31results about How to "Real-time quantitative detection" patented technology

The invention relates to a heat cycle control polyase chain reaction biological detection system which is formed by a heat cycle heating freezing component (1), an optical system (2), an electron-multiplier phototube (3), a moving table (4), a step motor (5), a heat cycle temperature controller (6), a fluorescence detecting controller (7), a step motor driver (8) and a PC machine (9). It adopts fluorescence real-time detecting mode to analyze the augmentation of PCR form; it adopts thermoelectric semiconductor cryogenic technique to achieve PCR course and uses high-fidelity photoelectric system to detect the fluorescence signal; it adopts micro machine as upper machine to do heat cycle craft controlling and adopts light intensity to real-time detect the PCR biology detecting system.

The invention provides a high-integration equidistance equipartition nucleic acid amplification micro-fluidic chip. The high-integration equidistance equipartition nucleic acid amplification micro-fluidic chip sequentially consists of a cover glass layer, a reaction layer, a decorative layer and a sealing layer from bottom to top in a thermal bonding manner, wherein the reaction layer is provided with n reaction modules, and each reaction module is provided with a circulation passage, a reaction micro-cavity and a sample feeding opening by utilizing a multilayer soft photoetching technology. By adopting the chip, the circulation distance of the sample before being introduced into a small chamber is identical, the sample feeding quantity is identical, and an error caused by the non-uniformity of the reaction volume can be avoided. The experiment cost is reduced. The sample feeding speed is increased. Quantitative detection in real time can be realized. The chip is reasonable in design, simple in structure, simple to operate and low in production cost and can be applied in the real-time fluorescent quantitative nucleic acid amplification, digital nucleic acid amplification, single-cell separation of nucleic acid amplification, SNP detection, detection of single-base mutation, detection of copy number imbalance, research of single-cell gene expression profile, early detection of cancer marker, stem cell differentiation identification, cell separation identification and the like.

The invention relates to an experimental device and an experimental method for determining the soil respiration action in a drying process. The experimental device comprises a base plate, a transparent sealing cover, a silica gel drier, and a CO2 absorption bottle, and the transparent sealing cover is covered on the base plate; the center of the upper surface of the transparent sealing cover is provided with an air pumping / discharging port; the silica gel drier is suspended below the air pumping / discharging port; and the CO2 absorption bottle is arranged near the side of the base plate. The experimental method comprises the following steps of: placing experimental soil on the base plate, and filling a NaOH solution in the CO2 absorption bottle; covering the transparent sealing cover, and pumping by using a manual air pump; dripping a BaCl2 solution into the CO2 absorption bottle by using a pinhole injector; quickly taking the fixed absorption liquid and filtering, and dripping the unreacted NaOH solution by using HCL and taking phenolphthalein as an indicator; and calculating the amount of CO2 produced under the soil respiration action according to the amount of the reacted NaOH. The experimental method is easy and practicable, reduces equipment cost and is more suitable for small-scale test research of a laboratory.

The invention discloses a micro-fluidic chip, a preparation method thereof, a micro-fluidic device and a pathogenic bacteria detection method. The micro-fluidic chip comprises a chip layer, a channelstructure is arranged in the chip layer, and an array structure of which the surface is modified with an aptamer is arranged in the channel structure. The micro-fluidic device prepared by adopting themicro-fluidic chip can be used for rapid and high-sensitivity detection of pathogenic bacteria in food, and the detection effect is high in stability.

The invention provides a capillary micro-droplet metal ball detection method for a surface enhanced Raman spectrum. The method comprises the following steps: mixing noble metal nanometer sol and an organic solvent phase with the density greater than that of water, adding a to-be-detected object extracting liquid and performing severe oscillation; rapidly assembling the noble metal nanometer material on an oil-water interface to form micro-droplet metal balls with adjustable nanometer material gaps; absorbing the micro-droplet metal balls in the capillary by utilizing capillary action; puttingunder a Raman spectrometer and performing detection to acquire an SERS feature fingerprint signal of the to-be-detected object; correcting a Raman spectral signal of the to-be-detected object by taking feature peak of the organic solvent as internal standard. The method can be applied to single-phase or double-phase and single-component or multi-component detection of the water-soluble / oil-solubleto-be-detected object, the bottleneck of detection on the to-be-detected objects with different solubility in a complex sample is broken, an organic phase in an assembling system serves as internal standard and a square capillary are cleverly combined to realize quantitative detection, and the method is simple in manufacturing and convenient in operation.

