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Method for detecting l,5-anhydroglucitol based on persimmon tannin composite nano material

A composite nanomaterial, 5-AG technology, applied in the analysis of materials, material electrochemical variables, material analysis by electromagnetic means, etc., can solve the problems of expensive reagents, cumbersome and complicated operations, and achieve simple operation and specific surface area. Large, easily miniaturized effects

Active Publication Date: 2019-08-20
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

The determination of 1,5-AG by whole enzyme method and liquid chromatography-mass spectrometry has the characteristics of sensitivity, accuracy, and high specificity, but these methods are cumbersome and complicated to operate, and the cost of reagents is expensive, so special instruments are required for detection

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  • Method for detecting l,5-anhydroglucitol based on persimmon tannin composite nano material
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  • Method for detecting l,5-anhydroglucitol based on persimmon tannin composite nano material

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Embodiment Construction

[0041] The present invention will be described in detail below in combination with specific embodiments. This embodiment is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any form. Any simple modifications made to the above embodiments according to the technical essence of the present invention, equivalent changes and modifications, all belong to this invention. within the scope of the technical solution of the invention.

[0042] figure 1 It is a schematic diagram of detecting 1,5-AG based on persimmon tannin composite nanomaterials. First, graphene oxide was reduced to reduced graphene oxide (RGO) by ascorbic acid, and then RGO / PT / Pt-Pd NPs were directly prepared by a one-step reduction method using persimmon tannin as raw material. Secondly, gold nanoparticles (Au NPs / SPE) were deposited on the surface of the screen-printed electrode by electrodeposition technology, and the RGO / PT / Pt-Pd NPs dropped on the electr...

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Abstract

The invention relates to a method for detecting l,5-AG based on a persimmon tannin composite nano material. The method comprises the steps of the preparation of a composite nano material, the activation and modification of a silk-screen printing electrode, and the construction of a biological sensing interface. According to the method, H2O2 is generated by means of the signal amplification and excellent electron transfer effect of RGO / PT / Pt-Pd NPs, and the specific catalysis effect of PROD on the l,5-AG; the H2O2 is subjected to the catalytic decomposition of the RGO / PT / Pt-Pd NPs; generated electrons are transferred onto the surface of the electrode through the RGO / PT / Pt-Pd NP composite nano film; current response signals are measured through DPV; a working curve is drawn according to a relation between the concentration of the l,5-AG and the response current of a sensor; and the detection of the l,5-AG is realized.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a method for detecting 1,5-anhydroglucitol by modifying a screen printing electrode with a composite nanometer material. Background technique [0002] l,5-Anhydroglucitol (1,5-Anhydroglucitol, 1,5-AG) is a polyol formed by the deoxygenation of the first carbon in the pyranose ring structure. 1,5-AG can be used as an indicator for blood sugar control monitoring in the recent 3 to 7 days. In the early days, 1,5-AG was mainly gas chromatography and liquid chromatography. The special instruments for this method were expensive, and the operation was very cumbersome and time-consuming, so it was not suitable for routine clinical testing. At present, the detection methods of serum 1,5-AG mainly include whole-enzyme method and liquid chromatography-mass spectrometry (LC / MS) method, etc. The whole enzyme method realizes the detection of 1,5-AG through enzymatic reaction, ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/3272G01N27/3277G01N27/3278
Inventor 李桂银赵乐周治德王仲民梁晋涛
Owner GUILIN UNIV OF ELECTRONIC TECH
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