Bactrian camel source C-strain E2 VHH and application

A technology of attenuated vaccine and heavy chain antibody, applied in the field of vaccines, can solve the problems of inability to obtain high-purity, high-affinity anti-swine fever virus antibody production and preparation, etc.

Active Publication Date: 2013-12-25
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a Bactrian camel-derived anti-swine fever rabinized attenuated vaccine strain E2 antigen heavy chain antibody VHH, to solve the existing problems in the research and development of the existing swine fever virus antigen diagnostic reagents that cannot obtain high-purity, high-affinity Problems in the production and preparation of antibodies against classical swine fever virus

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  • Bactrian camel source C-strain E2 VHH and application
  • Bactrian camel source C-strain E2 VHH and application
  • Bactrian camel source C-strain E2 VHH and application

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Embodiment 1

[0018] 1. Bactrian camel immunization, peripheral blood lymphocyte isolation and cDNA synthesis

[0019] 1. Materials and Methods

[0020] 1.1 Materials - test animals, antigens, adjuvants

[0021] Select two-hump female young Bactrian camels aged about 1 year old as immune recipient animals (these Bactrian camels are raised in the Gobi Desert near Jinchang City, Gansu Province all year round, mainly for sale by shearing camel hair. Animal owners only use winter grass Supplementary forage, water and salt were recalled during dry season, extreme drought in summer and lambing of female camels. They have never been vaccinated and are suitable recipient animals for the preparation of VHH antibodies.), the adjuvant uses 206 adjuvant, and the antigen is E2- GST antigen.

[0022] The E2 antigen of the CSFV vaccine strain (CSFV) was prepared using the existing method: the soluble E2 fusion protein (GST-tag) was purified by affinity chromatography as the antigen for immunizing Bactri...

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Abstract

The invention discloses bactrian camel source C-strain E2 VHH and application. The bactrian camel source C-strain E2 VHH and the application aim at solving the problem that production and preparation of high-purity high-appetency classical swine fever virus antibodies can not be obtained in the existing classical swine fever virus antigen diagnostic reagent research and development process. The bactrian camel source C-strain E2 VHH are characterized by being provided with a nucleotide sequence: SEQIDNO.1, SEQIDNO.2 or SEQIDNO.3. The invention further provides the application of the bactrian camel source C-strain E2 VHH in preparing a classical swine fever virus antigen testing diagnostic reagent. The bactrian camel source C-strain E2 VHH and the application have the advantages that the VHH coded sequence of C-strain antigen protein E2 is obtained for the first time through a Bactrian camel, ELISA experimental results show that the recombination VHH expressed in E.coli have the same antigen binding capacity as classical swine fever virus polyclone antiserum, and therefore the recombination VHH can replace the classical swine fever virus polyclone antiserum to be used for researching the classical swine fever virus antigen diagnostic reagent.

Description

technical field [0001] The invention belongs to the field of vaccines, and in particular relates to a Bactrian camel-derived heavy-chain antibody VHH against the E2 antigen of a attenuated vaccine strain of hog fever lanovirus. Background technique [0002] Classical swine fever (CSF), also known as swine fever or classical swine fever (CSF), is caused by CSF virus (CSFV). Classical swine fever is one of the important infectious diseases of pigs. Its pathological feature is degeneration of small blood vessel walls, leading to multiple hemorrhage, necrosis and infarction of internal organs. my country is a big pig raising country, and the most effective way to prevent and control swine fever is to prevent swine fever rabbit-type attenuated vaccine (C-strain). However, swine fever is still one of the most serious infectious diseases in the swine industry. In my country Preventing and controlling the occurrence of swine fever has important economic and social significance. ...

Claims

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Application Information

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IPC IPC(8): C07K16/10C12N15/13C12N15/63C12N1/21G01N33/569
Inventor 刘湘涛尹双辉尚佑军杨顺利田宏吴锦艳张克山刘永杰
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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