The invention discloses a seeding quantity online measurement method and device of a precision planter. The method comprises the steps of: firstly, transmitting the movement information of kernels to capacitance signals by an upstream or a downstream capacitance sensor; secondly, carrying out frequency transmission on the capacitance signals, and carrying out mutual correlation treatment on the capacitance signals subjected to frequency transmission to obtain transition time; thirdly, obtaining movement average velocity according to the transition time, and the centre distance of the upstream capacitance sensor and the downstream capacitance sensor, and demarcating the capacitance signals subjected to frequency transmission to obtain the duty ratio of the kernels; and fourthly, obtaining instant mass flow according to the movement average velocity and the duty ratio of the kernels, and obtaining the seeding quantity of an unit area according to the instant mass flow, the moving velocity, the breadth and the number of lines of the planter. The invention realizes the online measurement of the seeding quantity of the precision planter.

The invention discloses a multiplex RT-PCR (reverse transcription-polymerase chain reaction) primer probe group for real-time fluorescent quantitative detection of four porcine epidemic diarrhea viruses. The multiplex RT-PCR primer probe group comprises an upstream primer, a downstream primer and a specific fluorescent probe of a porcine epidemic diarrhea virus M gene, an upstream primer, a downstream primer and a specific fluorescent probe of a swine transmissible gastroenteritis virus S gene; an upstream primer, a downstream primer and a specific fluorescent probe of a porcine rotavirus VP6 gene; and an upstream primer, a downstream primer and a specific fluorescent probe of a porcine D-type coronavirus M gene. The kit assembled on the basis of the primer probe group has the advantages of high sensitivity, high specificity, low pollution and real-time detection, and provides a reliable technology and product for early warning, early diagnosis and prevention and control monitoring of clinical diarrhea virus in a first-line pig farm.

The invention discloses a system for photoelectric detection of the amount of laid powder for rapid forming. The system comprises a powder flowing detection mechanism, a controller and a computer connected with the controller, wherein the powder flowing detection mechanism comprises a fixed support, an upper powder falling pipe, a lower powder falling pipe and a photoelectric sensor; the upper powder falling pipe, the lower powder falling pipe and the photoelectric sensor are fixedly connected to the fixed support, a light-pervious pipe is connected between the upper powder falling pipe and the lower powder falling pipe, the transmitting end and the receiving end of the photoelectric sensor are located at the two sides of the light-pervious pipe respectively, a timer is connected to the input end of the controller, and the signal output end of the photoelectric sensor is connected with the signal input end of the timer. The invention further discloses a method for photoelectric detection of the amount of laid powder for rapid forming. According to the system and method for photoelectric detection of the amount of laid powder for rapid forming, the method can be implemented conveniently, real-time quantitative detection of the amount of powder laid currently can be achieved, the amount of laid powder can be visual and adjustable, the powder, of the required amount, to be laid can be obtained conveniently, part forming precision and part forming quality can be improved, the practicability is high, and the system and method can be used and popularized conveniently.

The invention belongs to the technical field of detection, and specifically relates to an online detection device for abrasion loss of a radial sliding bearing based on pressure detection. The technical scheme is as follows: the online detection device comprises a stator, a rotor, a pressure sensor, a pressing block, a spring, a ball and a signal processor; the stator is coaxially fixedly connected with a sliding bearing block; the rotor is coaxially fixedly connected with a rotating shaft; the stator is provided with a radial through hole; the pressure sensor is fixedly arranged at an outer port of the radial through hole; the ball is arranged in the radial through hole and in contact with the rotor; the pressing block is arranged below the pressure sensor; the spring is arranged betweenthe pressing block and the ball; the pressure sensor is used for acquiring the change of the spring pressure, forming a monitoring signal and transmitting the monitoring signal to the signal processor; and the signal processor is used for analyzing the monitoring signal, converting the monitoring signal into a rotating shaft position variation, and outputting the rotating shaft position variationto a data acquisition device. The online detection device for the abrasion loss of the radial sliding bearing based on the pressure detection provided by the invention can realize the online detectionof the abrasion loss of the radial sliding bearing.

