31results about How to "Real-time quantitative detection" patented technology

The invention provides a high-integration equidistance equipartition nucleic acid amplification micro-fluidic chip. The high-integration equidistance equipartition nucleic acid amplification micro-fluidic chip sequentially consists of a cover glass layer, a reaction layer, a decorative layer and a sealing layer from bottom to top in a thermal bonding manner, wherein the reaction layer is provided with n reaction modules, and each reaction module is provided with a circulation passage, a reaction micro-cavity and a sample feeding opening by utilizing a multilayer soft photoetching technology. By adopting the chip, the circulation distance of the sample before being introduced into a small chamber is identical, the sample feeding quantity is identical, and an error caused by the non-uniformity of the reaction volume can be avoided. The experiment cost is reduced. The sample feeding speed is increased. Quantitative detection in real time can be realized. The chip is reasonable in design, simple in structure, simple to operate and low in production cost and can be applied in the real-time fluorescent quantitative nucleic acid amplification, digital nucleic acid amplification, single-cell separation of nucleic acid amplification, SNP detection, detection of single-base mutation, detection of copy number imbalance, research of single-cell gene expression profile, early detection of cancer marker, stem cell differentiation identification, cell separation identification and the like.

The invention relates to an experimental device and an experimental method for determining the soil respiration action in a drying process. The experimental device comprises a base plate, a transparent sealing cover, a silica gel drier, and a CO2 absorption bottle, and the transparent sealing cover is covered on the base plate; the center of the upper surface of the transparent sealing cover is provided with an air pumping/discharging port; the silica gel drier is suspended below the air pumping/discharging port; and the CO2 absorption bottle is arranged near the side of the base plate. The experimental method comprises the following steps of: placing experimental soil on the base plate, and filling a NaOH solution in the CO2 absorption bottle; covering the transparent sealing cover, and pumping by using a manual air pump; dripping a BaCl2 solution into the CO2 absorption bottle by using a pinhole injector; quickly taking the fixed absorption liquid and filtering, and dripping the unreacted NaOH solution by using HCL and taking phenolphthalein as an indicator; and calculating the amount of CO2 produced under the soil respiration action according to the amount of the reacted NaOH. The experimental method is easy and practicable, reduces equipment cost and is more suitable for small-scale test research of a laboratory.

The invention discloses a multiplex RT-PCR (reverse transcription-polymerase chain reaction) primer probe group for real-time fluorescent quantitative detection of four porcine epidemic diarrhea viruses. The multiplex RT-PCR primer probe group comprises an upstream primer, a downstream primer and a specific fluorescent probe of a porcine epidemic diarrhea virus M gene, an upstream primer, a downstream primer and a specific fluorescent probe of a swine transmissible gastroenteritis virus S gene; an upstream primer, a downstream primer and a specific fluorescent probe of a porcine rotavirus VP6 gene; and an upstream primer, a downstream primer and a specific fluorescent probe of a porcine D-type coronavirus M gene. The kit assembled on the basis of the primer probe group has the advantages of high sensitivity, high specificity, low pollution and real-time detection, and provides a reliable technology and product for early warning, early diagnosis and prevention and control monitoring of clinical diarrhea virus in a first-line pig farm.

The invention discloses a system for photoelectric detection of the amount of laid powder for rapid forming. The system comprises a powder flowing detection mechanism, a controller and a computer connected with the controller, wherein the powder flowing detection mechanism comprises a fixed support, an upper powder falling pipe, a lower powder falling pipe and a photoelectric sensor; the upper powder falling pipe, the lower powder falling pipe and the photoelectric sensor are fixedly connected to the fixed support, a light-pervious pipe is connected between the upper powder falling pipe and the lower powder falling pipe, the transmitting end and the receiving end of the photoelectric sensor are located at the two sides of the light-pervious pipe respectively, a timer is connected to the input end of the controller, and the signal output end of the photoelectric sensor is connected with the signal input end of the timer. The invention further discloses a method for photoelectric detection of the amount of laid powder for rapid forming. According to the system and method for photoelectric detection of the amount of laid powder for rapid forming, the method can be implemented conveniently, real-time quantitative detection of the amount of powder laid currently can be achieved, the amount of laid powder can be visual and adjustable, the powder, of the required amount, to be laid can be obtained conveniently, part forming precision and part forming quality can be improved, the practicability is high, and the system and method can be used and popularized conveniently.

