Vaccine composition, preparation method and application thereof

A vaccine composition and antigen technology, applied in the direction of non-active ingredients of polymer compounds, pharmaceutical formulas, medical preparations of non-active ingredients, etc., can solve the problems of maternal antibody interference, do not reduce the immune effect, etc., and achieve quality control , good immune effect

Active Publication Date: 2015-04-15
PU LIKE BIO ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The inventors have found that if nasal drop immunization is carried out with the vaccine composition of swine fever, porcine pseudorabies and Haemophilus parasuis, the problem of maternal antibody interference can be solved, and a good effect on swine fever, porcine pseudorabies and Haemophilus parasuis can be produced. immune protection; when applied, live vaccines for swine fever, porcine pseudorabies and inactivated Haemophilus parasuis vaccines can be used in the form of diluted live vaccines with inactivated vaccines, and can also be mixed in a certain proportion and then added with a lyoprotectant for freeze-drying. , easy to transport and does not reduce the immune effect; in addition, it was also found that the inactivated Haemophilus parasuis vaccine has an immune-enhancing effect on swine fever and porcine pseudorabies vaccine, and the latter antigen can be halved to achieve the same effect as a single vaccine

Method used

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  • Vaccine composition, preparation method and application thereof
  • Vaccine composition, preparation method and application thereof
  • Vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Preparation of Anti-Swine Fever Virus, Porcine Pseudorabies Virus and Haemophilus parasuis Vaccine Composition

[0047] 1. Preparation and testing of CSFV antigen solution

[0048] (1) The ST cells (purchased from ATCC) (American type culture collection, American type culture collection) grown into a good monolayer highly sensitive to classical swine fever virus were treated with 0.125wt% trypsin and 0.03wt% EDTA ( ethylenediaminetetraacetic acid) for digestion and dispersion, cell counting, and after inoculation at a suitable density, MEM containing 3wt%-5wt% FBS (fetal bovine serum) (purchased from PAA Company, batch number A15110-1462) was added (purchased from GIBCO, batch number 856833), and at the same time add seed virus according to M.O.I. (multiplicity of infection) = 0.1-0.2 inoculated dose, and place in an incubator at 34-37°C for cultivation.

[0049] (2) After three days of cultivation, the first poisoning was carried out. After the poisoning,...

Embodiment 2

[0069] Example 2 Correlative inspection of classical swine fever virus, porcine pseudorabies virus and Haemophilus parasuis vaccine

[0070] 1. Physical property test, sterility test

[0071] Vaccines 1 to 8 passed the physical property test and sterility test, and the detailed results are shown in Table 3:

[0072] Table 3 Vaccine physical properties test and sterility test

[0073]

[0074]

[0075] Note: T.G means thioglycolate medium, G.A means casease agar medium, G.P glucose-peptone medium; "-" means sterile growth.

[0076] 2 safety test

[0077] With 45 piglets negative for classical swine fever virus, porcine pseudorabies virus and Haemophilus parasuis antigen and antibody at the age of 2 to 3 weeks, 5 pigs in groups 1 to 3 were inoculated with 2 parts of inactivated vaccine partially diluted in each muscle 1 part of the sample to be tested from the live vaccine; 2 parts of freeze-dried diluted vaccine in each of the 5 heads of the 4th to 6th groups; the 5th ...

Embodiment 3

[0095] Embodiment 3 Application of swine fever, porcine pseudorabies and Haemophilus parasuis vaccine composition of the present invention

[0096] Trial 1: Comparative trial of vaccines

[0097] 1. Test materials The swine fever ELISA antibody detection kit was purchased from Beijing IDEXX Biotechnology Co., Ltd.; the porcine pseudorabies ELISA gB antibody detection kit was purchased from Beijing IDEXX Biotechnology Co., Ltd.

[0098] The vaccine batch number of the swine fever, porcine pseudorabies and Haemophilus parasuis vaccine of the present invention is several bottles of 20130202; the batch number of the live vaccine of swine fever produced by Guangdong Yongshun is 2012043, and the Haemophilus parasuis disease produced by Boehringer Ingelheim The batch number of live vaccine (z-1517) is 746-380, and the batch number of porcine pseudorabies live vaccine produced by Boehringer Ingelheim is 195A-23B.

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Abstract

The invention provides a vaccine composition including a hog cholera virus antigen in an immunizing dose, a porcine pseudorabies virus antigen in an immunizing dose, and a haemophilus parasuis antigen in an immunizing dose. The vaccine composition is safe to use, is controllable in quality and is excellent in an immune effect. The vaccine composition can achieve a challenge protective rate being 80-100%. The vaccine composition, when being used for immunizing piglets in 0-3 day ages in a nasal-dropping manner and for immunizing piglets in 50 day ages in a muscle injection manner, can achieve a satisfying immune effect.

Description

technical field [0001] The invention relates to a vaccine composition and its preparation method and application. Background technique [0002] Classical swine fever is an acute, febrile, highly contagious infectious disease of pigs, characterized by high temperature and microvascular degeneration, which can cause systemic hemorrhage, necrosis, and infarction. In the early years, it was also known as hog cholera, which was extremely harmful to pigs and caused heavy losses to the pig industry. [0003] Haemophilus parasuis disease is multiple serositis and arthritis in pigs caused by Haemophilus parasuis (Hps), also known as Glasser's Disease. Haemophilus parasuis can affect young pigs from 2 weeks to 4 months, mainly in post-weaning and nursery stages, usually seen in pigs of 5 to 8 weeks, the incidence rate can reach 40%, and the mortality rate can reach 50% %. The main clinical symptoms were cough, dyspnea, emaciation, lameness, and rough coat; the main necropsy lesions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K47/42A61K47/32A61P31/14A61P31/22A61P31/04G01N33/569A61K39/245A61K39/187A61K39/102
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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