Microfluidic chip and its preparation method, microfluidic device and detection method of pathogenic bacteria

Abstract

The invention discloses a microfluidic chip, a preparation method thereof, a microfluidic device and a detection method for pathogenic bacteria. The microfluidic chip includes a chip layer, a channel structure inside the chip layer, and an array structure with aptamers modified on the surface inside the channel structure. The microfluidic device further prepared by using the microfluidic chip can be used for rapid and highly sensitive detection of pathogenic bacteria in food, and the detection effect is highly stable.

Description

technical field [0001] The invention relates to a microfluidic chip and a preparation method thereof, a microfluidic device, and a detection method of pathogenic bacteria. Background technique [0002] Food poisoning caused by foodborne pathogenic microorganisms is a food safety issue of global concern. According to the estimates of the World Health Organization, more than 70% of the annual food-borne diseases in the world are caused by pathogenic microorganisms. In recent years, according to statistics from the National Health and Family Planning Office and the National Center for Disease Control and Prevention, the number of food poisoning caused by pathogenic microorganisms has always ranked first in food poisoning incidents. China is the largest food consumer, producer and importer. It is an urgent task for us to improve the detection level of food-borne pathogenic microorganisms and the ability to respond to food safety emergencies. Fast-reading, accurate and sensitiv...

AI Technical Summary

Problems solved by technology

Existing detection technologies (microbial testing, immunological testing, and molecular biology techniques, etc.) have their own shortcomings and face many problems: complex operation process, long detection time, low detection target coverage, high false positives, suitable for on-site There are fewer instruments and reagents for detection, etc.

[0004] Existing devices or methods for detecting foodborne pathogenic bacteria using microfluidics have problems such as low species specificity and slow detection time. For example, CN109813695A discloses a microfluidic chip-based microbial detection system and Its detection method, which uses sound waves to separate and enrich bacteria of different diameters, has the problem of poor specificity

[0005] CN107904161A discloses a microfluidic chip for visual real-time detection of pathogen nucleic acid and its preparation method and detection method. It uses immune magnetic beads to capture pathogenic bacteria. Since the immune magnetic beads can only be fixed on the bottom of the chip channel, for The ability to capture pathogenic bacteria in the upper solution is limited, and the capture efficiency of the microfluidic chip of the immunomagnetic beads does not exceed 50%

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