The invention provides a 2-(10-buty1-2-methoxy-10H-phenothiazin-3-yl)-7-(diethylamino)chromenylium perchlorate derivative as well as a preparation method and application thereof. The 2-(10-buty1-2-methoxy-10H-phenothiazin-3-yl)-7-(diethylamino)chromenylium perchlorate derivative for real-time quantitative detection of HClO provided by the invention is simple and convenient to synthesize, cheap andeasily available in raw materials and good in water solubility, shows the advantages of high sensitivity, good selectivity and instantaneous response to HClO detection and can realize real-time quantitative detection of hypochlorous acid in a water phase system and living cells, and detection and quantification of HClO in the living cell and zebra fish in-vivo ferroptosis process are realized forthe first time, and the derivative has clinical application values.

The invention relates to a radial sliding bearing wear on-line measuring device based on inductance measurement. The technical scheme is characterized in that the device comprises a stator, a rotor, acoil, an iron core, a spring, a roll ball and a signal processor. The stator is coaxially and fixedly connected with a sliding bearing pedestal; the rotor is coaxially and fixedly connected with a rotating shaft; the stator is provided with a radial blind hole; the coil is coaxially fixed outside of the radial blind hole; the spring is arranged in the radial blind hole; the iron core is arrangedin the radial blind hole and is tightly attached to the spring and coaxial with the coil; and the roll ball is arranged in the radial blind hole and between the iron core and the rotor. The coil is used for collecting position change of the iron core, forming a monitoring signal and sending the monitoring signal to the signal processor; and the signal processor is used for analyzing the monitoringsignal and converting the signal into position change amount of the rotating shaft and outputting the position change amount to a data acquisition device. The device can realize on-line measurement of wear of a radial sliding bearing.

The present invention relates to a fluorescence detection device based on a micro-fluidic chip and a micro fluidic chip detection system, the fluorescence detection device comprises: a base; at leasttwo light path assemblies fixedly arranged on the base, and each of the at least two light path assemblies comprising an excitation light path module and a collection light path module; a first bracket movably arranged on the base; a first optical fiber whose first end penetrates through and is fixed on the first bracket and second end is used for being close to the chip and transmitting light tothe chip; and a second optical fiber whose first end of the penetrates through and is fixed to the first support and second end is used for being close to the chip and recycling light in the chip. Thefirst end of the first optical fiber and the first end of the second optical fiber are configured to move along with the first bracket and are selectively and correspondingly connected with an excitation light path module and a collection light path module in one of the at least two light path assemblies respectively. According to the invention, switching of a plurality of required light can be autonomously controlled in the biochemical reaction process.

The invention provides a fluorescent probe based on a chalcone structure, a preparation method and application of the fluorescent probe in hydrazine detection. In particular, the related probe is 3-(3 '-(2'-chloro-7 '-(diethylamino) quinolyl) allyl acetone)-7-diethylamino coumarin (QCT-Cl). The probe QCT-Cl is prepared by taking 2-chloro-7-diethylaminoquinoline formaldehyde and 7-diethylaminocoumarin-3-ethanone as raw materials through condensation reaction, the synthesis condition is mild, and the purification is simple. The QCT-Cl is a fluorescence enhanced fluorescent probe, after hydrazine acts on the QCT-Cl, it can be seen that a solution of the QCT-Cl changes into brilliant blue from weak red under an ultraviolet lamp through naked eyes, the QCT-Cl has high selectivity, high anti-interference performance and high sensitivity, the lowest detection limit of hydrazine detection is 9.46 * 10 <-8 > mol/L, and the QCT-Cl can be used as a fluorescent probe for qualitative and quantitative hydrazine detection. The probe is widely applied to hydrazine detection in environmental and biological fields.