Method for detecting alkaline phosphatase and cardiac troponin I in real time through in-situ fluorescence reaction initiated by copper ions and application

A cardiac troponin and fluorescent reaction technology, applied in the field of nano-bio-sensing, can solve the problems of long response time, low sensitivity, complicated steps, etc., and achieve the effects of fast response, good stability and good applicability
CN113702630AActive Publication Date: 2021-11-26NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2021-11-26

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Abstract

The invention relates to a method for detecting alkaline phosphatase and cardiac troponin I in real time through in-situ fluorescence reaction initiated by copper ions and application. According to the method, dopamine and phenol (1, 3-naphthalene diphenol, 8-hydroxyjulolidine and 1, 5-naphthalene diphenol) are taken as raw materials, and a fluorescent compound (FCs) with high fluorescence intensity, good stability and adjustable wavelength is synthesized in a room-temperature water phase. Under the initiation of Cu <2+>, dopamine and different phenols can obtain the fluorescent substances with different emission wavelengths, and the affinity of pyrophosphate ions (PPi) to Cu <2+> is utilized to realize the fluorescence closing of a system. Under the catalytic hydrolysis of alkaline phosphatase (ALP), PPi can be quickly converted into phosphate ions, so that the fluorescence of the system is opened. A multi-channel immunofluorescence sensing platform is constructed by taking cardiac troponin I as a targeting antigen and taking alkaline phosphatase as a marker enzyme. In addition, a sensing system still shows rapid response and good recovery rate in human serum.
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Description

Technical field

[0001] The present invention relates to the construction of the in-situ fluorescent immunoassay sensing platform based on the wavelength of copper ion triggering and its analysis, for real-time sensitive detection CTNI, belonging to the field of nanohosphere sensing. Background technique

[0002] Alkaline phosphatase (ALP) is a membrane binding enzyme, which is widely expressed in liver, bone, intestinal, placenta, kidney. It is mainly responsible for hydrolyzing and transforming monophosphate from various proteins and non-protein substrates. As a recognized biomarker, alkaline phosphatase plays an important role in many important physiological and pathological processes such as cell cycle growth, apoptosis and signal transduction pathways. The normal value of adult serum ALP is generally in 46 ~ 190u / L, children and pregnant women ALP normal value above 500u / l, but serum ALP abnormalities is close to bone disease, liver function disorders, breast cancer, pros...

Claims

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