2 antigen into a replication-defective chimpanzee adenovirus shuttle vector, the gene sequence of the swine pseudorabies virus gB antigen is SEQ ID No.1, and the gene sequence of the swine fever virus E2 antigen is as shown in SEQ ID No.2. The preparation method comprises the following steps: 1) preparing a classical swine fever virus antigen protein E2 gene and a porcine pseudorabies virus antigen protein gB gene; (2) constructing a recombinant replication-deficient chimpanzee adenovirus shuttle vector; (3) obtaining replication-defective chimpanzee adenovirus plasmids; and (4) obtaining the recombinant adenovirus with a uniform genome structure, and preparing the recombinant adenovirus into the vaccine with the required dosage form as required. According to the invention, the immunosuppression phenomenon occurring when the porcine pseudorabies virus and the hog cholera virus are simultaneously used as antigens for immunization can be avoided, and an unexpected effect that an immune receptor can generate a hog cholera antibody within 7 days of immunization is obtained.

Description

technical field [0001] The invention relates to the field of porcine vaccines, in particular to a dual vaccine of swine fever and porcine pseudorabies and its preparation method and application. Background technique [0002] Classical swine fever (CSFV) is an acute, febrile, fatal disease caused by classical swine fever virus (Classical swine feve Virus CSFV), which has caused great losses to the swine industry in the world. E2 protein is the main protective antigen of classical swine fever virus, which can induce the body to produce high levels of specific neutralizing antibodies, and protect pigs against the lethal CSFV attack, and plays an important role in the development of new vaccines. [0003] Pseudorabies virus belongs to the family Herpesviridae and the genus Porcine Herpesvirus. Virus particles are round, with a diameter of 150-180 mm and a nucleocapsid diameter of 105-110 mm. The outermost layer of the virion is the viral envelope, which is a lipid bilayer struc...

Claims

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Application Information

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IPC IPC(8): A61K39/245A61K39/12A61P31/22A61P31/14C12N15/861C12R1/93
Inventor 杨鑫刘鑫李子鸣李伊
Owner 成都博宠生物科技有限公司
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