The invention relates to a kit for detecting presence of pathogen pseudomonas aeruginosa in patient samples such as clinical bacterial pneumonia, corneal ulcer, urinary tract infection, septicemia and the like and samples such as cosmetics, environmental monitoring objects and the like, in particular to a kit for early and quickly diagnosing pseudomonas aeruginosa infection by a real-time fluorescence quantitative polymerase chain reaction technique.

The invention discloses a loop-mediated isothermal amplification (LAMP) kit of vibrio cholerae and application of the loop-mediated isothermal amplification kit. The kit comprises LAMP primers, a 2*reaction buffering solution, Bst DNA (Deoxyribonucleic Acid) polymerase, a fluorescent visual detection reagent, ultrapure water and a vibrio cholera DNA template; the LAMP primers comprise outer primers F3 and B3 and inner primers FIP and BIP. Specificity detection and sensitivity detection prove that the LAMP detection kit can be used for monitoring reactions in real time and quantitatively detecting the vibrio cholerae; a detection result is rapidly and accurately obtained, and convenience is brought to simple, convenient and rapid detection of the vibrio cholerae.

The invention relates to a voltage fluctuation quantitative detection circuit / device. According to the voltage fluctuation quantitative detection circuit / device, a resistance bridge is adopted to be connected to the two ends of a to-be-detected object in parallel, in one bridge arm of the resistance bridge, an embedded capacitor is connected with a bridge arm resistor in parallel, and the resistance bridge and the embedded capacitor form a terminal voltage change rate detection circuit together; the output end of the terminal voltage change rate detection circuit is the output end of a terminal voltage change signal of the to-be-detected object, and a detected voltage change signal is output outward. The voltage fluctuation quantitative detection circuit / device is simple in circuit structure and scientific and reasonable in design. The rise and drop of the terminal voltage of the to-be-detected object can be detected, so that qualitative detection is achieved, and moreover, the continuous change of the voltage can be tracked and measured, so that the real-time quantitative detection of the terminal voltage of the to-be-detected object is detected.

The invention provides a 2-(10-buty1-2-methoxy-10H-phenothiazin-3-yl)-7-(diethylamino)chromenylium perchlorate derivative as well as a preparation method and application thereof. The 2-(10-buty1-2-methoxy-10H-phenothiazin-3-yl)-7-(diethylamino)chromenylium perchlorate derivative for real-time quantitative detection of HClO provided by the invention is simple and convenient to synthesize, cheap andeasily available in raw materials and good in water solubility, shows the advantages of high sensitivity, good selectivity and instantaneous response to HClO detection and can realize real-time quantitative detection of hypochlorous acid in a water phase system and living cells, and detection and quantification of HClO in the living cell and zebra fish in-vivo ferroptosis process are realized forthe first time, and the derivative has clinical application values.

The invention discloses a device for diagnosing diseases of a skeletal muscle system based on multi-physics field coupling and a method thereof, and belongs to the technical field of diagnosis of related diseases of the skeletal muscle system. The device is composed of a soft tissue mechanical modulus field and flow field measuring device, a soft tissue surface temperature measuring device, a man-machine interaction system and an examining table. According to the invention, soft tissues such as muscles, tendons, ligaments and blood vessels around the skeletal muscle system disease are used as test objects for the first time, and the lesion mechanism of the skeletal muscle system disease is explored; by means of the device, in-vivo, non-invasive, real-time and quantitative detection of multi-physics field parameters such as a mechanical modulus field, a flow field and a surface temperature field of soft tissues such as muscles, ligaments, tendons and blood vessel walls can be achieved, and through multi-physics field contrastive analysis and neural network deep learning, data support and theoretical basis are provided for quantitatively revealing a new lesion mechanism of the skeletal muscle system diseases, and meanwhile, an in-vivo noninvasive device and method are provided for early diagnosis of the clinical skeletal muscle system diseases.

