Heating cycle controlled polymerase chain reaction biological detection system

Abstract

The invention relates to a heat cycle control polyase chain reaction biological detection system which is formed by a heat cycle heating freezing component (1), an optical system (2), an electron-multiplier phototube (3), a moving table (4), a step motor (5), a heat cycle temperature controller (6), a fluorescence detecting controller (7), a step motor driver (8) and a PC machine (9). It adopts fluorescence real-time detecting mode to analyze the augmentation of PCR form; it adopts thermoelectric semiconductor cryogenic technique to achieve PCR course and uses high-fidelity photoelectric system to detect the fluorescence signal; it adopts micro machine as upper machine to do heat cycle craft controlling and adopts light intensity to real-time detect the PCR biology detecting system.

Description

Technical field [0001] The invention relates to a biological detection system with thermal cycle process control and light intensity real-time detection, in particular to a thermal cycle control polymerase chain reaction (PCR) biological detection system. technical background [0002] PCR is the English abbreviation of "polymerase chain reaction", which is a new technique of gene detection with a wide range of uses. It can sensitively detect the presence of a specific gene and its content. PCR has broad application prospects in various fields such as medicine, industry and agriculture, archaeology, public security, and military affairs. PCR reaction is an in vitro rapid amplification technology that simulates natural DNA replication. Through test tube reaction, a specific gene fragment in a very small amount of genomic DNA sample can obtain millions of copies of a specific DNA sequence in a short period of time. PCR is repeated Thermal denaturation-annealing-primer extensi...

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Problems solved by technology

[0003] (1) Subtle conditional fluctuations will affect the amount of amplification, and it is easy to enter the flat slope area when the reaction reaches a certain level;

[0004] (2) It is difficult to obtain reliable results by using the endpoint method to calculate the amount of template in the starting sample from the amount of DNA generated by amplification;

[0005] (3) Since it is not known whether it is in the flat slope area when the set number of cycles is reached, it is difficult to control the amplification conditions in each tube consistently

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