Heating cycle controlled polymerase chain reaction biological detection system

A biological detection system and chain reaction technology, which is applied in the field of thermal cycle control polymerase chain reaction biological detection system, can solve the problems of difficulty in consistent control of amplification conditions, difficulty in obtaining reliable results, easy access to flat slope areas, etc. , to achieve the effect of large dynamic range, low power consumption and convenient use
CN1800411AInactive Publication Date: 2006-07-12INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
Publication Date
2006-07-12
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention relates to a heat cycle control polyase chain reaction biological detection system which is formed by a heat cycle heating freezing component (1), an optical system (2), an electron-multiplier phototube (3), a moving table (4), a step motor (5), a heat cycle temperature controller (6), a fluorescence detecting controller (7), a step motor driver (8) and a PC machine (9). It adopts fluorescence real-time detecting mode to analyze the augmentation of PCR form; it adopts thermoelectric semiconductor cryogenic technique to achieve PCR course and uses high-fidelity photoelectric system to detect the fluorescence signal; it adopts micro machine as upper machine to do heat cycle craft controlling and adopts light intensity to real-time detect the PCR biology detecting system.
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Description

Technical field

[0001] The invention relates to a biological detection system with thermal cycle process control and light intensity real-time detection, in particular to a thermal cycle control polymerase chain reaction (PCR) biological detection system. technical background

[0002] PCR is the English abbreviation of "polymerase chain reaction", which is a new technique of gene detection with a wide range of uses. It can sensitively detect the presence of a specific gene and its content. PCR has broad application prospects in various fields such as medicine, industry and agriculture, archaeology, public security, and military affairs. PCR reaction is an in vitro rapid amplification technology that simulates natural DNA replication. Through test tube reaction, a specific gene fragment in a very small amount of genomic DNA sample can obtain millions of copies of a specific DNA sequence in a short period of time. PCR is repeated Thermal denaturation-annealing-primer extensi...

Claims

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