The invention relates to a method for detecting alkaline phosphatase and cardiac troponin I in real time through in-situ fluorescence reaction initiated by copper ions and application. According to the method, dopamine and phenol (1, 3-naphthalene diphenol, 8-hydroxyjulolidine and 1, 5-naphthalene diphenol) are taken as raw materials, and a fluorescent compound (FCs) with high fluorescence intensity, good stability and adjustable wavelength is synthesized in a room-temperature water phase. Under the initiation of Cu <2+>, dopamine and different phenols can obtain the fluorescent substances with different emission wavelengths, and the affinity of pyrophosphate ions (PPi) to Cu <2+> is utilized to realize the fluorescence closing of a system. Under the catalytic hydrolysis of alkaline phosphatase (ALP), PPi can be quickly converted into phosphate ions, so that the fluorescence of the system is opened. A multi-channel immunofluorescence sensing platform is constructed by taking cardiac troponin I as a targeting antigen and taking alkaline phosphatase as a marker enzyme. In addition, a sensing system still shows rapid response and good recovery rate in human serum.

The invention relates to a real-time fluorescent PCR detection kit, in particular to a kit for diagnosing human enteric virus infection early and quickly by real-time fluorescent quantitative polymerase chain reaction technique. The kit has quite high sensitivity and specificity, and can realize quick and early diagnosis and quantitative analysis of human enteric viruses in samples of feces, serum, throat swab, cerebrospinal fluid, herpes fluid and the like.

The invention relates to a radial sliding bearing wear on-line measuring device based on inductance measurement. The technical scheme is characterized in that the device comprises a stator, a rotor, acoil, an iron core, a spring, a roll ball and a signal processor. The stator is coaxially and fixedly connected with a sliding bearing pedestal; the rotor is coaxially and fixedly connected with a rotating shaft; the stator is provided with a radial blind hole; the coil is coaxially fixed outside of the radial blind hole; the spring is arranged in the radial blind hole; the iron core is arrangedin the radial blind hole and is tightly attached to the spring and coaxial with the coil; and the roll ball is arranged in the radial blind hole and between the iron core and the rotor. The coil is used for collecting position change of the iron core, forming a monitoring signal and sending the monitoring signal to the signal processor; and the signal processor is used for analyzing the monitoringsignal and converting the signal into position change amount of the rotating shaft and outputting the position change amount to a data acquisition device. The device can realize on-line measurement of wear of a radial sliding bearing.

The present invention relates to a fluorescence detection device based on a micro-fluidic chip and a micro fluidic chip detection system, the fluorescence detection device comprises: a base; at leasttwo light path assemblies fixedly arranged on the base, and each of the at least two light path assemblies comprising an excitation light path module and a collection light path module; a first bracket movably arranged on the base; a first optical fiber whose first end penetrates through and is fixed on the first bracket and second end is used for being close to the chip and transmitting light tothe chip; and a second optical fiber whose first end of the penetrates through and is fixed to the first support and second end is used for being close to the chip and recycling light in the chip. Thefirst end of the first optical fiber and the first end of the second optical fiber are configured to move along with the first bracket and are selectively and correspondingly connected with an excitation light path module and a collection light path module in one of the at least two light path assemblies respectively. According to the invention, switching of a plurality of required light can be autonomously controlled in the biochemical reaction process.

The invention provides a fluorescent probe based on a chalcone structure, a preparation method and its application in detecting hydrazine. The probe specifically involved is 3-(3'-(2'-chloro-7'-(diethylamino)quinolyl)allyl)-7-diethylaminocoumarin (QCT-Cl). The probe QCT‑Cl is prepared from 2‑chloro‑7‑diethylaminoquinoline formaldehyde and 7‑diethylaminocoumarin‑3‑ethanone through condensation reaction, with mild synthesis conditions and simple purification . In the present invention, QCT-Cl is a fluorescence-enhanced fluorescent probe. After hydrazine interacts with it, its solution can be seen by the naked eye under an ultraviolet lamp from weak red to bright blue. It has high selectivity, strong anti-interference, and sensitivity. High stability, the lowest detection limit of hydrazine is 9.46×10 ‑8 mol / L, it can be used as a fluorescent probe for the qualitative and quantitative detection of hydrazine, and is widely used in the detection of hydrazine in the environmental and biological fields.

The invention provides a reactive fluorescent probe, its preparation method and its application in detecting hydrazine. The probe specifically involved is 2-(2-chloro-7-diethylaminoquinolin-3-yl)methylenemalononitrile (QAM). It is prepared by condensation reaction using 2-chloro-7-diethylaminoquinoline-3-formaldehyde and malononitrile as raw materials. After different concentrations of hydrazine interacted with QAM, under 365nm ultraviolet light, the solution containing QAM could be seen with the naked eye, and the filter paper sheet and thin-layer silica gel plate changed from orange-yellow fluorescence to blue-green fluorescence. The minimum detection limit of hydrazine by fluorescence spectrophotometry is 3.9849×10 ‑9 mol / L. The invention can qualitatively and quantitatively detect hydrazine in samples from different sources by fluorescence spectrophotometry, does not need complicated devices and professional technicians during the detection, is convenient to operate, low in cost, fast in response speed and high in sensitivity.

The invention provides a fluorescent probe based on a chalcone structure, a preparation method and application of the fluorescent probe in hydrazine detection. In particular, the related probe is 3-(3 '-(2'-chloro-7 '-(diethylamino) quinolyl) allyl acetone)-7-diethylamino coumarin (QCT-Cl). The probe QCT-Cl is prepared by taking 2-chloro-7-diethylaminoquinoline formaldehyde and 7-diethylaminocoumarin-3-ethanone as raw materials through condensation reaction, the synthesis condition is mild, and the purification is simple. The QCT-Cl is a fluorescence enhanced fluorescent probe, after hydrazine acts on the QCT-Cl, it can be seen that a solution of the QCT-Cl changes into brilliant blue from weak red under an ultraviolet lamp through naked eyes, the QCT-Cl has high selectivity, high anti-interference performance and high sensitivity, the lowest detection limit of hydrazine detection is 9.46 * 10 <-8 > mol / L, and the QCT-Cl can be used as a fluorescent probe for qualitative and quantitative hydrazine detection. The probe is widely applied to hydrazine detection in environmental and biological fields.

The invention provides a capillary micro-droplet metal ball detection method for a surface enhanced Raman spectrum. The method comprises the following steps: mixing noble metal nanometer sol and an organic solvent phase with the density greater than that of water, adding a to-be-detected object extracting liquid and performing severe oscillation; rapidly assembling the noble metal nanometer material on an oil-water interface to form micro-droplet metal balls with adjustable nanometer material gaps; absorbing the micro-droplet metal balls in the capillary by utilizing capillary action; puttingunder a Raman spectrometer and performing detection to acquire an SERS feature fingerprint signal of the to-be-detected object; correcting a Raman spectral signal of the to-be-detected object by taking feature peak of the organic solvent as internal standard. The method can be applied to single-phase or double-phase and single-component or multi-component detection of the water-soluble / oil-solubleto-be-detected object, the bottleneck of detection on the to-be-detected objects with different solubility in a complex sample is broken, an organic phase in an assembling system serves as internal standard and a square capillary are cleverly combined to realize quantitative detection, and the method is simple in manufacturing and convenient in operation.

The invention belongs to the field of animal immune detection, and in particular relates to a test paper card for quantitative detection of swine fever virus antibody with double antigen sandwiches and double detection lines. The test card includes sample pad, classical swine fever virus E2 protein gold standard pad, rabbit IgG gold standard pad, absorbent pad, double detection line coated with classical swine fever virus E2a protein, and nitrocellulose membrane of goat anti-rabbit IgG quality control line and PVC rubber sheet; colloidal gold adopts double-antigen sandwich and double detection line; the classical swine fever virus gold standard pad is coated with the protein antigen secreted by Escherichia coli BL21 / pE2 with the preservation number CCTCC NO: M2018060. E2 antigen and gold-labeled rabbit IgG; the nitrocellulose membrane is coated with the CSFV E2a antigen constructed from the protein antigen secreted by Escherichia coli BL21 / pE2a with the preservation number CCTCC NO: M2018059, constituting a double detection line area and The quality control line region is composed of goat anti-rabbit IgG.

The invention belongs to the technical field of bioanalysis and particularly relates to a kit for detecting polynucleotide kinase (PNK) and a detection method for the kit. The kit disclosed by the invention comprises a probe A, a probe S, a mixed solution A, a template DNA (Deoxyribonucleic Acid), lambda exonucleautomotive service engineers, a polynucleotide kinase standard substance and 1xNEBuffer and 10mM triphosadenine. The detection method for the kit disclosed by the invention has the advantages of simple operation, reduction of detection cost, high sensitivity and high specificity, and can be applied to detection of complex samples. In addition, the kit disclosed by the invention is also applied to PNK activity inhibitory detection.

The invention discloses a photoelectric detection method for rapid prototyping powder spreading. The system adopted includes a powder flow detection mechanism, a controller and a computer. A sensor, a light-transmitting tube and a timer; the method includes the steps of: 1. opening a tube hole on the powder spreading platform, and fixing the upper and lower powder tubes at the tube hole; The signal is detected, and the timer transmits the timing of the powder flowing through the light-transmitting tube to the controller; 3. The controller substitutes the time of powder flowing through the light-transmitting tube into the amount of powder spread and the powder flowing through the light-transmitting tube. In the relational model of the tube time, the amount of powder spread is calculated and transmitted to the computer for display. The present invention can realize the real-time quantitative detection of the current powder coating amount, can make the powder coating amount visualized and correctable, facilitates the required powder coating amount, and can improve the forming accuracy and quality of parts.

The invention discloses a microfluidic chip, a preparation method thereof, a microfluidic device and a detection method for pathogenic bacteria. The microfluidic chip includes a chip layer, a channel structure inside the chip layer, and an array structure with aptamers modified on the surface inside the channel structure. The microfluidic device further prepared by using the microfluidic chip can be used for rapid and highly sensitive detection of pathogenic bacteria in food, and the detection effect is highly stable.

The invention provides a method for detecting exocysts in a liquid sample to be tested, the liquid sample to be tested contains exocysts; the method comprises: passing the liquid sample to be tested on the surface of a microarray chip for contacting, the microarray chip The surface of the array chip is loaded with one or more specific antibodies to exocyst surface membrane proteins, and the surface plasmon resonance signal change value of the microarray chip before and after contacting the liquid sample to be tested is obtained; the surface of the microarray chip The change value of the plasmon resonance signal indicates the amount of exocysts in the liquid sample to be tested. The method provided by the present invention is based on the surface plasmon resonance imaging technology, does not need a complicated purification process for the liquid sample to be tested, and can accurately and reliably detect the internal and external cysts in the liquid sample quantitatively in real time.

The invention provides a method for detecting external capsule bodies in a liquid sample. The liquid sample to be detected contains the external capsule bodies. The method comprises the steps of feeding the liquid sample to be detected into the surface of a microarray chip for being in contact with each other, wherein the surface of the microaray chip is loaded with one or more kinds of specific antibodies with external capsule body surface membrane proteins so as to obtain surface plasma resonance signal change values before and after the microarray chip is in the contact with the liquid sample to be detected; and indicating the quantity of the external capsule bodies in the liquid sample to be detected according to the surface plasma resonance signal change values of the microarray chip. The method provided by the invention is based on a surface plasma resonance imaging technology, so that the liquid sample to be detected does not need to be subjected to complicated purification, and the external capsule bodies in the liquid sample to be detected can be accurately, reliably and quantitatively detected in real time.